Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model
A key feature of the fowl-specific pathogen Salmonella Pullorum is its vertical transmission to progeny via the egg. In this study, PCR signature-tagged mutagenesis identified nine genes of a strain of S. Pullorum that contributed to survival in the chicken embryo during incubation. The genes were i...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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Elsevier
2017
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| Online Access: | https://eprints.nottingham.ac.uk/45099/ |
| _version_ | 1848797068747866112 |
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| author | Geng, Shizhong Tian, Qin Guo, Rongxian Jiao, Yang Barrow, Paul Yin, Chao Wang, Yaonan Geng, Haopeng Pan, Zhiming Jiao, Xinan |
| author_facet | Geng, Shizhong Tian, Qin Guo, Rongxian Jiao, Yang Barrow, Paul Yin, Chao Wang, Yaonan Geng, Haopeng Pan, Zhiming Jiao, Xinan |
| author_sort | Geng, Shizhong |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | A key feature of the fowl-specific pathogen Salmonella Pullorum is its vertical transmission to progeny via the egg. In this study, PCR signature-tagged mutagenesis identified nine genes of a strain of S. Pullorum that contributed to survival in the chicken embryo during incubation. The genes were involved in invasion, cell division, metabolism and bacterial defence. The competition index in vivo and in vitro together with a virulence evaluation for chicken embryos of all nine mutant strains confirmed their attenuation. |
| first_indexed | 2025-11-14T19:58:00Z |
| format | Article |
| id | nottingham-45099 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:58:00Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-450992020-05-04T18:32:25Z https://eprints.nottingham.ac.uk/45099/ Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model Geng, Shizhong Tian, Qin Guo, Rongxian Jiao, Yang Barrow, Paul Yin, Chao Wang, Yaonan Geng, Haopeng Pan, Zhiming Jiao, Xinan A key feature of the fowl-specific pathogen Salmonella Pullorum is its vertical transmission to progeny via the egg. In this study, PCR signature-tagged mutagenesis identified nine genes of a strain of S. Pullorum that contributed to survival in the chicken embryo during incubation. The genes were involved in invasion, cell division, metabolism and bacterial defence. The competition index in vivo and in vitro together with a virulence evaluation for chicken embryos of all nine mutant strains confirmed their attenuation. Elsevier 2017-03-01 Article PeerReviewed Geng, Shizhong, Tian, Qin, Guo, Rongxian, Jiao, Yang, Barrow, Paul, Yin, Chao, Wang, Yaonan, Geng, Haopeng, Pan, Zhiming and Jiao, Xinan (2017) Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model. Microbial Pathogenesis, 104 . pp. 146-150. ISSN 1096-1208 Salmonella Pullorum ; Signature-tagged mutagenesis ; Chicken embryo http://www.sciencedirect.com/science/article/pii/S0882401016302704?via%3Dihub doi:10.1016/j.micpath.2017.01.014 doi:10.1016/j.micpath.2017.01.014 |
| spellingShingle | Salmonella Pullorum ; Signature-tagged mutagenesis ; Chicken embryo Geng, Shizhong Tian, Qin Guo, Rongxian Jiao, Yang Barrow, Paul Yin, Chao Wang, Yaonan Geng, Haopeng Pan, Zhiming Jiao, Xinan Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model |
| title | Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model |
| title_full | Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model |
| title_fullStr | Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model |
| title_full_unstemmed | Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model |
| title_short | Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model |
| title_sort | identification by pcr signature-tagged mutagenesis of attenuated salmonella pullorum mutants and corresponding genes in a chicken embryo model |
| topic | Salmonella Pullorum ; Signature-tagged mutagenesis ; Chicken embryo |
| url | https://eprints.nottingham.ac.uk/45099/ https://eprints.nottingham.ac.uk/45099/ https://eprints.nottingham.ac.uk/45099/ |