Airway and parenchymal strains during bronchoconstriction in the precision cut lung slice
The precision-cut lung slice (PCLS) is a powerful tool for studying airway reactivity, but biomechanical measurements to date have largely focused on changes in airway caliber. Here we describe an image processing tool that reveals the associated spatio-temporal changes in airway and parenchymal str...
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| Format: | Article |
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Frontiers Media
2016
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| Online Access: | https://eprints.nottingham.ac.uk/43885/ |
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| author | Hiorns, Jonathan E. Bidan, Cécile M. Jensen, Oliver E. Gosens, Reinoud Kistemaker, Loes E.M. Fredberg, Jeffrey J. Butler, Jim P. Krishnan, Ramaswamy Brook, Bindi S. |
| author_facet | Hiorns, Jonathan E. Bidan, Cécile M. Jensen, Oliver E. Gosens, Reinoud Kistemaker, Loes E.M. Fredberg, Jeffrey J. Butler, Jim P. Krishnan, Ramaswamy Brook, Bindi S. |
| author_sort | Hiorns, Jonathan E. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The precision-cut lung slice (PCLS) is a powerful tool for studying airway reactivity, but biomechanical measurements to date have largely focused on changes in airway caliber. Here we describe an image processing tool that reveals the associated spatio-temporal changes in airway and parenchymal strains. Displacements of sub-regions within the PCLS are tracked in phase-contrast movies acquired after addition of contractile and relaxing drugs. From displacement maps, strains are determined across the entire PCLS or along user-specified directions. In a representative mouse PCLS challenged with 10−4M methacholine, as lumen area decreased, compressive circumferential strains were highest in the 50 μm closest to the airway lumen while expansive radial strains were highest in the region 50–100 μm from the lumen. However, at any given distance from the airway the strain distribution varied substantially in the vicinity of neighboring small airways and blood vessels. Upon challenge with the relaxant agonist chloroquine, although most strains disappeared, residual positive strains remained a long time after addition of chloroquine, predominantly in the radial direction. Taken together, these findings establish strain mapping as a new tool to elucidate local dynamic mechanical events within the constricting airway and its supporting parenchyma. |
| first_indexed | 2025-11-14T19:53:33Z |
| format | Article |
| id | nottingham-43885 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:53:33Z |
| publishDate | 2016 |
| publisher | Frontiers Media |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-438852020-05-04T18:00:10Z https://eprints.nottingham.ac.uk/43885/ Airway and parenchymal strains during bronchoconstriction in the precision cut lung slice Hiorns, Jonathan E. Bidan, Cécile M. Jensen, Oliver E. Gosens, Reinoud Kistemaker, Loes E.M. Fredberg, Jeffrey J. Butler, Jim P. Krishnan, Ramaswamy Brook, Bindi S. The precision-cut lung slice (PCLS) is a powerful tool for studying airway reactivity, but biomechanical measurements to date have largely focused on changes in airway caliber. Here we describe an image processing tool that reveals the associated spatio-temporal changes in airway and parenchymal strains. Displacements of sub-regions within the PCLS are tracked in phase-contrast movies acquired after addition of contractile and relaxing drugs. From displacement maps, strains are determined across the entire PCLS or along user-specified directions. In a representative mouse PCLS challenged with 10−4M methacholine, as lumen area decreased, compressive circumferential strains were highest in the 50 μm closest to the airway lumen while expansive radial strains were highest in the region 50–100 μm from the lumen. However, at any given distance from the airway the strain distribution varied substantially in the vicinity of neighboring small airways and blood vessels. Upon challenge with the relaxant agonist chloroquine, although most strains disappeared, residual positive strains remained a long time after addition of chloroquine, predominantly in the radial direction. Taken together, these findings establish strain mapping as a new tool to elucidate local dynamic mechanical events within the constricting airway and its supporting parenchyma. Frontiers Media 2016-07-21 Article PeerReviewed Hiorns, Jonathan E., Bidan, Cécile M., Jensen, Oliver E., Gosens, Reinoud, Kistemaker, Loes E.M., Fredberg, Jeffrey J., Butler, Jim P., Krishnan, Ramaswamy and Brook, Bindi S. (2016) Airway and parenchymal strains during bronchoconstriction in the precision cut lung slice. Frontiers in Physiology, 7 . 309/1-309/11. ISSN 1664-042X Airway smooth muscle Contraction PCLS Displacements Radial strain Circumferential strain http://journal.frontiersin.org/article/10.3389/fphys.2016.00309/full doi:10.3389/fphys.2016.00309 doi:10.3389/fphys.2016.00309 |
| spellingShingle | Airway smooth muscle Contraction PCLS Displacements Radial strain Circumferential strain Hiorns, Jonathan E. Bidan, Cécile M. Jensen, Oliver E. Gosens, Reinoud Kistemaker, Loes E.M. Fredberg, Jeffrey J. Butler, Jim P. Krishnan, Ramaswamy Brook, Bindi S. Airway and parenchymal strains during bronchoconstriction in the precision cut lung slice |
| title | Airway and parenchymal strains during bronchoconstriction in the precision cut lung slice |
| title_full | Airway and parenchymal strains during bronchoconstriction in the precision cut lung slice |
| title_fullStr | Airway and parenchymal strains during bronchoconstriction in the precision cut lung slice |
| title_full_unstemmed | Airway and parenchymal strains during bronchoconstriction in the precision cut lung slice |
| title_short | Airway and parenchymal strains during bronchoconstriction in the precision cut lung slice |
| title_sort | airway and parenchymal strains during bronchoconstriction in the precision cut lung slice |
| topic | Airway smooth muscle Contraction PCLS Displacements Radial strain Circumferential strain |
| url | https://eprints.nottingham.ac.uk/43885/ https://eprints.nottingham.ac.uk/43885/ https://eprints.nottingham.ac.uk/43885/ |