Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia
Preeclampsia is a pregnancy-specific condition that leads to increased cardiovascular risk in later life. A decrease in cholesterol efflux capacity is linked to CVD. We hypothesized that in preeclampsia there would be a disruption of maternal/fetal plasma to efflux cholesterol, as well as difference...
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American Society for Biochemistry and Molecular Biology
2017
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| Online Access: | https://eprints.nottingham.ac.uk/44336/ |
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| author | Mistry, Hiten D. Kurlak, L.O. Mansour, Yosef Zurkinden, Line Mohaupt, Markus Escher, Genevieve |
| author_facet | Mistry, Hiten D. Kurlak, L.O. Mansour, Yosef Zurkinden, Line Mohaupt, Markus Escher, Genevieve |
| author_sort | Mistry, Hiten D. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Preeclampsia is a pregnancy-specific condition that leads to increased cardiovascular risk in later life. A decrease in cholesterol efflux capacity is linked to CVD. We hypothesized that in preeclampsia there would be a disruption of maternal/fetal plasma to efflux cholesterol, as well as differences in the concentrations of both placental sterol 27-hydroxylase (CYP27A1) and apoA1 binding protein (AIBP). Total, HDL-, and ABCA1-mediated cholesterol effluxes were performed with maternal and fetal plasma from women with preeclampsia and normotensive controls (both n = 17). apoA1 and apoE were quantified by chemiluminescence, and 27-hydroxycholesterol (27-OHC) by GC-MS. Immunohistochemistry was used to determine placental expression/localization of CYP27A1, AIBP, apoA1, apoE, and SRB1. Maternal and fetal total and HDL-mediated cholesterol efflux capacities were increased in preeclampsia (by 10–20%), but ABCA1-mediated efflux was decreased (by 20–35%; P < 0.05). Maternal and fetal apoE concentrations were higher in preeclampsia. Fetal plasma 27-OHC levels were decreased in preeclamptic samples (P < 0.05). Placental protein expression of both CYP27A1 and AIBP were localized around fetal vessels and significantly increased in preeclampsia (P = 0.04). Placental 27-OHC concentrations were also raised in preeclampsia (P < 0.05). Increased HDL-mediated cholesterol efflux capacity and placental CYP27A1/27-OHC could be a rescue mechanism in preeclampsia, to remove cholesterol from cells to limit lipid peroxidation and increase placental angiogenesis. |
| first_indexed | 2025-11-14T19:55:13Z |
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| id | nottingham-44336 |
| institution | University of Nottingham Malaysia Campus |
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| last_indexed | 2025-11-14T19:55:13Z |
| publishDate | 2017 |
| publisher | American Society for Biochemistry and Molecular Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-443362020-05-04T18:49:28Z https://eprints.nottingham.ac.uk/44336/ Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia Mistry, Hiten D. Kurlak, L.O. Mansour, Yosef Zurkinden, Line Mohaupt, Markus Escher, Genevieve Preeclampsia is a pregnancy-specific condition that leads to increased cardiovascular risk in later life. A decrease in cholesterol efflux capacity is linked to CVD. We hypothesized that in preeclampsia there would be a disruption of maternal/fetal plasma to efflux cholesterol, as well as differences in the concentrations of both placental sterol 27-hydroxylase (CYP27A1) and apoA1 binding protein (AIBP). Total, HDL-, and ABCA1-mediated cholesterol effluxes were performed with maternal and fetal plasma from women with preeclampsia and normotensive controls (both n = 17). apoA1 and apoE were quantified by chemiluminescence, and 27-hydroxycholesterol (27-OHC) by GC-MS. Immunohistochemistry was used to determine placental expression/localization of CYP27A1, AIBP, apoA1, apoE, and SRB1. Maternal and fetal total and HDL-mediated cholesterol efflux capacities were increased in preeclampsia (by 10–20%), but ABCA1-mediated efflux was decreased (by 20–35%; P < 0.05). Maternal and fetal apoE concentrations were higher in preeclampsia. Fetal plasma 27-OHC levels were decreased in preeclamptic samples (P < 0.05). Placental protein expression of both CYP27A1 and AIBP were localized around fetal vessels and significantly increased in preeclampsia (P = 0.04). Placental 27-OHC concentrations were also raised in preeclampsia (P < 0.05). Increased HDL-mediated cholesterol efflux capacity and placental CYP27A1/27-OHC could be a rescue mechanism in preeclampsia, to remove cholesterol from cells to limit lipid peroxidation and increase placental angiogenesis. American Society for Biochemistry and Molecular Biology 2017-06-10 Article PeerReviewed Mistry, Hiten D., Kurlak, L.O., Mansour, Yosef, Zurkinden, Line, Mohaupt, Markus and Escher, Genevieve (2017) Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia. Journal of Lipid Research, 58 . pp. 1186-1185. ISSN 1539-7262 lipid/efflux; 27-hydroxycholesterol; apolipoproteins; pregnancy; hypertension; sterol 27-hydroxylase http://www.jlr.org/content/58/6/1186.short doi:10.1194/jlr.M071985 doi:10.1194/jlr.M071985 |
| spellingShingle | lipid/efflux; 27-hydroxycholesterol; apolipoproteins; pregnancy; hypertension; sterol 27-hydroxylase Mistry, Hiten D. Kurlak, L.O. Mansour, Yosef Zurkinden, Line Mohaupt, Markus Escher, Genevieve Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia |
| title | Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia |
| title_full | Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia |
| title_fullStr | Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia |
| title_full_unstemmed | Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia |
| title_short | Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia |
| title_sort | increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia |
| topic | lipid/efflux; 27-hydroxycholesterol; apolipoproteins; pregnancy; hypertension; sterol 27-hydroxylase |
| url | https://eprints.nottingham.ac.uk/44336/ https://eprints.nottingham.ac.uk/44336/ https://eprints.nottingham.ac.uk/44336/ |