Experimental infection of chickens by a flagellated motile strain of Salmonella enterica serovar Gallinarum biovar Gallinarum
Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum (SG) causes fowl typhoid (FT), a septicaemic disease which can result in high mortality in poultry flocks. The absence of flagella in SG is thought to favour systemic invasion, since bacterial recognition via Toll-like receptor...
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Elsevier
2016
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| Online Access: | https://eprints.nottingham.ac.uk/45096/ |
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| author | Lopes, P.D. Freitas Neto, O.C. Batista, D.F.A. Denadai, J. Alarcon, M.F.F. Almeida, A.M. Vasconcelos, R.O. Setta, A. Barrow, P.A. Berchieri, A. |
| author_facet | Lopes, P.D. Freitas Neto, O.C. Batista, D.F.A. Denadai, J. Alarcon, M.F.F. Almeida, A.M. Vasconcelos, R.O. Setta, A. Barrow, P.A. Berchieri, A. |
| author_sort | Lopes, P.D. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum (SG) causes fowl typhoid (FT), a septicaemic disease which can result in high mortality in poultry flocks. The absence of flagella in SG is thought to favour systemic invasion, since bacterial recognition via Toll-like receptor (TLR)-5 does not take place during the early stages of FT. In the present study, chicks susceptible to FT were inoculated with a wild type SG (SG) or its flagellated motile derivative (SG Fla+). In experiment 1, mortality and clinical signs were assessed, whereas in experiment 2, gross pathology, histopathology, systemic invasion and immune responses were evaluated. SG Fla+ infection resulted in later development of clinical signs, lower mortality, lower bacterial numbers in the liver and spleen, and less severe pathological changes compared to SG. The CD8+ T lymphocyte population was higher in the livers of chicks infected with SG at 4 days post-inoculation (dpi). Chicks infected with SG had increased expression of interleukin (IL)-6 mRNA in the caecal tonsil at 1 dpi and increased expression of IL-18 mRNA in the spleen at 4 dpi. In contrast, the CD4+ T lymphocyte population was higher at 6 dpi in the livers of birds infected with SG Fla+. Therefore, flagella appeared to modulate the chicken immune response towards a CD4+ T profile, resulting in more efficient bacterial clearance from systemic sites and milder infection. |
| first_indexed | 2025-11-14T19:58:00Z |
| format | Article |
| id | nottingham-45096 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:58:00Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-450962020-05-04T17:58:33Z https://eprints.nottingham.ac.uk/45096/ Experimental infection of chickens by a flagellated motile strain of Salmonella enterica serovar Gallinarum biovar Gallinarum Lopes, P.D. Freitas Neto, O.C. Batista, D.F.A. Denadai, J. Alarcon, M.F.F. Almeida, A.M. Vasconcelos, R.O. Setta, A. Barrow, P.A. Berchieri, A. Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum (SG) causes fowl typhoid (FT), a septicaemic disease which can result in high mortality in poultry flocks. The absence of flagella in SG is thought to favour systemic invasion, since bacterial recognition via Toll-like receptor (TLR)-5 does not take place during the early stages of FT. In the present study, chicks susceptible to FT were inoculated with a wild type SG (SG) or its flagellated motile derivative (SG Fla+). In experiment 1, mortality and clinical signs were assessed, whereas in experiment 2, gross pathology, histopathology, systemic invasion and immune responses were evaluated. SG Fla+ infection resulted in later development of clinical signs, lower mortality, lower bacterial numbers in the liver and spleen, and less severe pathological changes compared to SG. The CD8+ T lymphocyte population was higher in the livers of chicks infected with SG at 4 days post-inoculation (dpi). Chicks infected with SG had increased expression of interleukin (IL)-6 mRNA in the caecal tonsil at 1 dpi and increased expression of IL-18 mRNA in the spleen at 4 dpi. In contrast, the CD4+ T lymphocyte population was higher at 6 dpi in the livers of birds infected with SG Fla+. Therefore, flagella appeared to modulate the chicken immune response towards a CD4+ T profile, resulting in more efficient bacterial clearance from systemic sites and milder infection. Elsevier 2016-08-01 Article PeerReviewed Lopes, P.D., Freitas Neto, O.C., Batista, D.F.A., Denadai, J., Alarcon, M.F.F., Almeida, A.M., Vasconcelos, R.O., Setta, A., Barrow, P.A. and Berchieri, A. (2016) Experimental infection of chickens by a flagellated motile strain of Salmonella enterica serovar Gallinarum biovar Gallinarum. Veterinary Journal, 214 . pp. 40-46. ISSN 1090-0233 Domestic fowl ; Fowl typhoid ; Salmonella enterica biovar Gallinarum ; Pathogenicity ; Immune response http://www.sciencedirect.com/science/article/pii/S1090023316300442?via%3Dihub doi:10.1016/j.tvjl.2016.05.006 doi:10.1016/j.tvjl.2016.05.006 |
| spellingShingle | Domestic fowl ; Fowl typhoid ; Salmonella enterica biovar Gallinarum ; Pathogenicity ; Immune response Lopes, P.D. Freitas Neto, O.C. Batista, D.F.A. Denadai, J. Alarcon, M.F.F. Almeida, A.M. Vasconcelos, R.O. Setta, A. Barrow, P.A. Berchieri, A. Experimental infection of chickens by a flagellated motile strain of Salmonella enterica serovar Gallinarum biovar Gallinarum |
| title | Experimental infection of chickens by a flagellated motile strain of Salmonella enterica serovar Gallinarum biovar Gallinarum |
| title_full | Experimental infection of chickens by a flagellated motile strain of Salmonella enterica serovar Gallinarum biovar Gallinarum |
| title_fullStr | Experimental infection of chickens by a flagellated motile strain of Salmonella enterica serovar Gallinarum biovar Gallinarum |
| title_full_unstemmed | Experimental infection of chickens by a flagellated motile strain of Salmonella enterica serovar Gallinarum biovar Gallinarum |
| title_short | Experimental infection of chickens by a flagellated motile strain of Salmonella enterica serovar Gallinarum biovar Gallinarum |
| title_sort | experimental infection of chickens by a flagellated motile strain of salmonella enterica serovar gallinarum biovar gallinarum |
| topic | Domestic fowl ; Fowl typhoid ; Salmonella enterica biovar Gallinarum ; Pathogenicity ; Immune response |
| url | https://eprints.nottingham.ac.uk/45096/ https://eprints.nottingham.ac.uk/45096/ https://eprints.nottingham.ac.uk/45096/ |