MRI assessment of the postprandial gastrointestinal motility and peptide response in healthy humans
Background: Feeding triggers inter-related gastrointestinal (GI) motor, peptide and appetite responses. These are rarely studied together due to methodological limitations. Recent MRI advances allow pan-intestinal, non-invasive assessment of motility in the undisturbed gut. This study aimed to deve...
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| Format: | Article |
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Wiley
2018
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| Online Access: | https://eprints.nottingham.ac.uk/44215/ |
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| author | Khalaf, Asseel Hoad, Caroline Menys, Alex Nowak, Adam Taylor, Stuart A. Paparo, Stephen Lingaya, Melanie Falcone, Yirga Singh, Gulzar Spiller, Robin C. Gowland, Penny A. Marciani, Luca Moran, Gordon W. |
| author_facet | Khalaf, Asseel Hoad, Caroline Menys, Alex Nowak, Adam Taylor, Stuart A. Paparo, Stephen Lingaya, Melanie Falcone, Yirga Singh, Gulzar Spiller, Robin C. Gowland, Penny A. Marciani, Luca Moran, Gordon W. |
| author_sort | Khalaf, Asseel |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Background: Feeding triggers inter-related gastrointestinal (GI) motor, peptide and appetite responses. These are rarely studied together due to methodological limitations. Recent MRI advances allow pan-intestinal, non-invasive assessment of motility in the undisturbed gut.
This study aimed to develop a methodology to assess pan-intestinal motility and transit in a single session using MRI and compare imaging findings to GI peptide responses to a test meal and symptoms in a healthy volunteer cohort.
Methods: Fifteen healthy volunteers (29.3±2.7years and BMI 20.1±1.2Kg/m2) underwent baseline and postprandial MRI scans, symptom questionnaires and blood sampling (for subsequent GI peptide analysis, Glucagon-like peptide-1 (GLP-1), Polypeptide YY (PYY), Cholecystokinin (CCK)) at intervals for 270min following a 400g soup meal (204kcal, Heinz, UK). Gastric volume, gall bladder volume, small bowel water content, small bowel motility and whole gut transit were measured from the MRI scans.
Key Results: (mean±SEM) Small bowel motility index increased from fasting 39±3 arbitrary units (a.u.) to a maximum of 87±7a.u. immediately after feeding. PYY increased from fasting 98±10pg/ml to 149±14pg/ml at 30min and GLP-1 from fasting 15±3µg/ml to 22±4µg/ml. CCK increased from fasting 0.40±0.06pmol/ml to 0.94±0.1pmol/ml. Gastric volumes declined with a T1/2 of 46±5min and the gallbladder contracted from a fasting volume of 19±2ml to 12±2ml. Small bowel water content increased from 39±2ml to 51±2ml postprandial. Fullness VAS score increased from 9±5mm to 41±6mm at 30min postprandial.
Conclusions and Inferences: The test meal challenge was effective in inducing a change in MRI motility end-points which will improve understanding of the pathophysiological postprandial GI response. |
| first_indexed | 2025-11-14T19:54:45Z |
| format | Article |
| id | nottingham-44215 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:54:45Z |
| publishDate | 2018 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-442152020-05-04T19:53:06Z https://eprints.nottingham.ac.uk/44215/ MRI assessment of the postprandial gastrointestinal motility and peptide response in healthy humans Khalaf, Asseel Hoad, Caroline Menys, Alex Nowak, Adam Taylor, Stuart A. Paparo, Stephen Lingaya, Melanie Falcone, Yirga Singh, Gulzar Spiller, Robin C. Gowland, Penny A. Marciani, Luca Moran, Gordon W. Background: Feeding triggers inter-related gastrointestinal (GI) motor, peptide and appetite responses. These are rarely studied together due to methodological limitations. Recent MRI advances allow pan-intestinal, non-invasive assessment of motility in the undisturbed gut. This study aimed to develop a methodology to assess pan-intestinal motility and transit in a single session using MRI and compare imaging findings to GI peptide responses to a test meal and symptoms in a healthy volunteer cohort. Methods: Fifteen healthy volunteers (29.3±2.7years and BMI 20.1±1.2Kg/m2) underwent baseline and postprandial MRI scans, symptom questionnaires and blood sampling (for subsequent GI peptide analysis, Glucagon-like peptide-1 (GLP-1), Polypeptide YY (PYY), Cholecystokinin (CCK)) at intervals for 270min following a 400g soup meal (204kcal, Heinz, UK). Gastric volume, gall bladder volume, small bowel water content, small bowel motility and whole gut transit were measured from the MRI scans. Key Results: (mean±SEM) Small bowel motility index increased from fasting 39±3 arbitrary units (a.u.) to a maximum of 87±7a.u. immediately after feeding. PYY increased from fasting 98±10pg/ml to 149±14pg/ml at 30min and GLP-1 from fasting 15±3µg/ml to 22±4µg/ml. CCK increased from fasting 0.40±0.06pmol/ml to 0.94±0.1pmol/ml. Gastric volumes declined with a T1/2 of 46±5min and the gallbladder contracted from a fasting volume of 19±2ml to 12±2ml. Small bowel water content increased from 39±2ml to 51±2ml postprandial. Fullness VAS score increased from 9±5mm to 41±6mm at 30min postprandial. Conclusions and Inferences: The test meal challenge was effective in inducing a change in MRI motility end-points which will improve understanding of the pathophysiological postprandial GI response. Wiley 2018-01 Article PeerReviewed Khalaf, Asseel, Hoad, Caroline, Menys, Alex, Nowak, Adam, Taylor, Stuart A., Paparo, Stephen, Lingaya, Melanie, Falcone, Yirga, Singh, Gulzar, Spiller, Robin C., Gowland, Penny A., Marciani, Luca and Moran, Gordon W. (2018) MRI assessment of the postprandial gastrointestinal motility and peptide response in healthy humans. Neurogastroenterology and Motility, 30 (1). e13182/1-e13182/9. ISSN 1365-2982 Gastrointestinal motility; Gut peptides; MRI; Satiety; Bowel http://onlinelibrary.wiley.com/doi/10.1111/nmo.13182/full doi:10.1111/nmo.13182 doi:10.1111/nmo.13182 |
| spellingShingle | Gastrointestinal motility; Gut peptides; MRI; Satiety; Bowel Khalaf, Asseel Hoad, Caroline Menys, Alex Nowak, Adam Taylor, Stuart A. Paparo, Stephen Lingaya, Melanie Falcone, Yirga Singh, Gulzar Spiller, Robin C. Gowland, Penny A. Marciani, Luca Moran, Gordon W. MRI assessment of the postprandial gastrointestinal motility and peptide response in healthy humans |
| title | MRI assessment of the postprandial gastrointestinal motility and peptide response in healthy humans |
| title_full | MRI assessment of the postprandial gastrointestinal motility and peptide response in healthy humans |
| title_fullStr | MRI assessment of the postprandial gastrointestinal motility and peptide response in healthy humans |
| title_full_unstemmed | MRI assessment of the postprandial gastrointestinal motility and peptide response in healthy humans |
| title_short | MRI assessment of the postprandial gastrointestinal motility and peptide response in healthy humans |
| title_sort | mri assessment of the postprandial gastrointestinal motility and peptide response in healthy humans |
| topic | Gastrointestinal motility; Gut peptides; MRI; Satiety; Bowel |
| url | https://eprints.nottingham.ac.uk/44215/ https://eprints.nottingham.ac.uk/44215/ https://eprints.nottingham.ac.uk/44215/ |