Is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype

Artherosis of spiral arteries in uteroplacental beds from pre-eclamptic women resemble those of atherosclerosis, characterised by increased plasma lipids and lipoproteins. We hypothesised 1) lipoproteins receptors/transporter in placenta would be up-regulated in pre-eclampsia, associated with increa...

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Main Authors: Hentchke, Marta, Poli-de-Figueiredo, Carlos, Pinheiro da Costa, Bartira, Kurlak, L.O., Williams, Paula Juliet, Mistry, Hiten D.
Format: Article
Published: American Society for Biochemistry and Molecular Biology 2013
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Online Access:https://eprints.nottingham.ac.uk/44322/
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author Hentchke, Marta
Poli-de-Figueiredo, Carlos
Pinheiro da Costa, Bartira
Kurlak, L.O.
Williams, Paula Juliet
Mistry, Hiten D.
author_facet Hentchke, Marta
Poli-de-Figueiredo, Carlos
Pinheiro da Costa, Bartira
Kurlak, L.O.
Williams, Paula Juliet
Mistry, Hiten D.
author_sort Hentchke, Marta
building Nottingham Research Data Repository
collection Online Access
description Artherosis of spiral arteries in uteroplacental beds from pre-eclamptic women resemble those of atherosclerosis, characterised by increased plasma lipids and lipoproteins. We hypothesised 1) lipoproteins receptors/transporter in placenta would be up-regulated in pre-eclampsia, associated with increased maternal and fetal lipoprotein concentrations; 2) expression of these would be reduced in pre-eclamptic placentae from women delivering small-for-gestational-age (SGA) infants. Placental biopsies, maternal and umbilical serum samples were taken from 27 normotensive and 24 pre-eclamptic women. Maternal/umbilical cord serum LDL; HDL; total cholesterol and triglycerides were measured. Placental mRNA expression of lipoprotein receptors/transporters were quantified using qRT-PCR. Protein localisation/expression of LRP-1 in the pre-eclamptic with/without SGA was measured by immunohistochemistry. Placental mRNA expression of all genes except PON-1, MTTP and PDIA2 were observed. No differences for any lipoprotein receptors/transporters were found between groups; however, in the pre-eclamptic group placental LRP-1 expression was lower in SGA delivering mothers (n = 7; P=0.036). LRP-1 protein was localised around fetal vessels and Hofbauer cells. This is the first detailed study of maternal/fetal lipoprotein concentrations and placental lipoprotein receptor mRNA expression in normotensive and pre-eclamptic pregnancies. These findings do not support a role of altered lipid metabolism in pre-eclampsia, but may be involved in fetal growth.
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spelling nottingham-443222020-05-04T16:39:08Z https://eprints.nottingham.ac.uk/44322/ Is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype Hentchke, Marta Poli-de-Figueiredo, Carlos Pinheiro da Costa, Bartira Kurlak, L.O. Williams, Paula Juliet Mistry, Hiten D. Artherosis of spiral arteries in uteroplacental beds from pre-eclamptic women resemble those of atherosclerosis, characterised by increased plasma lipids and lipoproteins. We hypothesised 1) lipoproteins receptors/transporter in placenta would be up-regulated in pre-eclampsia, associated with increased maternal and fetal lipoprotein concentrations; 2) expression of these would be reduced in pre-eclamptic placentae from women delivering small-for-gestational-age (SGA) infants. Placental biopsies, maternal and umbilical serum samples were taken from 27 normotensive and 24 pre-eclamptic women. Maternal/umbilical cord serum LDL; HDL; total cholesterol and triglycerides were measured. Placental mRNA expression of lipoprotein receptors/transporters were quantified using qRT-PCR. Protein localisation/expression of LRP-1 in the pre-eclamptic with/without SGA was measured by immunohistochemistry. Placental mRNA expression of all genes except PON-1, MTTP and PDIA2 were observed. No differences for any lipoprotein receptors/transporters were found between groups; however, in the pre-eclamptic group placental LRP-1 expression was lower in SGA delivering mothers (n = 7; P=0.036). LRP-1 protein was localised around fetal vessels and Hofbauer cells. This is the first detailed study of maternal/fetal lipoprotein concentrations and placental lipoprotein receptor mRNA expression in normotensive and pre-eclamptic pregnancies. These findings do not support a role of altered lipid metabolism in pre-eclampsia, but may be involved in fetal growth. American Society for Biochemistry and Molecular Biology 2013-10-31 Article PeerReviewed Hentchke, Marta, Poli-de-Figueiredo, Carlos, Pinheiro da Costa, Bartira, Kurlak, L.O., Williams, Paula Juliet and Mistry, Hiten D. (2013) Is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype. Journal of Lipid Research, 54 . pp. 2658-2664. ISSN 1539-7262 Hypertension; Lipids; LDL cholesterol; HDL cholesterol; Triglycerides; ABCA1; LRP-1; LDL-R; SRB-1;Fetal growth http://www.jlr.org/content/54/10/2658 doi:10.1194/jlr.M036699 doi:10.1194/jlr.M036699
spellingShingle Hypertension; Lipids; LDL cholesterol; HDL cholesterol; Triglycerides; ABCA1; LRP-1; LDL-R; SRB-1;Fetal growth
Hentchke, Marta
Poli-de-Figueiredo, Carlos
Pinheiro da Costa, Bartira
Kurlak, L.O.
Williams, Paula Juliet
Mistry, Hiten D.
Is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype
title Is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype
title_full Is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype
title_fullStr Is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype
title_full_unstemmed Is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype
title_short Is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype
title_sort is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype
topic Hypertension; Lipids; LDL cholesterol; HDL cholesterol; Triglycerides; ABCA1; LRP-1; LDL-R; SRB-1;Fetal growth
url https://eprints.nottingham.ac.uk/44322/
https://eprints.nottingham.ac.uk/44322/
https://eprints.nottingham.ac.uk/44322/