Maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain
Enzyme saccharification of pretreated brewers spent grains (BSG) was investigated, aiming at maximising glucose production. Factors investigated were; variation of the solids loadings at different cellulolytic enzyme doses, reaction time, higher energy mixing methods, supplementation of the cellulol...
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| Format: | Article |
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Green Wave Publishing of Canada
2016
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| Online Access: | https://eprints.nottingham.ac.uk/44086/ |
| _version_ | 1848796834450898944 |
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| author | Wilkinson, Stuart Smart, Katherine A. James, Sue Cook, David J. |
| author_facet | Wilkinson, Stuart Smart, Katherine A. James, Sue Cook, David J. |
| author_sort | Wilkinson, Stuart |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Enzyme saccharification of pretreated brewers spent grains (BSG) was investigated, aiming at maximising glucose production. Factors investigated were; variation of the solids loadings at different cellulolytic enzyme doses, reaction time, higher energy mixing methods, supplementation of the cellulolytic enzymes with additional enzymes (and cofactors) and use of fed-batch methods. Improved slurry agitation through aerated high-torque mixing offered small but significant enhancements in glucose yields (to 53 ± 2.9 g/L and 45% of theoretical yield) compared to only 41 ± 4.0 g/L and 39% of theoretical yield for standard shaking methods (at 15% w/v solids loading). Supplementation of the cellulolytic enzymes with additional enzymes (acetyl xylan esterases, ferulic acid esterases and α-L- arabinofuranosidases) also boosted achieved glucose yields to 58 – 69 ± 0.8 - 6.2 g/L which equated to 52 - 58% of theoretical yield. Fed-batch methods also enhanced glucose yields (to 58 ± 2.2 g/L and 35% of theoretical yield at 25% w/v solids loading) compared to non-fed-batch methods. From these investigations a novel enzymatic saccharification method was developed (using enhanced mixing, a fed-batch approach and additional carbohydrate degrading enzymes) which further increased glucose yields to 78 ± 4.1 g/L and 43% of theoretical yield when operating at high solids loading (25% w/v). |
| first_indexed | 2025-11-14T19:54:17Z |
| format | Article |
| id | nottingham-44086 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:54:17Z |
| publishDate | 2016 |
| publisher | Green Wave Publishing of Canada |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-440862020-05-04T17:49:06Z https://eprints.nottingham.ac.uk/44086/ Maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain Wilkinson, Stuart Smart, Katherine A. James, Sue Cook, David J. Enzyme saccharification of pretreated brewers spent grains (BSG) was investigated, aiming at maximising glucose production. Factors investigated were; variation of the solids loadings at different cellulolytic enzyme doses, reaction time, higher energy mixing methods, supplementation of the cellulolytic enzymes with additional enzymes (and cofactors) and use of fed-batch methods. Improved slurry agitation through aerated high-torque mixing offered small but significant enhancements in glucose yields (to 53 ± 2.9 g/L and 45% of theoretical yield) compared to only 41 ± 4.0 g/L and 39% of theoretical yield for standard shaking methods (at 15% w/v solids loading). Supplementation of the cellulolytic enzymes with additional enzymes (acetyl xylan esterases, ferulic acid esterases and α-L- arabinofuranosidases) also boosted achieved glucose yields to 58 – 69 ± 0.8 - 6.2 g/L which equated to 52 - 58% of theoretical yield. Fed-batch methods also enhanced glucose yields (to 58 ± 2.2 g/L and 35% of theoretical yield at 25% w/v solids loading) compared to non-fed-batch methods. From these investigations a novel enzymatic saccharification method was developed (using enhanced mixing, a fed-batch approach and additional carbohydrate degrading enzymes) which further increased glucose yields to 78 ± 4.1 g/L and 43% of theoretical yield when operating at high solids loading (25% w/v). Green Wave Publishing of Canada 2016-06-01 Article PeerReviewed Wilkinson, Stuart, Smart, Katherine A., James, Sue and Cook, David J. (2016) Maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain. Biofuel Research Journal, 3 (2). pp. 417-429. ISSN 2292-8782 Brewers Spent Grains Bioethanol Enzymatic saccharification High solids loading https://www.biofueljournal.com/article_15101.html doi:10.18331/BRJ2016.3.2.7 doi:10.18331/BRJ2016.3.2.7 |
| spellingShingle | Brewers Spent Grains Bioethanol Enzymatic saccharification High solids loading Wilkinson, Stuart Smart, Katherine A. James, Sue Cook, David J. Maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain |
| title | Maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain |
| title_full | Maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain |
| title_fullStr | Maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain |
| title_full_unstemmed | Maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain |
| title_short | Maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain |
| title_sort | maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain |
| topic | Brewers Spent Grains Bioethanol Enzymatic saccharification High solids loading |
| url | https://eprints.nottingham.ac.uk/44086/ https://eprints.nottingham.ac.uk/44086/ https://eprints.nottingham.ac.uk/44086/ |