Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken
Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-ol...
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| Format: | Article |
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Sociedade Brasileira de Microbiologia
2017
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| Online Access: | https://eprints.nottingham.ac.uk/45100/ |
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| author | Barbosa, Fernanda de Oliveira Freitas Neto, Oliveiro Caetano de Batista, Diego Felipe Alves Almeida, Adriana Maria de Rubio, Marcela da Silva Alves, Lucas Bocchini Rodrigues Vasconcelos, Rosemeire de Oliveira Barrow, Paul Andrew Berchieri Junior, Angelo |
| author_facet | Barbosa, Fernanda de Oliveira Freitas Neto, Oliveiro Caetano de Batista, Diego Felipe Alves Almeida, Adriana Maria de Rubio, Marcela da Silva Alves, Lucas Bocchini Rodrigues Vasconcelos, Rosemeire de Oliveira Barrow, Paul Andrew Berchieri Junior, Angelo |
| author_sort | Barbosa, Fernanda de Oliveira |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE ΔmotB) or non-flagellated (SE ΔfliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes. SE ΔmotB and SE ΔfliC were recovered in significantly lower numbers from caecal contents in comparison with Salmonella Enteritidis at early stages of infection (3 and 5 dpi). The SE ΔmotB strain, which synthesises paralysed flagella, showed poorer intestinal colonisation ability than the non-flagellated SE ΔfliC. Histopathological analyses demonstrated that the flagellated strains induced more intense lymphoid reactivity in liver, ileum and caeca. Thus, in the present study the flagellar structure and motility seemed to play a role in the early stages of the intestinal colonisation by Salmonella Enteritidis in the chicken. |
| first_indexed | 2025-11-14T19:58:00Z |
| format | Article |
| id | nottingham-45100 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:58:00Z |
| publishDate | 2017 |
| publisher | Sociedade Brasileira de Microbiologia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-451002020-05-04T18:48:59Z https://eprints.nottingham.ac.uk/45100/ Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken Barbosa, Fernanda de Oliveira Freitas Neto, Oliveiro Caetano de Batista, Diego Felipe Alves Almeida, Adriana Maria de Rubio, Marcela da Silva Alves, Lucas Bocchini Rodrigues Vasconcelos, Rosemeire de Oliveira Barrow, Paul Andrew Berchieri Junior, Angelo Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE ΔmotB) or non-flagellated (SE ΔfliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes. SE ΔmotB and SE ΔfliC were recovered in significantly lower numbers from caecal contents in comparison with Salmonella Enteritidis at early stages of infection (3 and 5 dpi). The SE ΔmotB strain, which synthesises paralysed flagella, showed poorer intestinal colonisation ability than the non-flagellated SE ΔfliC. Histopathological analyses demonstrated that the flagellated strains induced more intense lymphoid reactivity in liver, ileum and caeca. Thus, in the present study the flagellar structure and motility seemed to play a role in the early stages of the intestinal colonisation by Salmonella Enteritidis in the chicken. Sociedade Brasileira de Microbiologia 2017-06-07 Article PeerReviewed Barbosa, Fernanda de Oliveira, Freitas Neto, Oliveiro Caetano de, Batista, Diego Felipe Alves, Almeida, Adriana Maria de, Rubio, Marcela da Silva, Alves, Lucas Bocchini Rodrigues, Vasconcelos, Rosemeire de Oliveira, Barrow, Paul Andrew and Berchieri Junior, Angelo (2017) Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken. Brazilian Journal of Microbiology . ISSN 1517-8382 Mutation ; Flagellum ; Virulence factors ; Poultry ; Gut colonisation http://www.sciencedirect.com/science/article/pii/S1517838216307080?via%3Dihub doi:10.1016/j.bjm.2017.01.012 doi:10.1016/j.bjm.2017.01.012 |
| spellingShingle | Mutation ; Flagellum ; Virulence factors ; Poultry ; Gut colonisation Barbosa, Fernanda de Oliveira Freitas Neto, Oliveiro Caetano de Batista, Diego Felipe Alves Almeida, Adriana Maria de Rubio, Marcela da Silva Alves, Lucas Bocchini Rodrigues Vasconcelos, Rosemeire de Oliveira Barrow, Paul Andrew Berchieri Junior, Angelo Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
| title | Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
| title_full | Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
| title_fullStr | Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
| title_full_unstemmed | Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
| title_short | Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
| title_sort | contribution of flagella and motility to gut colonisation and pathogenicity of salmonella enteritidis in the chicken |
| topic | Mutation ; Flagellum ; Virulence factors ; Poultry ; Gut colonisation |
| url | https://eprints.nottingham.ac.uk/45100/ https://eprints.nottingham.ac.uk/45100/ https://eprints.nottingham.ac.uk/45100/ |