Anti-Food allergic activity of sulfated polysaccharide from Gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 MAPK

Polysaccharides from Gracilaria lemaneiformis in particular possess various bioactive functions, but their antiallergic activity remains incompletely defined. Sulfated polysaccharide from Gracilaria lemaneiformis (GLSP) was obtained by water extraction and ethanol precipitation followed by column ch...

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Main Authors: Liu, Qing-Mei, Yang, Yang, Maleki, Soheila J., Alcocer, Marcos, Xu, Sha-Sha, Shi, Chao-Lan, Cao, Min-Jie, Liu, Guang-Ming
Format: Article
Published: American Chemical Society 2016
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Online Access:https://eprints.nottingham.ac.uk/43933/
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author Liu, Qing-Mei
Yang, Yang
Maleki, Soheila J.
Alcocer, Marcos
Xu, Sha-Sha
Shi, Chao-Lan
Cao, Min-Jie
Liu, Guang-Ming
author_facet Liu, Qing-Mei
Yang, Yang
Maleki, Soheila J.
Alcocer, Marcos
Xu, Sha-Sha
Shi, Chao-Lan
Cao, Min-Jie
Liu, Guang-Ming
author_sort Liu, Qing-Mei
building Nottingham Research Data Repository
collection Online Access
description Polysaccharides from Gracilaria lemaneiformis in particular possess various bioactive functions, but their antiallergic activity remains incompletely defined. Sulfated polysaccharide from Gracilaria lemaneiformis (GLSP) was obtained by water extraction and ethanol precipitation followed by column chromatography. BALB/c mice, RBL-2H3, and KU812 cells were used for verifying the anti food allergic activity of GLSP. According to the results of mice experiment, GLSP was able to alleviate allergy symptoms, to reduce TM-specific IgE and IgG1, to suppress Th2 cell polarization, and to promote the function of regulatory T (Treg) cells. In addition, GLSP had the ability to inhibit the function of RBL-2H3 cells. Furthermore, GLSP inhibited the activation of KU812 via suppression of p38 mitogen-activated protein kinase (MAPK). In conclusion, immunosuppression as well as the reduction in the level of p38 MAPK may contribute to GLSP’s putative activity against food allergy. GLSP may be used as a functional food component for allergic patients.
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publishDate 2016
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spelling nottingham-439332020-05-04T17:51:37Z https://eprints.nottingham.ac.uk/43933/ Anti-Food allergic activity of sulfated polysaccharide from Gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 MAPK Liu, Qing-Mei Yang, Yang Maleki, Soheila J. Alcocer, Marcos Xu, Sha-Sha Shi, Chao-Lan Cao, Min-Jie Liu, Guang-Ming Polysaccharides from Gracilaria lemaneiformis in particular possess various bioactive functions, but their antiallergic activity remains incompletely defined. Sulfated polysaccharide from Gracilaria lemaneiformis (GLSP) was obtained by water extraction and ethanol precipitation followed by column chromatography. BALB/c mice, RBL-2H3, and KU812 cells were used for verifying the anti food allergic activity of GLSP. According to the results of mice experiment, GLSP was able to alleviate allergy symptoms, to reduce TM-specific IgE and IgG1, to suppress Th2 cell polarization, and to promote the function of regulatory T (Treg) cells. In addition, GLSP had the ability to inhibit the function of RBL-2H3 cells. Furthermore, GLSP inhibited the activation of KU812 via suppression of p38 mitogen-activated protein kinase (MAPK). In conclusion, immunosuppression as well as the reduction in the level of p38 MAPK may contribute to GLSP’s putative activity against food allergy. GLSP may be used as a functional food component for allergic patients. American Chemical Society 2016-05-17 Article PeerReviewed Liu, Qing-Mei, Yang, Yang, Maleki, Soheila J., Alcocer, Marcos, Xu, Sha-Sha, Shi, Chao-Lan, Cao, Min-Jie and Liu, Guang-Ming (2016) Anti-Food allergic activity of sulfated polysaccharide from Gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 MAPK. Journal of Agricultural and Food Chemistry, 64 (22). pp. 4536-4544. ISSN 0021-8561 food allergy; immunosuppression; p38 MAPK; sulfated polysaccharide from Gracilaria lemaneiformis http://pubs.acs.org/doi/abs/10.1021/acs.jafc.6b01086 doi:10.1021/acs.jafc.6b01086 doi:10.1021/acs.jafc.6b01086
spellingShingle food allergy; immunosuppression; p38 MAPK; sulfated polysaccharide from Gracilaria lemaneiformis
Liu, Qing-Mei
Yang, Yang
Maleki, Soheila J.
Alcocer, Marcos
Xu, Sha-Sha
Shi, Chao-Lan
Cao, Min-Jie
Liu, Guang-Ming
Anti-Food allergic activity of sulfated polysaccharide from Gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 MAPK
title Anti-Food allergic activity of sulfated polysaccharide from Gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 MAPK
title_full Anti-Food allergic activity of sulfated polysaccharide from Gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 MAPK
title_fullStr Anti-Food allergic activity of sulfated polysaccharide from Gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 MAPK
title_full_unstemmed Anti-Food allergic activity of sulfated polysaccharide from Gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 MAPK
title_short Anti-Food allergic activity of sulfated polysaccharide from Gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 MAPK
title_sort anti-food allergic activity of sulfated polysaccharide from gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 mapk
topic food allergy; immunosuppression; p38 MAPK; sulfated polysaccharide from Gracilaria lemaneiformis
url https://eprints.nottingham.ac.uk/43933/
https://eprints.nottingham.ac.uk/43933/
https://eprints.nottingham.ac.uk/43933/