A scanner-based rhizobox system enabling the quantification of root system development and response of Brassica rapa seedlings to external P availability
Rhizoboxes are soil-root compartments that may well provide the closest naturalistic conditions for studying root systems architectures (RSAs) in controlled environments. Rhizobox-based studies can however lead to mis-estimation of root traits due to poor recovery of roots and loss of fine root feat...
| Main Authors: | , , , , , |
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| Format: | Article |
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Japanese Society for Root Research
2017
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| Online Access: | https://eprints.nottingham.ac.uk/44350/ |
| _version_ | 1848796896991117312 |
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| author | Adu, Michael Osei Yawson, David O. Bennett, Malcolm J. Broadley, Martin R. Dupuy, Lionel X. White, Philip J. |
| author_facet | Adu, Michael Osei Yawson, David O. Bennett, Malcolm J. Broadley, Martin R. Dupuy, Lionel X. White, Philip J. |
| author_sort | Adu, Michael Osei |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Rhizoboxes are soil-root compartments that may well provide the closest naturalistic conditions for studying root systems architectures (RSAs) in controlled environments. Rhizobox-based studies can however lead to mis-estimation of root traits due to poor recovery of roots and loss of fine root features during washing of roots. We used a novel scanner-based rhizobox system to evaluate: (i) RSA traits of Brassica rapa genotypes; (ii) the relationship between root traits recorded from rhizoboxes and those of harvested roots and (iii) genotypic variation of seedlings in response to external P ([P] ext) availability. Brassica rapa genotypes were grown in soil-filled rhizoboxes abutting flatbed scanners and were watered once with either deionised water or a solution of 600 μM KH2PO4 to approximately 80% field capacity on a weight basis. Shoot and root P concentrations ([P]shoot and [P]root) of the B. rapa lines grown on different [P]ext were quantified. Visible root length at the surface of rhizoboxes constituted 85% of the total root length recovered from harvested root samples. High P supply induced a strong increase in [P]shoot in all genotypes (P < 0.001) whereas low P supply generally led to greater partitioning to roots. Seed P concentration and tissue P concentration were correlated only at low [P]ext. Total root length was strongly correlated with tissue P content under both low [P]ext (r = 0.81, P < 0.05) and high [P]ext (r = 0.82, P < 0.05) conditions. The novel scanner-based rhizobox system used addresses the substantial limitations associated with current use of rhizoboxes to study root growth dynamics. |
| first_indexed | 2025-11-14T19:55:16Z |
| format | Article |
| id | nottingham-44350 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:55:16Z |
| publishDate | 2017 |
| publisher | Japanese Society for Root Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-443502020-05-04T18:45:41Z https://eprints.nottingham.ac.uk/44350/ A scanner-based rhizobox system enabling the quantification of root system development and response of Brassica rapa seedlings to external P availability Adu, Michael Osei Yawson, David O. Bennett, Malcolm J. Broadley, Martin R. Dupuy, Lionel X. White, Philip J. Rhizoboxes are soil-root compartments that may well provide the closest naturalistic conditions for studying root systems architectures (RSAs) in controlled environments. Rhizobox-based studies can however lead to mis-estimation of root traits due to poor recovery of roots and loss of fine root features during washing of roots. We used a novel scanner-based rhizobox system to evaluate: (i) RSA traits of Brassica rapa genotypes; (ii) the relationship between root traits recorded from rhizoboxes and those of harvested roots and (iii) genotypic variation of seedlings in response to external P ([P] ext) availability. Brassica rapa genotypes were grown in soil-filled rhizoboxes abutting flatbed scanners and were watered once with either deionised water or a solution of 600 μM KH2PO4 to approximately 80% field capacity on a weight basis. Shoot and root P concentrations ([P]shoot and [P]root) of the B. rapa lines grown on different [P]ext were quantified. Visible root length at the surface of rhizoboxes constituted 85% of the total root length recovered from harvested root samples. High P supply induced a strong increase in [P]shoot in all genotypes (P < 0.001) whereas low P supply generally led to greater partitioning to roots. Seed P concentration and tissue P concentration were correlated only at low [P]ext. Total root length was strongly correlated with tissue P content under both low [P]ext (r = 0.81, P < 0.05) and high [P]ext (r = 0.82, P < 0.05) conditions. The novel scanner-based rhizobox system used addresses the substantial limitations associated with current use of rhizoboxes to study root growth dynamics. Japanese Society for Root Research 2017-05-16 Article PeerReviewed Adu, Michael Osei, Yawson, David O., Bennett, Malcolm J., Broadley, Martin R., Dupuy, Lionel X. and White, Philip J. (2017) A scanner-based rhizobox system enabling the quantification of root system development and response of Brassica rapa seedlings to external P availability. Plant Root, 11 . pp. 16-32. ISSN 1881-6754 Brassica rapa L. nutrient acquisition phosphorus rhizobox root system architecture scanner-based phenotyping https://www.jstage.jst.go.jp/article/plantroot/11/0/11_16/_article doi:10.3117/plantroot.11.16 doi:10.3117/plantroot.11.16 |
| spellingShingle | Brassica rapa L. nutrient acquisition phosphorus rhizobox root system architecture scanner-based phenotyping Adu, Michael Osei Yawson, David O. Bennett, Malcolm J. Broadley, Martin R. Dupuy, Lionel X. White, Philip J. A scanner-based rhizobox system enabling the quantification of root system development and response of Brassica rapa seedlings to external P availability |
| title | A scanner-based rhizobox system enabling the quantification
of root system development and response of Brassica rapa seedlings to external P availability |
| title_full | A scanner-based rhizobox system enabling the quantification
of root system development and response of Brassica rapa seedlings to external P availability |
| title_fullStr | A scanner-based rhizobox system enabling the quantification
of root system development and response of Brassica rapa seedlings to external P availability |
| title_full_unstemmed | A scanner-based rhizobox system enabling the quantification
of root system development and response of Brassica rapa seedlings to external P availability |
| title_short | A scanner-based rhizobox system enabling the quantification
of root system development and response of Brassica rapa seedlings to external P availability |
| title_sort | scanner-based rhizobox system enabling the quantification
of root system development and response of brassica rapa seedlings to external p availability |
| topic | Brassica rapa L. nutrient acquisition phosphorus rhizobox root system architecture scanner-based phenotyping |
| url | https://eprints.nottingham.ac.uk/44350/ https://eprints.nottingham.ac.uk/44350/ https://eprints.nottingham.ac.uk/44350/ |