The impact of detergents on the tissue decellularization process: a ToF-SIMS study
Biologic scaffolds are derived from mammalian tissues, which must be decellularized to remove cellular antigens that would otherwise incite an adverse immune response. Although widely used clinically, the optimum balance between cell removal and the disruption of matrix architecture and surface liga...
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| Format: | Article |
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Elsevier
2017
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| Online Access: | https://eprints.nottingham.ac.uk/44203/ |
| _version_ | 1848796861776789504 |
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| author | White, Lisa J. Taylor, Adam J. Faulk, Denver M. Keane, Timothy J. Saldin, Lindsey T. Reing, Janet E. Swinehart, Ilea T. Turner, Neill J. Ratner, Buddy D. Badylak, Stephen F. |
| author_facet | White, Lisa J. Taylor, Adam J. Faulk, Denver M. Keane, Timothy J. Saldin, Lindsey T. Reing, Janet E. Swinehart, Ilea T. Turner, Neill J. Ratner, Buddy D. Badylak, Stephen F. |
| author_sort | White, Lisa J. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Biologic scaffolds are derived from mammalian tissues, which must be decellularized to remove cellular antigens that would otherwise incite an adverse immune response. Although widely used clinically, the optimum balance between cell removal and the disruption of matrix architecture and surface ligand landscape remains a considerable challenge. Here we describe the use of time of flight secondary ion mass spectroscopy (ToF-SIMS) to provide sensitive, molecular specific, localized analysis of detergent decellularized biologic scaffolds. We detected residual detergent fragments, specifically from Triton X-100, sodium deoxycholate and sodium dodecyl sulphate (SDS) in decellularized scaffolds; increased SDS concentrations from 0.1% to 1.0% increased both the intensity of SDS fragments and adverse cell outcomes. We also identified cellular remnants, by detecting phosphate and phosphocholine ions in PAA and CHAPS decellularized scaffolds. The present study demonstrates ToF-SIMS is not only a powerful tool for characterization of biologic scaffold surface molecular functionality, but also enables sensitive assessment of decellularization efficacy. |
| first_indexed | 2025-11-14T19:54:43Z |
| format | Article |
| id | nottingham-44203 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:54:43Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-442032020-05-04T18:32:55Z https://eprints.nottingham.ac.uk/44203/ The impact of detergents on the tissue decellularization process: a ToF-SIMS study White, Lisa J. Taylor, Adam J. Faulk, Denver M. Keane, Timothy J. Saldin, Lindsey T. Reing, Janet E. Swinehart, Ilea T. Turner, Neill J. Ratner, Buddy D. Badylak, Stephen F. Biologic scaffolds are derived from mammalian tissues, which must be decellularized to remove cellular antigens that would otherwise incite an adverse immune response. Although widely used clinically, the optimum balance between cell removal and the disruption of matrix architecture and surface ligand landscape remains a considerable challenge. Here we describe the use of time of flight secondary ion mass spectroscopy (ToF-SIMS) to provide sensitive, molecular specific, localized analysis of detergent decellularized biologic scaffolds. We detected residual detergent fragments, specifically from Triton X-100, sodium deoxycholate and sodium dodecyl sulphate (SDS) in decellularized scaffolds; increased SDS concentrations from 0.1% to 1.0% increased both the intensity of SDS fragments and adverse cell outcomes. We also identified cellular remnants, by detecting phosphate and phosphocholine ions in PAA and CHAPS decellularized scaffolds. The present study demonstrates ToF-SIMS is not only a powerful tool for characterization of biologic scaffold surface molecular functionality, but also enables sensitive assessment of decellularization efficacy. Elsevier 2017-03-01 Article PeerReviewed White, Lisa J., Taylor, Adam J., Faulk, Denver M., Keane, Timothy J., Saldin, Lindsey T., Reing, Janet E., Swinehart, Ilea T., Turner, Neill J., Ratner, Buddy D. and Badylak, Stephen F. (2017) The impact of detergents on the tissue decellularization process: a ToF-SIMS study. Acta Biomaterialia, 50 . pp. 207-219. ISSN 1878-7568 extracellular matrix biologic scaffold decellularization ToF-SIMS detergents http://www.sciencedirect.com/science/article/pii/S1742706116307012?via%3Dihub doi.org/10.1016/j.actbio.2016.12.033 doi.org/10.1016/j.actbio.2016.12.033 |
| spellingShingle | extracellular matrix biologic scaffold decellularization ToF-SIMS detergents White, Lisa J. Taylor, Adam J. Faulk, Denver M. Keane, Timothy J. Saldin, Lindsey T. Reing, Janet E. Swinehart, Ilea T. Turner, Neill J. Ratner, Buddy D. Badylak, Stephen F. The impact of detergents on the tissue decellularization process: a ToF-SIMS study |
| title | The impact of detergents on the tissue decellularization process: a ToF-SIMS study |
| title_full | The impact of detergents on the tissue decellularization process: a ToF-SIMS study |
| title_fullStr | The impact of detergents on the tissue decellularization process: a ToF-SIMS study |
| title_full_unstemmed | The impact of detergents on the tissue decellularization process: a ToF-SIMS study |
| title_short | The impact of detergents on the tissue decellularization process: a ToF-SIMS study |
| title_sort | impact of detergents on the tissue decellularization process: a tof-sims study |
| topic | extracellular matrix biologic scaffold decellularization ToF-SIMS detergents |
| url | https://eprints.nottingham.ac.uk/44203/ https://eprints.nottingham.ac.uk/44203/ https://eprints.nottingham.ac.uk/44203/ |