Thyroid hormones and their placental deiodination in normal and pre-eclamptic pregnancy
Pre-eclampsia is associated with lower serum selenium concentrations and glutathione peroxidase expression/activity; total thyroid hormones are also lower. Objectives, study design and main outcome measures: We hypothesised that the placental selenoprotein deiodinase (D3) will be protected in pre-e...
| Main Authors: | , , , , |
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| Format: | Article |
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Elsevier
2013
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| Online Access: | https://eprints.nottingham.ac.uk/44274/ |
| _version_ | 1848796878105214976 |
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| author | Kurlak, L.O. Mistry, H.D. Kaptein, E. Visser, T.J. Broughton Pipkin, Fiona |
| author_facet | Kurlak, L.O. Mistry, H.D. Kaptein, E. Visser, T.J. Broughton Pipkin, Fiona |
| author_sort | Kurlak, L.O. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Pre-eclampsia is associated with lower serum selenium concentrations and glutathione peroxidase expression/activity; total thyroid hormones are also lower.
Objectives, study design and main outcome measures: We hypothesised that the placental selenoprotein deiodinase (D3) will be protected in pre-eclampsia due to the hierarchy of selenoprotein biosynthesis in selenium deficiency. Venous blood and tissue from three standardised placental sites were obtained at delivery from 27 normotensive and 23 pre-eclamptic women. mRNA expression and enzyme activity were assessed for both deiodinases (D2 and D3); protein expression/localisation was also measured for D3. FT4, FT3 and TSH concentrations were measured in maternal and umbilical cord blood.
Results: No significant differences in D3 mRNA or protein expression between normotensive and pre-eclamptic pregnancies. There was a significant effect of sampling site on placental D3 activity only in pre-eclamptic women (P = 0.034; highest activity nearest the cord). A strong correlation between D3 mRNA expression and enzyme activity existed only in the pre-eclamptic group; further strengthened when controlling for maternal selenium (P < 0.002). No significant differences were observed between groups for any of the maternal thyroid hormones; umbilical TSH concentrations were significantly higher in the pre-eclamptic samples (P < 0.001).
Conclusions: D3 mRNA and protein expression appear to be independent of selenium status. Nevertheless, the positive correlation between D3 mRNA expression and activity evident only in pre-eclampsia, suggests that in normotensive controls, where selenium is higher, translation is not affected, but in pre-eclampsia, where selenium is low, enzyme regulation may be altered. The raised umbilical TSH concentrations in pre-eclampsia may be an adaptive fetal response to maximise iodide uptake. |
| first_indexed | 2025-11-14T19:54:58Z |
| format | Article |
| id | nottingham-44274 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:54:58Z |
| publishDate | 2013 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-442742020-05-04T16:36:09Z https://eprints.nottingham.ac.uk/44274/ Thyroid hormones and their placental deiodination in normal and pre-eclamptic pregnancy Kurlak, L.O. Mistry, H.D. Kaptein, E. Visser, T.J. Broughton Pipkin, Fiona Pre-eclampsia is associated with lower serum selenium concentrations and glutathione peroxidase expression/activity; total thyroid hormones are also lower. Objectives, study design and main outcome measures: We hypothesised that the placental selenoprotein deiodinase (D3) will be protected in pre-eclampsia due to the hierarchy of selenoprotein biosynthesis in selenium deficiency. Venous blood and tissue from three standardised placental sites were obtained at delivery from 27 normotensive and 23 pre-eclamptic women. mRNA expression and enzyme activity were assessed for both deiodinases (D2 and D3); protein expression/localisation was also measured for D3. FT4, FT3 and TSH concentrations were measured in maternal and umbilical cord blood. Results: No significant differences in D3 mRNA or protein expression between normotensive and pre-eclamptic pregnancies. There was a significant effect of sampling site on placental D3 activity only in pre-eclamptic women (P = 0.034; highest activity nearest the cord). A strong correlation between D3 mRNA expression and enzyme activity existed only in the pre-eclamptic group; further strengthened when controlling for maternal selenium (P < 0.002). No significant differences were observed between groups for any of the maternal thyroid hormones; umbilical TSH concentrations were significantly higher in the pre-eclamptic samples (P < 0.001). Conclusions: D3 mRNA and protein expression appear to be independent of selenium status. Nevertheless, the positive correlation between D3 mRNA expression and activity evident only in pre-eclampsia, suggests that in normotensive controls, where selenium is higher, translation is not affected, but in pre-eclampsia, where selenium is low, enzyme regulation may be altered. The raised umbilical TSH concentrations in pre-eclampsia may be an adaptive fetal response to maximise iodide uptake. Elsevier 2013-05-01 Article PeerReviewed Kurlak, L.O., Mistry, H.D., Kaptein, E., Visser, T.J. and Broughton Pipkin, Fiona (2013) Thyroid hormones and their placental deiodination in normal and pre-eclamptic pregnancy. Placenta, 34 (5). pp. 395-400. ISSN 1532-3102 http://www.sciencedirect.com/science/article/pii/S0143400413001045?via%3Dihub doi:10.1016/j.placenta.2013.02.009 doi:10.1016/j.placenta.2013.02.009 |
| spellingShingle | Kurlak, L.O. Mistry, H.D. Kaptein, E. Visser, T.J. Broughton Pipkin, Fiona Thyroid hormones and their placental deiodination in normal and pre-eclamptic pregnancy |
| title | Thyroid hormones and their placental deiodination in normal and pre-eclamptic pregnancy |
| title_full | Thyroid hormones and their placental deiodination in normal and pre-eclamptic pregnancy |
| title_fullStr | Thyroid hormones and their placental deiodination in normal and pre-eclamptic pregnancy |
| title_full_unstemmed | Thyroid hormones and their placental deiodination in normal and pre-eclamptic pregnancy |
| title_short | Thyroid hormones and their placental deiodination in normal and pre-eclamptic pregnancy |
| title_sort | thyroid hormones and their placental deiodination in normal and pre-eclamptic pregnancy |
| url | https://eprints.nottingham.ac.uk/44274/ https://eprints.nottingham.ac.uk/44274/ https://eprints.nottingham.ac.uk/44274/ |