Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea
Context: There is an unmet need to discover new treatments for Alzheimer’s disease. This study determined the anti-acetylcholinesterase (AChE) activity, DPPH free radical scavenging and antioxidant properties of Carpolobia lutea G. Don (Polygalaceae). Objective: To quantify C. lutea anti-AChE, DPPH...
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| Format: | Article |
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Taylor & Francis
2017
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| Online Access: | https://eprints.nottingham.ac.uk/43529/ |
| _version_ | 1848796706506801152 |
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| author | Nwidu, Lucky Legbosi Elmorsy, Ekramy Thornton, Jack Wijamunige, Buddhika Wijesekara, Anusha Tarbox, Rebecca Warren, Averil Carter, Wayne |
| author_facet | Nwidu, Lucky Legbosi Elmorsy, Ekramy Thornton, Jack Wijamunige, Buddhika Wijesekara, Anusha Tarbox, Rebecca Warren, Averil Carter, Wayne |
| author_sort | Nwidu, Lucky Legbosi |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Context: There is an unmet need to discover new treatments for Alzheimer’s disease. This study determined the anti-acetylcholinesterase (AChE) activity, DPPH free radical scavenging and antioxidant properties of Carpolobia lutea G. Don (Polygalaceae).
Objective: To quantify C. lutea anti-AChE, DPPH free radical scavenging, and antioxidant activities, and cell cytotoxicity.
Materials and methods: Plant stem, leaves, and roots were subjected to sequential solvent extractions, and screened for anti-AChE activity across a concentration range of 0.02-200 μg//mL. Plant DPPH radical scavenging activity, reducing power, and total phenolic and flavonoid content were determined, and cytotoxicity evaluated using human hepatocytes.
Results: C. lutea exhibited concentration dependent anti-AChE activity. The most potent inhibitory activity for the stem was the crude ethanol extract and hexane stem fraction oil (IC₅₀ = 140 μg/mL); for the leaves the chloroform leaf fraction (IC₅₀ = 60 μg/mL/mL); and for roots, the methanol, ethyl acetate, and aqueous root fractions (IC₅₀ = 0.3-3 μg/mL). Dose-dependent free radical scavenging activity and reducing power were observed with increasing stem, leaf, or root concentration. Total phenolics were highest in the stem: ~632 mg gallic acid equivalents/g for a hexane stem fraction oil. Total flavonoid content was highest in the leaves: ~297 mg quercetin equivalents/g for a chloroform leaf fraction. At 1 μg/mL, only the crude ethanol extract oil was significantly cytotoxic to hepatocytes.
Discussion and conclusion: C. lutea possesses anti-AChE activity and beneficial antioxidant capacity indicative of its potential development as a treatment of Alzheimer’s and other diseases characterized by a cholinergic deficit. |
| first_indexed | 2025-11-14T19:52:15Z |
| format | Article |
| id | nottingham-43529 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:52:15Z |
| publishDate | 2017 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-435292020-05-04T18:50:47Z https://eprints.nottingham.ac.uk/43529/ Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea Nwidu, Lucky Legbosi Elmorsy, Ekramy Thornton, Jack Wijamunige, Buddhika Wijesekara, Anusha Tarbox, Rebecca Warren, Averil Carter, Wayne Context: There is an unmet need to discover new treatments for Alzheimer’s disease. This study determined the anti-acetylcholinesterase (AChE) activity, DPPH free radical scavenging and antioxidant properties of Carpolobia lutea G. Don (Polygalaceae). Objective: To quantify C. lutea anti-AChE, DPPH free radical scavenging, and antioxidant activities, and cell cytotoxicity. Materials and methods: Plant stem, leaves, and roots were subjected to sequential solvent extractions, and screened for anti-AChE activity across a concentration range of 0.02-200 μg//mL. Plant DPPH radical scavenging activity, reducing power, and total phenolic and flavonoid content were determined, and cytotoxicity evaluated using human hepatocytes. Results: C. lutea exhibited concentration dependent anti-AChE activity. The most potent inhibitory activity for the stem was the crude ethanol extract and hexane stem fraction oil (IC₅₀ = 140 μg/mL); for the leaves the chloroform leaf fraction (IC₅₀ = 60 μg/mL/mL); and for roots, the methanol, ethyl acetate, and aqueous root fractions (IC₅₀ = 0.3-3 μg/mL). Dose-dependent free radical scavenging activity and reducing power were observed with increasing stem, leaf, or root concentration. Total phenolics were highest in the stem: ~632 mg gallic acid equivalents/g for a hexane stem fraction oil. Total flavonoid content was highest in the leaves: ~297 mg quercetin equivalents/g for a chloroform leaf fraction. At 1 μg/mL, only the crude ethanol extract oil was significantly cytotoxic to hepatocytes. Discussion and conclusion: C. lutea possesses anti-AChE activity and beneficial antioxidant capacity indicative of its potential development as a treatment of Alzheimer’s and other diseases characterized by a cholinergic deficit. Taylor & Francis 2017-06-19 Article PeerReviewed Nwidu, Lucky Legbosi, Elmorsy, Ekramy, Thornton, Jack, Wijamunige, Buddhika, Wijesekara, Anusha, Tarbox, Rebecca, Warren, Averil and Carter, Wayne (2017) Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea. Pharmaceutical Biology, 55 (1). pp. 1875-1883. ISSN 1744-5116 Anti-acetylcholinesterase; Carpolobia lutea; Antioxidant; Alzheimer's disease https://www.tandfonline.com/doi/full/10.1080/13880209.2017.1339283 doi:10.1080/13880209.2017.1339283 doi:10.1080/13880209.2017.1339283 |
| spellingShingle | Anti-acetylcholinesterase; Carpolobia lutea; Antioxidant; Alzheimer's disease Nwidu, Lucky Legbosi Elmorsy, Ekramy Thornton, Jack Wijamunige, Buddhika Wijesekara, Anusha Tarbox, Rebecca Warren, Averil Carter, Wayne Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea |
| title | Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea |
| title_full | Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea |
| title_fullStr | Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea |
| title_full_unstemmed | Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea |
| title_short | Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea |
| title_sort | anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of carpolobia lutea |
| topic | Anti-acetylcholinesterase; Carpolobia lutea; Antioxidant; Alzheimer's disease |
| url | https://eprints.nottingham.ac.uk/43529/ https://eprints.nottingham.ac.uk/43529/ https://eprints.nottingham.ac.uk/43529/ |