Complete sequence and genomic annotation of carrot torradovirus 1

Carrot torradovirus 1 (CaTV1) is a new member of the genus Torradovirus within the family Secoviridae. CaTV1 genome sequences were obtained from a previous next-generation sequencing (NGS) study and were compared to other members and tentative new members of the genus. The virus has a bipartite geno...

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Main Authors: Rozado-Aguirre, Zuriñe, Adams, Ian, Fox, Adrian, Dickinson, Matthew, Boonham, Neil
Format: Article
Published: Springer 2017
Online Access:https://eprints.nottingham.ac.uk/43729/
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author Rozado-Aguirre, Zuriñe
Adams, Ian
Fox, Adrian
Dickinson, Matthew
Boonham, Neil
author_facet Rozado-Aguirre, Zuriñe
Adams, Ian
Fox, Adrian
Dickinson, Matthew
Boonham, Neil
author_sort Rozado-Aguirre, Zuriñe
building Nottingham Research Data Repository
collection Online Access
description Carrot torradovirus 1 (CaTV1) is a new member of the genus Torradovirus within the family Secoviridae. CaTV1 genome sequences were obtained from a previous next-generation sequencing (NGS) study and were compared to other members and tentative new members of the genus. The virus has a bipartite genome, and RACE was used to amplify and sequence each end of RNA1 and RNA2. As a result, RNA1 and RNA2 are estimated to contain 6944 and 4995 nucleotides, respectively, with RNA1 encoding the proteins involved in virus replication, and RNA2 encoding the encapsidation and movement proteins. Sequence comparisons showed that CaTV1 clustered within the non-tomato-infecting torradoviruses and is most similar to motherwort yellow mottle virus (MYMoV). The nucleotide sequence identities of the Pro-Pol and coat protein regions were below the criteria established by the ICTV for demarcating species, confirming that CaTV1 should be classified as a member of a new species within the genus Torradovirus.
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spelling nottingham-437292020-05-04T18:46:21Z https://eprints.nottingham.ac.uk/43729/ Complete sequence and genomic annotation of carrot torradovirus 1 Rozado-Aguirre, Zuriñe Adams, Ian Fox, Adrian Dickinson, Matthew Boonham, Neil Carrot torradovirus 1 (CaTV1) is a new member of the genus Torradovirus within the family Secoviridae. CaTV1 genome sequences were obtained from a previous next-generation sequencing (NGS) study and were compared to other members and tentative new members of the genus. The virus has a bipartite genome, and RACE was used to amplify and sequence each end of RNA1 and RNA2. As a result, RNA1 and RNA2 are estimated to contain 6944 and 4995 nucleotides, respectively, with RNA1 encoding the proteins involved in virus replication, and RNA2 encoding the encapsidation and movement proteins. Sequence comparisons showed that CaTV1 clustered within the non-tomato-infecting torradoviruses and is most similar to motherwort yellow mottle virus (MYMoV). The nucleotide sequence identities of the Pro-Pol and coat protein regions were below the criteria established by the ICTV for demarcating species, confirming that CaTV1 should be classified as a member of a new species within the genus Torradovirus. Springer 2017-05-19 Article PeerReviewed Rozado-Aguirre, Zuriñe, Adams, Ian, Fox, Adrian, Dickinson, Matthew and Boonham, Neil (2017) Complete sequence and genomic annotation of carrot torradovirus 1. Archives of Virology . ISSN 0304-8608 https://doi.org/10.1007/s00705-017-3410-5 doi:10.1007/s00705-017-3410-5 doi:10.1007/s00705-017-3410-5
spellingShingle Rozado-Aguirre, Zuriñe
Adams, Ian
Fox, Adrian
Dickinson, Matthew
Boonham, Neil
Complete sequence and genomic annotation of carrot torradovirus 1
title Complete sequence and genomic annotation of carrot torradovirus 1
title_full Complete sequence and genomic annotation of carrot torradovirus 1
title_fullStr Complete sequence and genomic annotation of carrot torradovirus 1
title_full_unstemmed Complete sequence and genomic annotation of carrot torradovirus 1
title_short Complete sequence and genomic annotation of carrot torradovirus 1
title_sort complete sequence and genomic annotation of carrot torradovirus 1
url https://eprints.nottingham.ac.uk/43729/
https://eprints.nottingham.ac.uk/43729/
https://eprints.nottingham.ac.uk/43729/