A structural study of Acacia nilotica and Acacia modesta gums

Superficially similar carbohydrate polymers from similar sources can have dramatically different characteristics. This work seeks to examine the molecular properties responsible for these differences. Protons responsible for cross-polarization in the anomeric region of Acacia nilotica (AN) were repl...

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Main Authors: Massey, Shazma, MacNaughtan, William, Williams, Huw E.L., Wolf, Bettina, Iqbal, Mohammad S.
Format: Article
Published: Elsevier 2017
Subjects:
Online Access:https://eprints.nottingham.ac.uk/44902/
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author Massey, Shazma
MacNaughtan, William
Williams, Huw E.L.
Wolf, Bettina
Iqbal, Mohammad S.
author_facet Massey, Shazma
MacNaughtan, William
Williams, Huw E.L.
Wolf, Bettina
Iqbal, Mohammad S.
author_sort Massey, Shazma
building Nottingham Research Data Repository
collection Online Access
description Superficially similar carbohydrate polymers from similar sources can have dramatically different characteristics. This work seeks to examine the molecular properties responsible for these differences. Protons responsible for cross-polarization in the anomeric region of Acacia nilotica (AN) were replaced easily by deuterium, but not for Acacia modesta (AM). Time constants describing the mobility and cross-polarization transfer were both found to be lower for AM. Variable contact time experiments showed poorer fits and more heterogeneity for AN. Solution state HSQC experiments showed a lower number of environments in the anomeric region for AM. The relaxation time T2 of AM solutions had a lower value consistent with a higher viscosity. The Tg′ of solutions were −14.5 °C AN and −18.5 °C AM. These results form a largely self-consistent picture of molecular differences between AN and AM, suggesting a more compact but heterogeneous structure for AN and more branching in the case of AM.
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spelling nottingham-449022020-05-04T19:15:46Z https://eprints.nottingham.ac.uk/44902/ A structural study of Acacia nilotica and Acacia modesta gums Massey, Shazma MacNaughtan, William Williams, Huw E.L. Wolf, Bettina Iqbal, Mohammad S. Superficially similar carbohydrate polymers from similar sources can have dramatically different characteristics. This work seeks to examine the molecular properties responsible for these differences. Protons responsible for cross-polarization in the anomeric region of Acacia nilotica (AN) were replaced easily by deuterium, but not for Acacia modesta (AM). Time constants describing the mobility and cross-polarization transfer were both found to be lower for AM. Variable contact time experiments showed poorer fits and more heterogeneity for AN. Solution state HSQC experiments showed a lower number of environments in the anomeric region for AM. The relaxation time T2 of AM solutions had a lower value consistent with a higher viscosity. The Tg′ of solutions were −14.5 °C AN and −18.5 °C AM. These results form a largely self-consistent picture of molecular differences between AN and AM, suggesting a more compact but heterogeneous structure for AN and more branching in the case of AM. Elsevier 2017-11-01 Article PeerReviewed Massey, Shazma, MacNaughtan, William, Williams, Huw E.L., Wolf, Bettina and Iqbal, Mohammad S. (2017) A structural study of Acacia nilotica and Acacia modesta gums. Carbohydrate Polymers, 175 . pp. 207-215. ISSN 1879-1344 Arabinans; Acacia modesta; Acacia nilotica; Polysaccharides; NMR spectroscopy http://www.sciencedirect.com/science/article/pii/S014486171730841X doi:10.1016/j.carbpol.2017.07.065 doi:10.1016/j.carbpol.2017.07.065
spellingShingle Arabinans; Acacia modesta; Acacia nilotica; Polysaccharides; NMR spectroscopy
Massey, Shazma
MacNaughtan, William
Williams, Huw E.L.
Wolf, Bettina
Iqbal, Mohammad S.
A structural study of Acacia nilotica and Acacia modesta gums
title A structural study of Acacia nilotica and Acacia modesta gums
title_full A structural study of Acacia nilotica and Acacia modesta gums
title_fullStr A structural study of Acacia nilotica and Acacia modesta gums
title_full_unstemmed A structural study of Acacia nilotica and Acacia modesta gums
title_short A structural study of Acacia nilotica and Acacia modesta gums
title_sort structural study of acacia nilotica and acacia modesta gums
topic Arabinans; Acacia modesta; Acacia nilotica; Polysaccharides; NMR spectroscopy
url https://eprints.nottingham.ac.uk/44902/
https://eprints.nottingham.ac.uk/44902/
https://eprints.nottingham.ac.uk/44902/