A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study
BACKGROUND: Currently available diagnostic techniques of Plasmodium falciparum infection are not optimal for non-invasive, population-based screening for malaria. It was hypothesized that a mass spectrometry-based metabolomics approach could identify urinary biomarkers of falciparum malaria. MET...
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| Format: | Article |
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BioMed Central
2017
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| Online Access: | https://eprints.nottingham.ac.uk/43401/ |
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| author | Abdelrazig, Salah M.A. Ortori, Catharine A. Davey, Gail Deressa, Wakgari Mulleta, Dhaba Barrett, David A. Amberbir, Alemayehu Fogarty, Andrew W. |
| author_facet | Abdelrazig, Salah M.A. Ortori, Catharine A. Davey, Gail Deressa, Wakgari Mulleta, Dhaba Barrett, David A. Amberbir, Alemayehu Fogarty, Andrew W. |
| author_sort | Abdelrazig, Salah M.A. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | BACKGROUND: Currently available diagnostic techniques of Plasmodium falciparum infection are not optimal for non-invasive, population-based screening for malaria. It was hypothesized that a mass spectrometry-based metabolomics approach could identify urinary biomarkers of falciparum malaria.
METHODS: The study used a case-control design, with cases consisting of 21 adults in central Ethiopia with a diagnosis of P. falciparum infection confirmed with microscopy, and 25 controls of adults with negative blood smears for malaria matched on age and sex. Urinary samples were collected from these individuals during presentation at the clinic, and a second sample was collected from both cases and controls 4 weeks later, after the cases had received anti-malarial medication. The urine samples were screened for small molecule urinary biomarkers, using mass spectrometry-based metabolomics analyses followed by multivariate analysis using principal component analysis and orthogonal partial least square-discriminant analysis. The chemical identity of statistically significant malaria biomarkers was confirmed using tandem mass spectrometry.
RESULTS: The urinary metabolic profiles of cases with P. falciparum infection were distinct from healthy controls. After treatment with anti-malarial medication, the metabolomic profile of cases resembled that of healthy controls. Significantly altered levels of 29 urinary metabolites were found. Elevated levels of urinary pipecolic acid, taurine, N-acetylspermidine, N-acetylputrescine and 1,3-diacetylpropane were identified as potential biomarkers of falciparum malaria.
CONCLUSION: The urinary biomarkers of malaria identified have potential for the development of non-invasive and rapid diagnostic test of P. falciparum infection. |
| first_indexed | 2025-11-14T19:51:49Z |
| format | Article |
| id | nottingham-43401 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:51:49Z |
| publishDate | 2017 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-434012020-05-04T18:47:16Z https://eprints.nottingham.ac.uk/43401/ A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study Abdelrazig, Salah M.A. Ortori, Catharine A. Davey, Gail Deressa, Wakgari Mulleta, Dhaba Barrett, David A. Amberbir, Alemayehu Fogarty, Andrew W. BACKGROUND: Currently available diagnostic techniques of Plasmodium falciparum infection are not optimal for non-invasive, population-based screening for malaria. It was hypothesized that a mass spectrometry-based metabolomics approach could identify urinary biomarkers of falciparum malaria. METHODS: The study used a case-control design, with cases consisting of 21 adults in central Ethiopia with a diagnosis of P. falciparum infection confirmed with microscopy, and 25 controls of adults with negative blood smears for malaria matched on age and sex. Urinary samples were collected from these individuals during presentation at the clinic, and a second sample was collected from both cases and controls 4 weeks later, after the cases had received anti-malarial medication. The urine samples were screened for small molecule urinary biomarkers, using mass spectrometry-based metabolomics analyses followed by multivariate analysis using principal component analysis and orthogonal partial least square-discriminant analysis. The chemical identity of statistically significant malaria biomarkers was confirmed using tandem mass spectrometry. RESULTS: The urinary metabolic profiles of cases with P. falciparum infection were distinct from healthy controls. After treatment with anti-malarial medication, the metabolomic profile of cases resembled that of healthy controls. Significantly altered levels of 29 urinary metabolites were found. Elevated levels of urinary pipecolic acid, taurine, N-acetylspermidine, N-acetylputrescine and 1,3-diacetylpropane were identified as potential biomarkers of falciparum malaria. CONCLUSION: The urinary biomarkers of malaria identified have potential for the development of non-invasive and rapid diagnostic test of P. falciparum infection. BioMed Central 2017-05-30 Article PeerReviewed Abdelrazig, Salah M.A., Ortori, Catharine A., Davey, Gail, Deressa, Wakgari, Mulleta, Dhaba, Barrett, David A., Amberbir, Alemayehu and Fogarty, Andrew W. (2017) A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study. Malaria Journal, 16 . 229/1-229/8. ISSN 1475-2875 Malaria Urine Falciparum Biomarker Metabolomics http://malariajournal.biomedcentral.com/articles/10.1186/s12936-017-1875-z doi:10.1186/s12936-017-1875-z doi:10.1186/s12936-017-1875-z |
| spellingShingle | Malaria Urine Falciparum Biomarker Metabolomics Abdelrazig, Salah M.A. Ortori, Catharine A. Davey, Gail Deressa, Wakgari Mulleta, Dhaba Barrett, David A. Amberbir, Alemayehu Fogarty, Andrew W. A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study |
| title | A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study |
| title_full | A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study |
| title_fullStr | A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study |
| title_full_unstemmed | A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study |
| title_short | A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study |
| title_sort | metabolomic analytical approach permits identification of urinary biomarkers for plasmodium falciparum infection: a case–control study |
| topic | Malaria Urine Falciparum Biomarker Metabolomics |
| url | https://eprints.nottingham.ac.uk/43401/ https://eprints.nottingham.ac.uk/43401/ https://eprints.nottingham.ac.uk/43401/ |