Tissue cell stress response to obesity and its interaction with late gestational diet

Intra-uterine growth restriction in late pregnancy can contribute to adverse long term metabolic health in the offspring. We utilised an animal (sheep) model of maternal dietary manipulation in late pregnancy, combined with exposure of the offspring to a low activity, obesogenic environment after we...

Full description

Bibliographic Details
Main Authors: Saroha, Vivek, Dellschaft, Neele S., Keisler, Duane H., Gardner, David S., Budge, Helen, Sebert, Sylvain P., Symonds, Michael E.
Format: Article
Published: CSIRO Publishing 2018
Subjects:
Online Access:https://eprints.nottingham.ac.uk/44428/
_version_ 1848796915323371520
author Saroha, Vivek
Dellschaft, Neele S.
Keisler, Duane H.
Gardner, David S.
Budge, Helen
Sebert, Sylvain P.
Symonds, Michael E.
author_facet Saroha, Vivek
Dellschaft, Neele S.
Keisler, Duane H.
Gardner, David S.
Budge, Helen
Sebert, Sylvain P.
Symonds, Michael E.
author_sort Saroha, Vivek
building Nottingham Research Data Repository
collection Online Access
description Intra-uterine growth restriction in late pregnancy can contribute to adverse long term metabolic health in the offspring. We utilised an animal (sheep) model of maternal dietary manipulation in late pregnancy, combined with exposure of the offspring to a low activity, obesogenic environment after weaning, to characterise the effects on glucose homeostasis. Dizygotic twin-pregnant sheep were either fed to 60% of requirements (nutrient restriction (R)) or fed ad libitum (~ 140% of requirements (A)) from 110 days gestation until term (~147d). After weaning (~3 months of age), their offspring were kept in either a standard (in order to remain lean) or low activity, obesogenic environment. R mothers gained less weight and produced smaller offspring. As adults, obese offspring were heavier and fatter with reduced glucose tolerance, irrespective of maternal diet. Molecular markers of stress and autophagy in liver and adipose tissue were increased with obesity, with gene expression of hepatic Grp78 and of omental Atf6, Grp78 and Edem1 only being increased in R offspring. In conclusion, the adverse effect of juvenile onset obesity on insulin responsive tissues can be amplified by previous exposure to a suboptimal nutritional environment in utero, thereby contributing to earlier onset of insulin resistance.
first_indexed 2025-11-14T19:55:34Z
format Article
id nottingham-44428
institution University of Nottingham Malaysia Campus
institution_category Local University
last_indexed 2025-11-14T19:55:34Z
publishDate 2018
publisher CSIRO Publishing
recordtype eprints
repository_type Digital Repository
spelling nottingham-444282020-05-04T19:29:31Z https://eprints.nottingham.ac.uk/44428/ Tissue cell stress response to obesity and its interaction with late gestational diet Saroha, Vivek Dellschaft, Neele S. Keisler, Duane H. Gardner, David S. Budge, Helen Sebert, Sylvain P. Symonds, Michael E. Intra-uterine growth restriction in late pregnancy can contribute to adverse long term metabolic health in the offspring. We utilised an animal (sheep) model of maternal dietary manipulation in late pregnancy, combined with exposure of the offspring to a low activity, obesogenic environment after weaning, to characterise the effects on glucose homeostasis. Dizygotic twin-pregnant sheep were either fed to 60% of requirements (nutrient restriction (R)) or fed ad libitum (~ 140% of requirements (A)) from 110 days gestation until term (~147d). After weaning (~3 months of age), their offspring were kept in either a standard (in order to remain lean) or low activity, obesogenic environment. R mothers gained less weight and produced smaller offspring. As adults, obese offspring were heavier and fatter with reduced glucose tolerance, irrespective of maternal diet. Molecular markers of stress and autophagy in liver and adipose tissue were increased with obesity, with gene expression of hepatic Grp78 and of omental Atf6, Grp78 and Edem1 only being increased in R offspring. In conclusion, the adverse effect of juvenile onset obesity on insulin responsive tissues can be amplified by previous exposure to a suboptimal nutritional environment in utero, thereby contributing to earlier onset of insulin resistance. CSIRO Publishing 2018-01-30 Article PeerReviewed Saroha, Vivek, Dellschaft, Neele S., Keisler, Duane H., Gardner, David S., Budge, Helen, Sebert, Sylvain P. and Symonds, Michael E. (2018) Tissue cell stress response to obesity and its interaction with late gestational diet. Reproduction, Fertility and Development, 30 . pp. 430-441. ISSN 1448-5990 adipose tissue appetite growth nutrition http://www.publish.csiro.au/rd/RD16494 doi:10.1071/RD16494 doi:10.1071/RD16494
spellingShingle adipose tissue
appetite
growth
nutrition
Saroha, Vivek
Dellschaft, Neele S.
Keisler, Duane H.
Gardner, David S.
Budge, Helen
Sebert, Sylvain P.
Symonds, Michael E.
Tissue cell stress response to obesity and its interaction with late gestational diet
title Tissue cell stress response to obesity and its interaction with late gestational diet
title_full Tissue cell stress response to obesity and its interaction with late gestational diet
title_fullStr Tissue cell stress response to obesity and its interaction with late gestational diet
title_full_unstemmed Tissue cell stress response to obesity and its interaction with late gestational diet
title_short Tissue cell stress response to obesity and its interaction with late gestational diet
title_sort tissue cell stress response to obesity and its interaction with late gestational diet
topic adipose tissue
appetite
growth
nutrition
url https://eprints.nottingham.ac.uk/44428/
https://eprints.nottingham.ac.uk/44428/
https://eprints.nottingham.ac.uk/44428/