The placental renin-angiotensin system and oxidative stress in pre-eclampsia

There is an inverse correlation between human birthweight and umbilical venous angiotensin II (AngII) concentrations. Oxidative stress and increased pro-renin receptor (PRR) both enhance the cleavage of angiotensin I from angiotensinogen (AGT). Pre-eclampsia, a hypertensive disorder of pregnancy, ma...

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Main Authors: Mistry, H.D., Kurlak, L.O., Broughton Pipkin, Fiona
Format: Article
Published: Elsevier 2013
Online Access:https://eprints.nottingham.ac.uk/44270/
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author Mistry, H.D.
Kurlak, L.O.
Broughton Pipkin, Fiona
author_facet Mistry, H.D.
Kurlak, L.O.
Broughton Pipkin, Fiona
author_sort Mistry, H.D.
building Nottingham Research Data Repository
collection Online Access
description There is an inverse correlation between human birthweight and umbilical venous angiotensin II (AngII) concentrations. Oxidative stress and increased pro-renin receptor (PRR) both enhance the cleavage of angiotensin I from angiotensinogen (AGT). Pre-eclampsia, a hypertensive disorder of pregnancy, manifests as high blood pressure and proteinuria, and is a state of increased oxidative stress. Objectives, study design and main outcome measures Hypothesis: Pre-eclampsia will be associated with increased placental expression of components of the renin–angiotensin system, which could result in reduced infant birthweight. Biopsies were taken 1 cm from the placental edge from 27 normotensive controls and 23 pre-eclamptic White European women. Immunohistochemistry was performed for AGT, PRR, glutathione peroxidase 3 (GPx3) and the AT1R and AT2R AngII receptors. Protein expression was semi-quantitatively assessed (H-score). Results: AT1R expression was significantly increased in pre-eclamptic placentae, and negatively correlated with birthweight (r = −0.529, P = 0.009). AT1R expression was also negatively correlated with GPx3 expression overall (r = −0.647; P = 0.005). AT2R expression positively correlated with AGT (r = 0.615, P = 0.002) in the pre-eclamptic placentae only. Conclusions: The raised AT1R expression in pre-eclampsia, together with inadequate antioxidant protection, possibly through lower GPx activity, might enhance the vasoconstrictor effect of locally-generated AngII, contributing to the restricted fetal growth characteristic of pre-eclampsia. Conversely, the AT2R:AGT association within the pre-eclamptic placenta may provide a compensatory mechanism.
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spelling nottingham-442702020-05-04T16:35:08Z https://eprints.nottingham.ac.uk/44270/ The placental renin-angiotensin system and oxidative stress in pre-eclampsia Mistry, H.D. Kurlak, L.O. Broughton Pipkin, Fiona There is an inverse correlation between human birthweight and umbilical venous angiotensin II (AngII) concentrations. Oxidative stress and increased pro-renin receptor (PRR) both enhance the cleavage of angiotensin I from angiotensinogen (AGT). Pre-eclampsia, a hypertensive disorder of pregnancy, manifests as high blood pressure and proteinuria, and is a state of increased oxidative stress. Objectives, study design and main outcome measures Hypothesis: Pre-eclampsia will be associated with increased placental expression of components of the renin–angiotensin system, which could result in reduced infant birthweight. Biopsies were taken 1 cm from the placental edge from 27 normotensive controls and 23 pre-eclamptic White European women. Immunohistochemistry was performed for AGT, PRR, glutathione peroxidase 3 (GPx3) and the AT1R and AT2R AngII receptors. Protein expression was semi-quantitatively assessed (H-score). Results: AT1R expression was significantly increased in pre-eclamptic placentae, and negatively correlated with birthweight (r = −0.529, P = 0.009). AT1R expression was also negatively correlated with GPx3 expression overall (r = −0.647; P = 0.005). AT2R expression positively correlated with AGT (r = 0.615, P = 0.002) in the pre-eclamptic placentae only. Conclusions: The raised AT1R expression in pre-eclampsia, together with inadequate antioxidant protection, possibly through lower GPx activity, might enhance the vasoconstrictor effect of locally-generated AngII, contributing to the restricted fetal growth characteristic of pre-eclampsia. Conversely, the AT2R:AGT association within the pre-eclamptic placenta may provide a compensatory mechanism. Elsevier 2013-02-01 Article PeerReviewed Mistry, H.D., Kurlak, L.O. and Broughton Pipkin, Fiona (2013) The placental renin-angiotensin system and oxidative stress in pre-eclampsia. Placenta, 34 (2). pp. 182-186. ISSN 1532-3102 http://www.sciencedirect.com/science/article/pii/S0143400412004626?via%3Dihub doi:10.1016/j.placenta.2012.11.027 doi:10.1016/j.placenta.2012.11.027
spellingShingle Mistry, H.D.
Kurlak, L.O.
Broughton Pipkin, Fiona
The placental renin-angiotensin system and oxidative stress in pre-eclampsia
title The placental renin-angiotensin system and oxidative stress in pre-eclampsia
title_full The placental renin-angiotensin system and oxidative stress in pre-eclampsia
title_fullStr The placental renin-angiotensin system and oxidative stress in pre-eclampsia
title_full_unstemmed The placental renin-angiotensin system and oxidative stress in pre-eclampsia
title_short The placental renin-angiotensin system and oxidative stress in pre-eclampsia
title_sort placental renin-angiotensin system and oxidative stress in pre-eclampsia
url https://eprints.nottingham.ac.uk/44270/
https://eprints.nottingham.ac.uk/44270/
https://eprints.nottingham.ac.uk/44270/