Dynamic regulation of auxin response during rice development revealed by newly established hormone biosensor markers
The hormone auxin is critical for many plant developmental processes. Unlike the model eudicot plant Arabidopsis (Arabidopsis thaliana), auxin distribution and signaling in rice tissues has not been systematically investigated due to the absence of suitable auxin response reporters. In this study we...
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Frontiers Media
2017
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| Online Access: | https://eprints.nottingham.ac.uk/44429/ |
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| author | Yang, Jing Yuan, Zheng Meng, Qingcai Huang, Guoqiang Périn, Christophe Bureau, Charlotte Meunier, Anne-Cécile Ingouff, Mathieu Bennett, Malcolm J. Liang, Wanqi Zhang, Dabing |
| author_facet | Yang, Jing Yuan, Zheng Meng, Qingcai Huang, Guoqiang Périn, Christophe Bureau, Charlotte Meunier, Anne-Cécile Ingouff, Mathieu Bennett, Malcolm J. Liang, Wanqi Zhang, Dabing |
| author_sort | Yang, Jing |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The hormone auxin is critical for many plant developmental processes. Unlike the model eudicot plant Arabidopsis (Arabidopsis thaliana), auxin distribution and signaling in rice tissues has not been systematically investigated due to the absence of suitable auxin response reporters. In this study we observed the conservation of auxin signalling components between Arabidopsis and model monocot crop rice (Oryza sativa), and generated complementary types of auxin biosensor constructs, one derived from the Aux/IAA-based biosensor DII-VENUS but constitutively driven by maize ubiquitin-1 promoter, and the other termed DR5-VENUS in which a synthetic auxin-responsive promoter (DR5rev) was used to drive expression of the yellow fluorescent protein (YFP). Using the obtained transgenic lines, we observed that during the vegetative development, accumulation of DR5-VENUS signal was at young and mature leaves, tiller buds and stem base. Notably, abundant DR5-VENUS signals were observed in the cytoplasm of cortex cells surrounding lateral root primordia (LRP) in rice. In addition, auxinmaxima and dynamic re-localization were seen at the initiation sites of inflorescence and spikelet primordia including branch meristems (BMs), female and male organs. The comparison of these observations among Arabidopsis, rice and maize suggests the unique role of auxin in regulating rice lateral root emergence and reproduction. Moreover, protein localization of auxin transporters PIN1 homologs and GFP tagged OsAUX1 overlapped with DR5-VENUS during spikelet development, helping validate these auxin response reporters are reliable markers in rice. This work firstly reveals the direct correspondence between auxin distribution and rice reproductive and root development at tissue and cellular level, and provides high-resolution auxin tools to probe fundamental developmental processes in rice and to establish links between auxin, development and agronomical traits like yield or root architecture. |
| first_indexed | 2025-11-14T19:55:34Z |
| format | Article |
| id | nottingham-44429 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:55:34Z |
| publishDate | 2017 |
| publisher | Frontiers Media |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-444292020-05-04T18:36:46Z https://eprints.nottingham.ac.uk/44429/ Dynamic regulation of auxin response during rice development revealed by newly established hormone biosensor markers Yang, Jing Yuan, Zheng Meng, Qingcai Huang, Guoqiang Périn, Christophe Bureau, Charlotte Meunier, Anne-Cécile Ingouff, Mathieu Bennett, Malcolm J. Liang, Wanqi Zhang, Dabing The hormone auxin is critical for many plant developmental processes. Unlike the model eudicot plant Arabidopsis (Arabidopsis thaliana), auxin distribution and signaling in rice tissues has not been systematically investigated due to the absence of suitable auxin response reporters. In this study we observed the conservation of auxin signalling components between Arabidopsis and model monocot crop rice (Oryza sativa), and generated complementary types of auxin biosensor constructs, one derived from the Aux/IAA-based biosensor DII-VENUS but constitutively driven by maize ubiquitin-1 promoter, and the other termed DR5-VENUS in which a synthetic auxin-responsive promoter (DR5rev) was used to drive expression of the yellow fluorescent protein (YFP). Using the obtained transgenic lines, we observed that during the vegetative development, accumulation of DR5-VENUS signal was at young and mature leaves, tiller buds and stem base. Notably, abundant DR5-VENUS signals were observed in the cytoplasm of cortex cells surrounding lateral root primordia (LRP) in rice. In addition, auxinmaxima and dynamic re-localization were seen at the initiation sites of inflorescence and spikelet primordia including branch meristems (BMs), female and male organs. The comparison of these observations among Arabidopsis, rice and maize suggests the unique role of auxin in regulating rice lateral root emergence and reproduction. Moreover, protein localization of auxin transporters PIN1 homologs and GFP tagged OsAUX1 overlapped with DR5-VENUS during spikelet development, helping validate these auxin response reporters are reliable markers in rice. This work firstly reveals the direct correspondence between auxin distribution and rice reproductive and root development at tissue and cellular level, and provides high-resolution auxin tools to probe fundamental developmental processes in rice and to establish links between auxin, development and agronomical traits like yield or root architecture. Frontiers Media 2017-03-07 Article PeerReviewed Yang, Jing, Yuan, Zheng, Meng, Qingcai, Huang, Guoqiang, Périn, Christophe, Bureau, Charlotte, Meunier, Anne-Cécile, Ingouff, Mathieu, Bennett, Malcolm J., Liang, Wanqi and Zhang, Dabing (2017) Dynamic regulation of auxin response during rice development revealed by newly established hormone biosensor markers. Frontiers in Plant Science, 8 . p. 256. ISSN 1664-462X rice auxin reporter lateral root formation inflorescence spikelet meristem http://journal.frontiersin.org/article/10.3389/fpls.2017.00256/full doi:10.3389/fpls.2017.00256 doi:10.3389/fpls.2017.00256 |
| spellingShingle | rice auxin reporter lateral root formation inflorescence spikelet meristem Yang, Jing Yuan, Zheng Meng, Qingcai Huang, Guoqiang Périn, Christophe Bureau, Charlotte Meunier, Anne-Cécile Ingouff, Mathieu Bennett, Malcolm J. Liang, Wanqi Zhang, Dabing Dynamic regulation of auxin response during rice development revealed by newly established hormone biosensor markers |
| title | Dynamic regulation of auxin response during rice development revealed by newly established hormone biosensor markers |
| title_full | Dynamic regulation of auxin response during rice development revealed by newly established hormone biosensor markers |
| title_fullStr | Dynamic regulation of auxin response during rice development revealed by newly established hormone biosensor markers |
| title_full_unstemmed | Dynamic regulation of auxin response during rice development revealed by newly established hormone biosensor markers |
| title_short | Dynamic regulation of auxin response during rice development revealed by newly established hormone biosensor markers |
| title_sort | dynamic regulation of auxin response during rice development revealed by newly established hormone biosensor markers |
| topic | rice auxin reporter lateral root formation inflorescence spikelet meristem |
| url | https://eprints.nottingham.ac.uk/44429/ https://eprints.nottingham.ac.uk/44429/ https://eprints.nottingham.ac.uk/44429/ |