Expression of NAD(P)H quinone dehydrogenase 1 (NQO1) is increased in the endometrium of women with endometrial cancer and women with Polycystic Ovary Syndrome
OBJECTIVE: Women with a prior history of polycystic ovary syndrome (PCOS) have an increased risk of endometrial cancer (EC). AIM: To investigate whether the endometrium of women with PCOS possess gene expression changes similar to those found in EC. DESIGN AND METHODS: Patients with EC, PCOS and c...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
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Wiley
2017
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| Online Access: | https://eprints.nottingham.ac.uk/44499/ |
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| author | Atiomo, William Shafiee, Mohamad Nasir Chapman, Caroline Metzler, Veronika M. Abouzeid, Jad Latif, Ayşe Chadwick, Amy Kitson, Sarah Sivalingam, Vanitha N. Stratford, Ian J Rutland, Catrin S. Persson, Jenny L. Ødum, Niels Fuentes-Utrillia, Pablo Jeyapalan, Jennie N. Heery, David M. Crosbie, Emma J. Mongan, Nigel P. |
| author_facet | Atiomo, William Shafiee, Mohamad Nasir Chapman, Caroline Metzler, Veronika M. Abouzeid, Jad Latif, Ayşe Chadwick, Amy Kitson, Sarah Sivalingam, Vanitha N. Stratford, Ian J Rutland, Catrin S. Persson, Jenny L. Ødum, Niels Fuentes-Utrillia, Pablo Jeyapalan, Jennie N. Heery, David M. Crosbie, Emma J. Mongan, Nigel P. |
| author_sort | Atiomo, William |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | OBJECTIVE: Women with a prior history of polycystic ovary syndrome (PCOS) have an increased risk of endometrial cancer (EC).
AIM: To investigate whether the endometrium of women with PCOS possess gene expression changes similar to those found in EC.
DESIGN AND METHODS: Patients with EC, PCOS and control women unaffected by either PCOS or EC were recruited into a cross-sectional study at the Nottingham University Hospital, UK. For RNA sequencing, representative individual endometrial biopsies were obtained from women with EC, PCOS and a woman unaffected by PCOS or EC. Expression of a subset of differentially expressed genes identified by RNA sequencing, including NAD(P)H quinone dehydrogenase 1 (NQO1), were validated by quantitative reverse transcriptase PCR validation (n=76) and in the cancer genome atlas UCEC (uterine corpus endometrioid carcinoma) RNA sequencing dataset (n=381). The expression of NQO1 was validated by immuno-histochemistry in EC samples from a separate cohort (n=91) comprised of consecutive patients who underwent hysterectomy at St Mary's Hospital, Manchester between 2011 and 2013. A further 6 postmenopausal women with histologically normal endometrium who underwent hysterectomy for genital prolapse were also included. Informed consent and local ethics approval was obtained for the study.
RESULTS: We show for the first that that NQO1 expression is significantly increased in the endometrium of women with PCOS and EC. Immunohistochemistry confirms significantly increased NQO1 protein expression in EC relative to non-malignant endometrial tissue (p<0.0001).
CONCLUSIONS: The results obtained here support a previously unrecognized molecular link between PCOS and EC involving NQO1. |
| first_indexed | 2025-11-14T19:55:48Z |
| format | Article |
| id | nottingham-44499 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:55:48Z |
| publishDate | 2017 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-444992024-08-15T15:23:24Z https://eprints.nottingham.ac.uk/44499/ Expression of NAD(P)H quinone dehydrogenase 1 (NQO1) is increased in the endometrium of women with endometrial cancer and women with Polycystic Ovary Syndrome Atiomo, William Shafiee, Mohamad Nasir Chapman, Caroline Metzler, Veronika M. Abouzeid, Jad Latif, Ayşe Chadwick, Amy Kitson, Sarah Sivalingam, Vanitha N. Stratford, Ian J Rutland, Catrin S. Persson, Jenny L. Ødum, Niels Fuentes-Utrillia, Pablo Jeyapalan, Jennie N. Heery, David M. Crosbie, Emma J. Mongan, Nigel P. OBJECTIVE: Women with a prior history of polycystic ovary syndrome (PCOS) have an increased risk of endometrial cancer (EC). AIM: To investigate whether the endometrium of women with PCOS possess gene expression changes similar to those found in EC. DESIGN AND METHODS: Patients with EC, PCOS and control women unaffected by either PCOS or EC were recruited into a cross-sectional study at the Nottingham University Hospital, UK. For RNA sequencing, representative individual endometrial biopsies were obtained from women with EC, PCOS and a woman unaffected by PCOS or EC. Expression of a subset of differentially expressed genes identified by RNA sequencing, including NAD(P)H quinone dehydrogenase 1 (NQO1), were validated by quantitative reverse transcriptase PCR validation (n=76) and in the cancer genome atlas UCEC (uterine corpus endometrioid carcinoma) RNA sequencing dataset (n=381). The expression of NQO1 was validated by immuno-histochemistry in EC samples from a separate cohort (n=91) comprised of consecutive patients who underwent hysterectomy at St Mary's Hospital, Manchester between 2011 and 2013. A further 6 postmenopausal women with histologically normal endometrium who underwent hysterectomy for genital prolapse were also included. Informed consent and local ethics approval was obtained for the study. RESULTS: We show for the first that that NQO1 expression is significantly increased in the endometrium of women with PCOS and EC. Immunohistochemistry confirms significantly increased NQO1 protein expression in EC relative to non-malignant endometrial tissue (p<0.0001). CONCLUSIONS: The results obtained here support a previously unrecognized molecular link between PCOS and EC involving NQO1. Wiley 2017-07-27 Article PeerReviewed Atiomo, William, Shafiee, Mohamad Nasir, Chapman, Caroline, Metzler, Veronika M., Abouzeid, Jad, Latif, Ayşe, Chadwick, Amy, Kitson, Sarah, Sivalingam, Vanitha N., Stratford, Ian J, Rutland, Catrin S., Persson, Jenny L., Ødum, Niels, Fuentes-Utrillia, Pablo, Jeyapalan, Jennie N., Heery, David M., Crosbie, Emma J. and Mongan, Nigel P. (2017) Expression of NAD(P)H quinone dehydrogenase 1 (NQO1) is increased in the endometrium of women with endometrial cancer and women with Polycystic Ovary Syndrome. Clinical Endocrinology, 87 (5). pp. 557-565. ISSN 1365-2265 Endometrial cancer endometrium polycystic ovary syndrome NQO1 http://onlinelibrary.wiley.com/doi/10.1111/cen.13436/abstract doi:10.1111/cen.13436 doi:10.1111/cen.13436 |
| spellingShingle | Endometrial cancer endometrium polycystic ovary syndrome NQO1 Atiomo, William Shafiee, Mohamad Nasir Chapman, Caroline Metzler, Veronika M. Abouzeid, Jad Latif, Ayşe Chadwick, Amy Kitson, Sarah Sivalingam, Vanitha N. Stratford, Ian J Rutland, Catrin S. Persson, Jenny L. Ødum, Niels Fuentes-Utrillia, Pablo Jeyapalan, Jennie N. Heery, David M. Crosbie, Emma J. Mongan, Nigel P. Expression of NAD(P)H quinone dehydrogenase 1 (NQO1) is increased in the endometrium of women with endometrial cancer and women with Polycystic Ovary Syndrome |
| title | Expression of NAD(P)H quinone dehydrogenase 1 (NQO1) is increased in the endometrium of women with endometrial cancer and women with Polycystic Ovary Syndrome |
| title_full | Expression of NAD(P)H quinone dehydrogenase 1 (NQO1) is increased in the endometrium of women with endometrial cancer and women with Polycystic Ovary Syndrome |
| title_fullStr | Expression of NAD(P)H quinone dehydrogenase 1 (NQO1) is increased in the endometrium of women with endometrial cancer and women with Polycystic Ovary Syndrome |
| title_full_unstemmed | Expression of NAD(P)H quinone dehydrogenase 1 (NQO1) is increased in the endometrium of women with endometrial cancer and women with Polycystic Ovary Syndrome |
| title_short | Expression of NAD(P)H quinone dehydrogenase 1 (NQO1) is increased in the endometrium of women with endometrial cancer and women with Polycystic Ovary Syndrome |
| title_sort | expression of nad(p)h quinone dehydrogenase 1 (nqo1) is increased in the endometrium of women with endometrial cancer and women with polycystic ovary syndrome |
| topic | Endometrial cancer endometrium polycystic ovary syndrome NQO1 |
| url | https://eprints.nottingham.ac.uk/44499/ https://eprints.nottingham.ac.uk/44499/ https://eprints.nottingham.ac.uk/44499/ |