Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro
Prion diseases are fatal and incurable neurodegenerative diseases of humans and animals. Despite years of research, no therapeutic agents have been developed that can effectively manage or reverse disease progression. Recently it has been identified that recombinant prion proteins (rPrP) expressed i...
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| Format: | Article |
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Taylor & Francis
2017
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| Online Access: | https://eprints.nottingham.ac.uk/43681/ |
| _version_ | 1848796743309721600 |
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| author | Maddison, Ben C. Workman, Robert G. Gough, Kevin C. |
| author_facet | Maddison, Ben C. Workman, Robert G. Gough, Kevin C. |
| author_sort | Maddison, Ben C. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Prion diseases are fatal and incurable neurodegenerative diseases of humans and animals. Despite years of research, no therapeutic agents have been developed that can effectively manage or reverse disease progression. Recently it has been identified that recombinant prion proteins (rPrP) expressed in bacteria can act as inhibitors of prion replication within the in vitro prion replication system Protein Misfolding Cyclic Amplification (PMCA). Here, within PMCA reactions amplifying a range of ruminant prions including distinct Prnp genotypes/host species and distinct prion strains, recombinant ovine VRQ PrP displayed consistent inhibition of prion replication and produced IC50 values of 122 and 171 nM for ovine scrapie and bovine BSE replication, respectively. These findings illustrate the therapeutic potential of rPrPs with distinct TSE diseases. |
| first_indexed | 2025-11-14T19:52:50Z |
| format | Article |
| id | nottingham-43681 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:52:50Z |
| publishDate | 2017 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-436812020-05-04T18:53:22Z https://eprints.nottingham.ac.uk/43681/ Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro Maddison, Ben C. Workman, Robert G. Gough, Kevin C. Prion diseases are fatal and incurable neurodegenerative diseases of humans and animals. Despite years of research, no therapeutic agents have been developed that can effectively manage or reverse disease progression. Recently it has been identified that recombinant prion proteins (rPrP) expressed in bacteria can act as inhibitors of prion replication within the in vitro prion replication system Protein Misfolding Cyclic Amplification (PMCA). Here, within PMCA reactions amplifying a range of ruminant prions including distinct Prnp genotypes/host species and distinct prion strains, recombinant ovine VRQ PrP displayed consistent inhibition of prion replication and produced IC50 values of 122 and 171 nM for ovine scrapie and bovine BSE replication, respectively. These findings illustrate the therapeutic potential of rPrPs with distinct TSE diseases. Taylor & Francis 2017-06-30 Article PeerReviewed Maddison, Ben C., Workman, Robert G. and Gough, Kevin C. (2017) Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro. Prion, 11 (4). pp. 265-276. ISSN 1933-690X Scrapie BSE Recombinant PrP prion diseases PMCA Protein inhibitors Therapeutics http://www.tandfonline.com/doi/full/10.1080/19336896.2017.1342919 doi:10.1080/19336896.2017.1342919 doi:10.1080/19336896.2017.1342919 |
| spellingShingle | Scrapie BSE Recombinant PrP prion diseases PMCA Protein inhibitors Therapeutics Maddison, Ben C. Workman, Robert G. Gough, Kevin C. Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro |
| title | Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro |
| title_full | Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro |
| title_fullStr | Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro |
| title_full_unstemmed | Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro |
| title_short | Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro |
| title_sort | ovine recombinant prp as an inhibitor of ruminant prion propagation in vitro |
| topic | Scrapie BSE Recombinant PrP prion diseases PMCA Protein inhibitors Therapeutics |
| url | https://eprints.nottingham.ac.uk/43681/ https://eprints.nottingham.ac.uk/43681/ https://eprints.nottingham.ac.uk/43681/ |