Acanthamoeba castellanii: a new high-throughput method for drug screening in vitro
Despite significant public health impact, there is no specific antiprotozoal therapy for prevention and treatment of Acanthamoeba castellanii infection. There is a need for new and efficient anti-Acanthamoeba drugs that are less toxic and can reduce treatment duration and frequency of administration...
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Elsevier
2016
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| Online Access: | https://eprints.nottingham.ac.uk/44052/ |
| _version_ | 1848796825813778432 |
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| author | Ortega-Rivas, Antonio Padrón, José M. Valladares, Basilio Elsheikha, Hany M. |
| author_facet | Ortega-Rivas, Antonio Padrón, José M. Valladares, Basilio Elsheikha, Hany M. |
| author_sort | Ortega-Rivas, Antonio |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Despite significant public health impact, there is no specific antiprotozoal therapy for prevention and treatment of Acanthamoeba castellanii infection. There is a need for new and efficient anti-Acanthamoeba drugs that are less toxic and can reduce treatment duration and frequency of administration. In this context a new, rapid and sensitive assay is required for high-throughput activity testing and screening of new therapeutic compounds. A colorimetric assay based on sulforhodamine B (SRB) staining has been developed for anti-Acanthamoeba drug susceptibility testing and adapted to a 96-well microtiter plate format. Under these conditions chlorhexidine was tested to validate the assay using two clinical strains of A. castellanii (Neff strain, T4 genotype [IC50 4.68±0.6 _M] and T3 genotype [IC50 5.69±0.9 _M]). These results were in good agreement with those obtained by the conventional Alamar Blue assay, OCR cytotoxicity assay and manual cell counting method. Our new assay offers an inexpensive and reliable method, which complements current assays by enhancing high-throughput anti-Acanthamoeba drug screening capabilities. |
| first_indexed | 2025-11-14T19:54:08Z |
| format | Article |
| id | nottingham-44052 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:54:08Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-440522020-05-04T18:18:56Z https://eprints.nottingham.ac.uk/44052/ Acanthamoeba castellanii: a new high-throughput method for drug screening in vitro Ortega-Rivas, Antonio Padrón, José M. Valladares, Basilio Elsheikha, Hany M. Despite significant public health impact, there is no specific antiprotozoal therapy for prevention and treatment of Acanthamoeba castellanii infection. There is a need for new and efficient anti-Acanthamoeba drugs that are less toxic and can reduce treatment duration and frequency of administration. In this context a new, rapid and sensitive assay is required for high-throughput activity testing and screening of new therapeutic compounds. A colorimetric assay based on sulforhodamine B (SRB) staining has been developed for anti-Acanthamoeba drug susceptibility testing and adapted to a 96-well microtiter plate format. Under these conditions chlorhexidine was tested to validate the assay using two clinical strains of A. castellanii (Neff strain, T4 genotype [IC50 4.68±0.6 _M] and T3 genotype [IC50 5.69±0.9 _M]). These results were in good agreement with those obtained by the conventional Alamar Blue assay, OCR cytotoxicity assay and manual cell counting method. Our new assay offers an inexpensive and reliable method, which complements current assays by enhancing high-throughput anti-Acanthamoeba drug screening capabilities. Elsevier 2016-12-01 Article PeerReviewed Ortega-Rivas, Antonio, Padrón, José M., Valladares, Basilio and Elsheikha, Hany M. (2016) Acanthamoeba castellanii: a new high-throughput method for drug screening in vitro. Acta Tropica, 164 . pp. 95-99. ISSN 0001-706X Acanthamoeba Colorimetric assay Drug screening Sulforhodamine B http://www.sciencedirect.com/science/article/pii/S0001706X16304533?via%3Dihub doi:10.1016/j.actatropica.2016.09.006 doi:10.1016/j.actatropica.2016.09.006 |
| spellingShingle | Acanthamoeba Colorimetric assay Drug screening Sulforhodamine B Ortega-Rivas, Antonio Padrón, José M. Valladares, Basilio Elsheikha, Hany M. Acanthamoeba castellanii: a new high-throughput method for drug screening in vitro |
| title | Acanthamoeba castellanii: a new high-throughput method for drug screening in vitro |
| title_full | Acanthamoeba castellanii: a new high-throughput method for drug screening in vitro |
| title_fullStr | Acanthamoeba castellanii: a new high-throughput method for drug screening in vitro |
| title_full_unstemmed | Acanthamoeba castellanii: a new high-throughput method for drug screening in vitro |
| title_short | Acanthamoeba castellanii: a new high-throughput method for drug screening in vitro |
| title_sort | acanthamoeba castellanii: a new high-throughput method for drug screening in vitro |
| topic | Acanthamoeba Colorimetric assay Drug screening Sulforhodamine B |
| url | https://eprints.nottingham.ac.uk/44052/ https://eprints.nottingham.ac.uk/44052/ https://eprints.nottingham.ac.uk/44052/ |