Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging
Diffusion MRI is an exquisitely sensitive probe of tissue microstructure, and is currently the only non-invasive measure of the brain’s fibre architecture. As this technique becomes more sophisticated and microstructurally informative, there is increasing value in comparing diffusion MRI with micros...
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Elsevier
2017
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| Online Access: | https://eprints.nottingham.ac.uk/43569/ |
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| author | Mollink, Jeroen Kleinnijenhuis, Michiel Cappellen van Walsum, Anne-Marie van Sotiropoulos, Stamatios N. Cottaar, Michiel Mirfin, Christopher Heinrich, Mattias P. Jenkinson, Mark Pallebage-Gamarallage, Menuka Ansorge, Olaf Jbabdi, Saad Miller, Karla L. |
| author_facet | Mollink, Jeroen Kleinnijenhuis, Michiel Cappellen van Walsum, Anne-Marie van Sotiropoulos, Stamatios N. Cottaar, Michiel Mirfin, Christopher Heinrich, Mattias P. Jenkinson, Mark Pallebage-Gamarallage, Menuka Ansorge, Olaf Jbabdi, Saad Miller, Karla L. |
| author_sort | Mollink, Jeroen |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Diffusion MRI is an exquisitely sensitive probe of tissue microstructure, and is currently the only non-invasive measure of the brain’s fibre architecture. As this technique becomes more sophisticated and microstructurally informative, there is increasing value in comparing diffusion MRI with microscopic imaging in the same tissue samples. This study compared estimates of fibre orientation dispersion in white matter derived from diffusion MRI to reference measures of dispersion obtained from polarized light imaging and histology.
Three post-mortem brain specimens were scanned with diffusion MRI and analyzed with a two-compartment dispersion model. The specimens were then sectioned for microscopy, including polarized light imaging estimates of fibre orientation and histological quantitative estimates of myelin and astrocytes. Dispersion estimates were correlated on region – and voxel-wise levels in the corpus callosum, the centrum semiovale and the corticospinal tract.
The region-wise analysis yielded correlation coefficients of r=0.79 for the diffusion MRI and histology comparison, while r=0.60 was reported for the comparison with polarized light imaging. In the corpus callosum, we observed a pattern of higher dispersion at the midline compared to its lateral aspects. This pattern was present in all modalities and the dispersion profiles from microscopy and diffusion MRI were highly correlated. The astrocytes appeared to have minor contribution to dispersion observed with diffusion MRI.
These results demonstrate that fibre orientation dispersion estimates from diffusion MRI represents the tissue architecture well. Dispersion models might be improved by more faithfully incorporating an informed mapping based on microscopy data. |
| first_indexed | 2025-11-14T19:52:25Z |
| format | Article |
| id | nottingham-43569 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:52:25Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-435692020-05-04T19:00:55Z https://eprints.nottingham.ac.uk/43569/ Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging Mollink, Jeroen Kleinnijenhuis, Michiel Cappellen van Walsum, Anne-Marie van Sotiropoulos, Stamatios N. Cottaar, Michiel Mirfin, Christopher Heinrich, Mattias P. Jenkinson, Mark Pallebage-Gamarallage, Menuka Ansorge, Olaf Jbabdi, Saad Miller, Karla L. Diffusion MRI is an exquisitely sensitive probe of tissue microstructure, and is currently the only non-invasive measure of the brain’s fibre architecture. As this technique becomes more sophisticated and microstructurally informative, there is increasing value in comparing diffusion MRI with microscopic imaging in the same tissue samples. This study compared estimates of fibre orientation dispersion in white matter derived from diffusion MRI to reference measures of dispersion obtained from polarized light imaging and histology. Three post-mortem brain specimens were scanned with diffusion MRI and analyzed with a two-compartment dispersion model. The specimens were then sectioned for microscopy, including polarized light imaging estimates of fibre orientation and histological quantitative estimates of myelin and astrocytes. Dispersion estimates were correlated on region – and voxel-wise levels in the corpus callosum, the centrum semiovale and the corticospinal tract. The region-wise analysis yielded correlation coefficients of r=0.79 for the diffusion MRI and histology comparison, while r=0.60 was reported for the comparison with polarized light imaging. In the corpus callosum, we observed a pattern of higher dispersion at the midline compared to its lateral aspects. This pattern was present in all modalities and the dispersion profiles from microscopy and diffusion MRI were highly correlated. The astrocytes appeared to have minor contribution to dispersion observed with diffusion MRI. These results demonstrate that fibre orientation dispersion estimates from diffusion MRI represents the tissue architecture well. Dispersion models might be improved by more faithfully incorporating an informed mapping based on microscopy data. Elsevier 2017-08-15 Article PeerReviewed Mollink, Jeroen, Kleinnijenhuis, Michiel, Cappellen van Walsum, Anne-Marie van, Sotiropoulos, Stamatios N., Cottaar, Michiel, Mirfin, Christopher, Heinrich, Mattias P., Jenkinson, Mark, Pallebage-Gamarallage, Menuka, Ansorge, Olaf, Jbabdi, Saad and Miller, Karla L. (2017) Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging. NeuroImage, 157 . pp. 561-574. ISSN 1095-9572 Dispersion; Diffusion MRI; Post-mortem; Polarized Light Imaging; Myelin; Astrocytes; Validation http://www.sciencedirect.com/science/article/pii/S1053811917304706 doi:10.1016/j.neuroimage.2017.06.001 doi:10.1016/j.neuroimage.2017.06.001 |
| spellingShingle | Dispersion; Diffusion MRI; Post-mortem; Polarized Light Imaging; Myelin; Astrocytes; Validation Mollink, Jeroen Kleinnijenhuis, Michiel Cappellen van Walsum, Anne-Marie van Sotiropoulos, Stamatios N. Cottaar, Michiel Mirfin, Christopher Heinrich, Mattias P. Jenkinson, Mark Pallebage-Gamarallage, Menuka Ansorge, Olaf Jbabdi, Saad Miller, Karla L. Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging |
| title | Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging |
| title_full | Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging |
| title_fullStr | Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging |
| title_full_unstemmed | Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging |
| title_short | Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging |
| title_sort | evaluating fibre orientation dispersion in white matter: comparison of diffusion mri, histology and polarized light imaging |
| topic | Dispersion; Diffusion MRI; Post-mortem; Polarized Light Imaging; Myelin; Astrocytes; Validation |
| url | https://eprints.nottingham.ac.uk/43569/ https://eprints.nottingham.ac.uk/43569/ https://eprints.nottingham.ac.uk/43569/ |