Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation
Aldosterone is an important factor supporting placental growth and fetal development. Recently, expression of placental growth factor (PlGF) has been observed in response to aldosterone exposure in different models of atherosclerosis. Thus, we hypothesized that aldosterone up-regulates growth-adapti...
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| Format: | Article |
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Elsevier
2016
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| Online Access: | https://eprints.nottingham.ac.uk/44334/ |
| _version_ | 1848796893131309056 |
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| author | Eisele, Nicole Albrecht, Christiane Mistry, Hiten D. Dick, Bernhard Baumann, Marc Surbeck, Daniel Currie, Gemma Delles, Christian Mohaupt, Markus Escher, Genevieve Gennari-Moser, Carine |
| author_facet | Eisele, Nicole Albrecht, Christiane Mistry, Hiten D. Dick, Bernhard Baumann, Marc Surbeck, Daniel Currie, Gemma Delles, Christian Mohaupt, Markus Escher, Genevieve Gennari-Moser, Carine |
| author_sort | Eisele, Nicole |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Aldosterone is an important factor supporting placental growth and fetal development. Recently, expression of placental growth factor (PlGF) has been observed in response to aldosterone exposure in different models of atherosclerosis. Thus, we hypothesized that aldosterone up-regulates growth-adaptive angiogenesis in pregnancy, via increased placental PlGF expression.
We followed normotensive pregnant women (n = 24) throughout pregnancy and confirmed these results in a second independent first trimester cohort (n = 36). Urinary tetrahydroaldosterone was measured by gas chromatography-mass spectrometry and corrected for creatinine. Circulating PlGF concentrations were determined by ELISA. Additionally, cultured cell lines, adrenocortical H295R and choriocarcinoma BeWo cells, as well as primary human third trimester trophoblasts were tested in vitro. PlGF serum concentrations positively correlated with urinary tetrahydroaldosterone corrected for creatinine in these two independent cohorts. This observation was not due to PlGF, which did not induce aldosterone production in cultured H295R cells. On the other hand, PlGF expression was specifically enhanced by aldosterone in the presence of forskolin (p < 0.01) in trophoblasts. A pronounced stimulation of PlGF expression was observed with reduced glucose concentrations simulating starvation (p < 0.001).
In conclusion, aldosterone stimulates placental PlGF production, enhancing its availability during human pregnancy, a response amplified by reduced glucose supply. Given the crucial role of PlGF in maintaining a healthy pregnancy, these data support a key role of aldosterone for a healthy pregnancy outcome. |
| first_indexed | 2025-11-14T19:55:13Z |
| format | Article |
| id | nottingham-44334 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:55:13Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-443342020-05-04T17:38:42Z https://eprints.nottingham.ac.uk/44334/ Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation Eisele, Nicole Albrecht, Christiane Mistry, Hiten D. Dick, Bernhard Baumann, Marc Surbeck, Daniel Currie, Gemma Delles, Christian Mohaupt, Markus Escher, Genevieve Gennari-Moser, Carine Aldosterone is an important factor supporting placental growth and fetal development. Recently, expression of placental growth factor (PlGF) has been observed in response to aldosterone exposure in different models of atherosclerosis. Thus, we hypothesized that aldosterone up-regulates growth-adaptive angiogenesis in pregnancy, via increased placental PlGF expression. We followed normotensive pregnant women (n = 24) throughout pregnancy and confirmed these results in a second independent first trimester cohort (n = 36). Urinary tetrahydroaldosterone was measured by gas chromatography-mass spectrometry and corrected for creatinine. Circulating PlGF concentrations were determined by ELISA. Additionally, cultured cell lines, adrenocortical H295R and choriocarcinoma BeWo cells, as well as primary human third trimester trophoblasts were tested in vitro. PlGF serum concentrations positively correlated with urinary tetrahydroaldosterone corrected for creatinine in these two independent cohorts. This observation was not due to PlGF, which did not induce aldosterone production in cultured H295R cells. On the other hand, PlGF expression was specifically enhanced by aldosterone in the presence of forskolin (p < 0.01) in trophoblasts. A pronounced stimulation of PlGF expression was observed with reduced glucose concentrations simulating starvation (p < 0.001). In conclusion, aldosterone stimulates placental PlGF production, enhancing its availability during human pregnancy, a response amplified by reduced glucose supply. Given the crucial role of PlGF in maintaining a healthy pregnancy, these data support a key role of aldosterone for a healthy pregnancy outcome. Elsevier 2016-04-01 Article PeerReviewed Eisele, Nicole, Albrecht, Christiane, Mistry, Hiten D., Dick, Bernhard, Baumann, Marc, Surbeck, Daniel, Currie, Gemma, Delles, Christian, Mohaupt, Markus, Escher, Genevieve and Gennari-Moser, Carine (2016) Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation. Placenta, 40 . pp. 18-24. ISSN 1532-3102 Aldosterone; Placental growth factor; Pregnancy; Glucose; Trophoblast http://www.sciencedirect.com/science/article/pii/S0143400416300248 doi:10.1016/j.placenta.2016.02.004 doi:10.1016/j.placenta.2016.02.004 |
| spellingShingle | Aldosterone; Placental growth factor; Pregnancy; Glucose; Trophoblast Eisele, Nicole Albrecht, Christiane Mistry, Hiten D. Dick, Bernhard Baumann, Marc Surbeck, Daniel Currie, Gemma Delles, Christian Mohaupt, Markus Escher, Genevieve Gennari-Moser, Carine Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation |
| title | Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation |
| title_full | Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation |
| title_fullStr | Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation |
| title_full_unstemmed | Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation |
| title_short | Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation |
| title_sort | placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation |
| topic | Aldosterone; Placental growth factor; Pregnancy; Glucose; Trophoblast |
| url | https://eprints.nottingham.ac.uk/44334/ https://eprints.nottingham.ac.uk/44334/ https://eprints.nottingham.ac.uk/44334/ |