Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells

Naturally occurring extracellular vesicles (EVs) play essential roles in intracellular communication and delivery of bioactive molecules. Therefore it has been suggested that EVs could be used for delivery of therapeutics. However, to date the therapeutic application of EVs has been limited by numbe...

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Main Authors: Gomzikova, Marina, Zhuravleva, Margarita, Miftakhova, Regina, Arkhipova, Svetlana, Evtugin, Vladimir, Khaiboullina, Sventlana, Kiyasov, Andrey, Persson, Jenny, Mongan, Nigel P., Pestell, Richard, Rizvanov, Albert A.
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Published: Impact Journals 2017
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Online Access:https://eprints.nottingham.ac.uk/44621/
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author Gomzikova, Marina
Zhuravleva, Margarita
Miftakhova, Regina
Arkhipova, Svetlana
Evtugin, Vladimir
Khaiboullina, Sventlana
Kiyasov, Andrey
Persson, Jenny
Mongan, Nigel P.
Pestell, Richard
Rizvanov, Albert A.
author_facet Gomzikova, Marina
Zhuravleva, Margarita
Miftakhova, Regina
Arkhipova, Svetlana
Evtugin, Vladimir
Khaiboullina, Sventlana
Kiyasov, Andrey
Persson, Jenny
Mongan, Nigel P.
Pestell, Richard
Rizvanov, Albert A.
author_sort Gomzikova, Marina
building Nottingham Research Data Repository
collection Online Access
description Naturally occurring extracellular vesicles (EVs) play essential roles in intracellular communication and delivery of bioactive molecules. Therefore it has been suggested that EVs could be used for delivery of therapeutics. However, to date the therapeutic application of EVs has been limited by number of factors, including limited yield and full understanding of their biological activities. To address these issues, we analyzed the morphology, molecular composition, fusion capacity and biological activity of Cytochalasin B-induced membrane vesicles (CIMVs). The size of these vesicles was comparable to that of naturally occurring EVs. In addition, we have shown that CIMVs from human SH-SY5Y cells contain elevated levels of VEGF as compared to the parental cells, and stimulate angiogenesis in vitro and in vivo.
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spelling nottingham-446212020-05-04T18:57:42Z https://eprints.nottingham.ac.uk/44621/ Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells Gomzikova, Marina Zhuravleva, Margarita Miftakhova, Regina Arkhipova, Svetlana Evtugin, Vladimir Khaiboullina, Sventlana Kiyasov, Andrey Persson, Jenny Mongan, Nigel P. Pestell, Richard Rizvanov, Albert A. Naturally occurring extracellular vesicles (EVs) play essential roles in intracellular communication and delivery of bioactive molecules. Therefore it has been suggested that EVs could be used for delivery of therapeutics. However, to date the therapeutic application of EVs has been limited by number of factors, including limited yield and full understanding of their biological activities. To address these issues, we analyzed the morphology, molecular composition, fusion capacity and biological activity of Cytochalasin B-induced membrane vesicles (CIMVs). The size of these vesicles was comparable to that of naturally occurring EVs. In addition, we have shown that CIMVs from human SH-SY5Y cells contain elevated levels of VEGF as compared to the parental cells, and stimulate angiogenesis in vitro and in vivo. Impact Journals 2017-07-31 Article PeerReviewed Gomzikova, Marina, Zhuravleva, Margarita, Miftakhova, Regina, Arkhipova, Svetlana, Evtugin, Vladimir, Khaiboullina, Sventlana, Kiyasov, Andrey, Persson, Jenny, Mongan, Nigel P., Pestell, Richard and Rizvanov, Albert A. (2017) Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells. Oncotarget, 8 (41). pp. 70496-70507. ISSN 1949-2553 extracellular vesicles membrane vesicles Cytochalasin B-induced membrane vesicles angiogenesis cell-free therapy http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=19723&path[]=63002 doi:10.18632/oncotarget.19723 doi:10.18632/oncotarget.19723
spellingShingle extracellular vesicles
membrane vesicles
Cytochalasin B-induced membrane vesicles
angiogenesis
cell-free therapy
Gomzikova, Marina
Zhuravleva, Margarita
Miftakhova, Regina
Arkhipova, Svetlana
Evtugin, Vladimir
Khaiboullina, Sventlana
Kiyasov, Andrey
Persson, Jenny
Mongan, Nigel P.
Pestell, Richard
Rizvanov, Albert A.
Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells
title Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells
title_full Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells
title_fullStr Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells
title_full_unstemmed Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells
title_short Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells
title_sort cytochalasin b-induced membrane vesicles convey angiogenic activity of parental cells
topic extracellular vesicles
membrane vesicles
Cytochalasin B-induced membrane vesicles
angiogenesis
cell-free therapy
url https://eprints.nottingham.ac.uk/44621/
https://eprints.nottingham.ac.uk/44621/
https://eprints.nottingham.ac.uk/44621/