Urine protein concentration estimation for biomarker discovery
Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-aci...
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| Format: | Article |
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Elsevier
2013
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| Online Access: | https://eprints.nottingham.ac.uk/44323/ |
| _version_ | 1848796889964609536 |
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| author | Mistry, Hiten D. Bramham, Kate Weston, Andrew Ward, Malcolm Thompson, Andrew Chappell, Lucy C. |
| author_facet | Mistry, Hiten D. Bramham, Kate Weston, Andrew Ward, Malcolm Thompson, Andrew Chappell, Lucy C. |
| author_sort | Mistry, Hiten D. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia. BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation. |
| first_indexed | 2025-11-14T19:55:10Z |
| format | Article |
| id | nottingham-44323 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:55:10Z |
| publishDate | 2013 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-443232020-05-04T20:18:44Z https://eprints.nottingham.ac.uk/44323/ Urine protein concentration estimation for biomarker discovery Mistry, Hiten D. Bramham, Kate Weston, Andrew Ward, Malcolm Thompson, Andrew Chappell, Lucy C. Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia. BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation. Elsevier 2013-10 Article PeerReviewed Mistry, Hiten D., Bramham, Kate, Weston, Andrew, Ward, Malcolm, Thompson, Andrew and Chappell, Lucy C. (2013) Urine protein concentration estimation for biomarker discovery. Pregnancy Hypertension, 3 (4). pp. 211-214. ISSN 2210-7797 Protein concentration assays; Proteomics; Urine http://www.sciencedirect.com/science/article/pii/S2210778913001980 doi:10.1016/j.preghy.2013.07.001 doi:10.1016/j.preghy.2013.07.001 |
| spellingShingle | Protein concentration assays; Proteomics; Urine Mistry, Hiten D. Bramham, Kate Weston, Andrew Ward, Malcolm Thompson, Andrew Chappell, Lucy C. Urine protein concentration estimation for biomarker discovery |
| title | Urine protein concentration estimation for biomarker discovery |
| title_full | Urine protein concentration estimation for biomarker discovery |
| title_fullStr | Urine protein concentration estimation for biomarker discovery |
| title_full_unstemmed | Urine protein concentration estimation for biomarker discovery |
| title_short | Urine protein concentration estimation for biomarker discovery |
| title_sort | urine protein concentration estimation for biomarker discovery |
| topic | Protein concentration assays; Proteomics; Urine |
| url | https://eprints.nottingham.ac.uk/44323/ https://eprints.nottingham.ac.uk/44323/ https://eprints.nottingham.ac.uk/44323/ |