Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus
A new Torradovirus tentatively named Carrot torrado virus (CaTV) was an incidental finding following a next generation sequencing study investigating internal vascular necrosis in carrot. The closest related viruses are Lettuce necrotic leaf curl virus (LNLCV) found in the Netherlands in 2011 and Mo...
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Elsevier
2016
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| Online Access: | https://eprints.nottingham.ac.uk/43907/ |
| _version_ | 1848796793936019456 |
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| author | Rozado-Aguirre, Zuriñe Adams, Ian Collins, Larissa Fox, Adrian Dickinson, Matthew Boonham, Neil |
| author_facet | Rozado-Aguirre, Zuriñe Adams, Ian Collins, Larissa Fox, Adrian Dickinson, Matthew Boonham, Neil |
| author_sort | Rozado-Aguirre, Zuriñe |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | A new Torradovirus tentatively named Carrot torrado virus (CaTV) was an incidental finding following a next generation sequencing study investigating internal vascular necrosis in carrot. The closest related viruses are Lettuce necrotic leaf curl virus (LNLCV) found in the Netherlands in 2011 and Motherwort yellow mottle virus (MYMoV) found in Korea in 2014. Primers for reverse transcriptase-PCR (RT-PCR) and RT-qPCR were designed with the aim of testing for the presence of virus in plant samples collected from the field. Both methods successfully amplified the target from infected samples but not from healthy control samples. The specificity of the CaTV assay was also checked against other known carrot viruses and no cross-reaction was seen. A comparative study between methods showed RT-qPCR was the most reliable method, giving positive results in samples where RT-PCR fails. Evaluation of the Ct values following RT-qPCR and a direct comparison demonstrated this was due to improved sensitivity. The previous published Torradovirus genus specific RT-PCR primers were tested and shown to detect CaTV. Also, virus transmission experiments carried out suggest that unlike other species of the same genus, Carrot torrado virus could be aphid-transmitted. |
| first_indexed | 2025-11-14T19:53:38Z |
| format | Article |
| id | nottingham-43907 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:53:38Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-439072020-05-04T18:04:45Z https://eprints.nottingham.ac.uk/43907/ Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus Rozado-Aguirre, Zuriñe Adams, Ian Collins, Larissa Fox, Adrian Dickinson, Matthew Boonham, Neil A new Torradovirus tentatively named Carrot torrado virus (CaTV) was an incidental finding following a next generation sequencing study investigating internal vascular necrosis in carrot. The closest related viruses are Lettuce necrotic leaf curl virus (LNLCV) found in the Netherlands in 2011 and Motherwort yellow mottle virus (MYMoV) found in Korea in 2014. Primers for reverse transcriptase-PCR (RT-PCR) and RT-qPCR were designed with the aim of testing for the presence of virus in plant samples collected from the field. Both methods successfully amplified the target from infected samples but not from healthy control samples. The specificity of the CaTV assay was also checked against other known carrot viruses and no cross-reaction was seen. A comparative study between methods showed RT-qPCR was the most reliable method, giving positive results in samples where RT-PCR fails. Evaluation of the Ct values following RT-qPCR and a direct comparison demonstrated this was due to improved sensitivity. The previous published Torradovirus genus specific RT-PCR primers were tested and shown to detect CaTV. Also, virus transmission experiments carried out suggest that unlike other species of the same genus, Carrot torrado virus could be aphid-transmitted. Elsevier 2016-09-01 Article PeerReviewed Rozado-Aguirre, Zuriñe, Adams, Ian, Collins, Larissa, Fox, Adrian, Dickinson, Matthew and Boonham, Neil (2016) Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus. Journal of Virological Methods, 235 . pp. 119-124. ISSN 0166-0934 Virus Carrots RT-PCR RT-qPCR http://www.sciencedirect.com/science/article/pii/S0166093415300987?via%3Dihub doi:10.1016/j.jviromet.2016.05.018 doi:10.1016/j.jviromet.2016.05.018 |
| spellingShingle | Virus Carrots RT-PCR RT-qPCR Rozado-Aguirre, Zuriñe Adams, Ian Collins, Larissa Fox, Adrian Dickinson, Matthew Boonham, Neil Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus |
| title | Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus |
| title_full | Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus |
| title_fullStr | Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus |
| title_full_unstemmed | Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus |
| title_short | Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus |
| title_sort | detection and transmission of carrot torrado virus, a novel putative member of the torradovirus genus |
| topic | Virus Carrots RT-PCR RT-qPCR |
| url | https://eprints.nottingham.ac.uk/43907/ https://eprints.nottingham.ac.uk/43907/ https://eprints.nottingham.ac.uk/43907/ |