Gestation-specific reference intervals for comprehensive spot urinary steroid hormone metabolite analysis in normal singleton pregnancy and 6 weeks postpartum

Background: Normal pregnancy depends on pronounced adaptations in steroid hormone concentrations. Although in recent years, the understanding of these hormones in pregnancy has improved, the interpretation is hampered by insufficient reference values. Our aim was to establish gestation-specific refe...

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Main Authors: Mistry, Hiten D., Eisele, Nicole, Escher, Genevieve, Dick, Bernhard, Surbeck, Daniel, Delles, Christian, Currie, Gemma, Schlembach, Dietmar, Mohaupt, Markus, Gennari-Moser, Carine
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Published: BioMed Central 2015
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Online Access:https://eprints.nottingham.ac.uk/44330/
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author Mistry, Hiten D.
Eisele, Nicole
Escher, Genevieve
Dick, Bernhard
Surbeck, Daniel
Delles, Christian
Currie, Gemma
Schlembach, Dietmar
Mohaupt, Markus
Gennari-Moser, Carine
author_facet Mistry, Hiten D.
Eisele, Nicole
Escher, Genevieve
Dick, Bernhard
Surbeck, Daniel
Delles, Christian
Currie, Gemma
Schlembach, Dietmar
Mohaupt, Markus
Gennari-Moser, Carine
author_sort Mistry, Hiten D.
building Nottingham Research Data Repository
collection Online Access
description Background: Normal pregnancy depends on pronounced adaptations in steroid hormone concentrations. Although in recent years, the understanding of these hormones in pregnancy has improved, the interpretation is hampered by insufficient reference values. Our aim was to establish gestation-specific reference intervals for spot urinary steroid hormone levels in normal singleton pregnancies and 6 weeks postpartum. Methods: Cross-sectional multicentre observational study. Women recruited between 2008 and 2013 at 3 University Hospitals in Switzerland (Bern), Scotland (Glasgow) and Austria (Graz). Spot urine was collected from healthy women undergoing a normal pregnancy (age, 16-45 years; mean, 31 years) attending routine antenatal clinics at gestation weeks 11, 20, and 28 and approximately 6 weeks postpartum. Urine steroid hormone levels were analysed using gas-chromatography mass spectrometry. Creatinine was also measured by routine analysis and used for normalisation. Results: From the results, a reference interval was calculated for each hormone metabolite at each trimester and 6 weeks postpartum. Changes in these concentrations between trimesters and postpartum were also observed for several steroid hormones and followed changes proposed for index steroid hormones. Conclusions: Normal gestation-specific reference values for spot urinary steroid hormones throughout pregnancy and early postpartum are now available to facilitate clinical management and research approaches to steroid hormone metabolism in pregnancy and the early postpartum period.
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spelling nottingham-443302020-05-04T17:17:47Z https://eprints.nottingham.ac.uk/44330/ Gestation-specific reference intervals for comprehensive spot urinary steroid hormone metabolite analysis in normal singleton pregnancy and 6 weeks postpartum Mistry, Hiten D. Eisele, Nicole Escher, Genevieve Dick, Bernhard Surbeck, Daniel Delles, Christian Currie, Gemma Schlembach, Dietmar Mohaupt, Markus Gennari-Moser, Carine Background: Normal pregnancy depends on pronounced adaptations in steroid hormone concentrations. Although in recent years, the understanding of these hormones in pregnancy has improved, the interpretation is hampered by insufficient reference values. Our aim was to establish gestation-specific reference intervals for spot urinary steroid hormone levels in normal singleton pregnancies and 6 weeks postpartum. Methods: Cross-sectional multicentre observational study. Women recruited between 2008 and 2013 at 3 University Hospitals in Switzerland (Bern), Scotland (Glasgow) and Austria (Graz). Spot urine was collected from healthy women undergoing a normal pregnancy (age, 16-45 years; mean, 31 years) attending routine antenatal clinics at gestation weeks 11, 20, and 28 and approximately 6 weeks postpartum. Urine steroid hormone levels were analysed using gas-chromatography mass spectrometry. Creatinine was also measured by routine analysis and used for normalisation. Results: From the results, a reference interval was calculated for each hormone metabolite at each trimester and 6 weeks postpartum. Changes in these concentrations between trimesters and postpartum were also observed for several steroid hormones and followed changes proposed for index steroid hormones. Conclusions: Normal gestation-specific reference values for spot urinary steroid hormones throughout pregnancy and early postpartum are now available to facilitate clinical management and research approaches to steroid hormone metabolism in pregnancy and the early postpartum period. BioMed Central 2015-09-04 Article PeerReviewed Mistry, Hiten D., Eisele, Nicole, Escher, Genevieve, Dick, Bernhard, Surbeck, Daniel, Delles, Christian, Currie, Gemma, Schlembach, Dietmar, Mohaupt, Markus and Gennari-Moser, Carine (2015) Gestation-specific reference intervals for comprehensive spot urinary steroid hormone metabolite analysis in normal singleton pregnancy and 6 weeks postpartum. Reproductive Biology and Endocrinology, 13 . 101/1-101/13. ISSN 1477-7827 Pregnancy reference values; Urine; Steroid metabolites https://rbej.biomedcentral.com/articles/10.1186/s12958-015-0100-6
spellingShingle Pregnancy reference values; Urine; Steroid metabolites
Mistry, Hiten D.
Eisele, Nicole
Escher, Genevieve
Dick, Bernhard
Surbeck, Daniel
Delles, Christian
Currie, Gemma
Schlembach, Dietmar
Mohaupt, Markus
Gennari-Moser, Carine
Gestation-specific reference intervals for comprehensive spot urinary steroid hormone metabolite analysis in normal singleton pregnancy and 6 weeks postpartum
title Gestation-specific reference intervals for comprehensive spot urinary steroid hormone metabolite analysis in normal singleton pregnancy and 6 weeks postpartum
title_full Gestation-specific reference intervals for comprehensive spot urinary steroid hormone metabolite analysis in normal singleton pregnancy and 6 weeks postpartum
title_fullStr Gestation-specific reference intervals for comprehensive spot urinary steroid hormone metabolite analysis in normal singleton pregnancy and 6 weeks postpartum
title_full_unstemmed Gestation-specific reference intervals for comprehensive spot urinary steroid hormone metabolite analysis in normal singleton pregnancy and 6 weeks postpartum
title_short Gestation-specific reference intervals for comprehensive spot urinary steroid hormone metabolite analysis in normal singleton pregnancy and 6 weeks postpartum
title_sort gestation-specific reference intervals for comprehensive spot urinary steroid hormone metabolite analysis in normal singleton pregnancy and 6 weeks postpartum
topic Pregnancy reference values; Urine; Steroid metabolites
url https://eprints.nottingham.ac.uk/44330/
https://eprints.nottingham.ac.uk/44330/