Proteomic differences between developmental stages of Toxoplasma gondii revealed by iTRAQ-based quantitative proteomics
Toxoplasma gondii has a complex two-host life-cycle between intermediate host and definitive host. Understanding proteomic variations across the life-cycle stages of T. gondii may improve the understanding of molecular adaption mechanism of T. gondii across life-cycle stages, and should have implica...
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| Format: | Article |
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Frontiers
2017
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| Online Access: | https://eprints.nottingham.ac.uk/43365/ |
| _version_ | 1848796671536791552 |
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| author | Wang, Ze-Xiang Zhou, Chun-Xue Elsheikha, Hany M. He, Shuai Zhou, Dong-Hui Zhu, Xing-Quan |
| author_facet | Wang, Ze-Xiang Zhou, Chun-Xue Elsheikha, Hany M. He, Shuai Zhou, Dong-Hui Zhu, Xing-Quan |
| author_sort | Wang, Ze-Xiang |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Toxoplasma gondii has a complex two-host life-cycle between intermediate host and definitive host. Understanding proteomic variations across the life-cycle stages of T. gondii may improve the understanding of molecular adaption mechanism of T. gondii across life-cycle stages, and should have implications for the development of new treatment and prevention interventions against T. gondii infection. Here, we utilized LC–MS/MS coupled with iTRAQ labeling technology to identify differentially expressed proteins (DEPs) specific to tachyzoite (T), bradyzoites-containing cyst (C) and sporulated oocyst (O) stages of the cyst-forming T. gondii Prugniuad (Pru) strain. A total of 6285 proteins were identified in the three developmental stages of T. gondii. Our analysis also revealed 875, 656, and 538 DEPs in O vs. T, T vs. C, and C vs. O, respectively. The up- and down-regulated proteins were analyzed by Gene Ontology enrichment, KEGG pathway and STRING analyses. Some virulence-related factors and ribosomal proteins exhibited distinct expression patterns across the life-cycle stages. The virulence factors expressed in sporulated oocysts and the number of up-regulated virulence factors in the cyst stage were about twice as many as in tachyzoites. Of the 79 ribosomal proteins identified in T. gondii, the number of up-regulated ribosomal proteins was 33 and 46 in sporulated oocysts and cysts, respectively, compared with tachyzoites. These results support the hypothesis that oocyst and cystic stages are able to adapt to adverse environmental conditions and selection pressures induced by the host’s immune response, respectively. These findings have important implications for understanding of the developmental biology of T. gondii, which may facilitate the discovery of novel therapeutic targets to better control toxoplasmosis. |
| first_indexed | 2025-11-14T19:51:41Z |
| format | Article |
| id | nottingham-43365 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:51:41Z |
| publishDate | 2017 |
| publisher | Frontiers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-433652020-05-04T18:48:45Z https://eprints.nottingham.ac.uk/43365/ Proteomic differences between developmental stages of Toxoplasma gondii revealed by iTRAQ-based quantitative proteomics Wang, Ze-Xiang Zhou, Chun-Xue Elsheikha, Hany M. He, Shuai Zhou, Dong-Hui Zhu, Xing-Quan Toxoplasma gondii has a complex two-host life-cycle between intermediate host and definitive host. Understanding proteomic variations across the life-cycle stages of T. gondii may improve the understanding of molecular adaption mechanism of T. gondii across life-cycle stages, and should have implications for the development of new treatment and prevention interventions against T. gondii infection. Here, we utilized LC–MS/MS coupled with iTRAQ labeling technology to identify differentially expressed proteins (DEPs) specific to tachyzoite (T), bradyzoites-containing cyst (C) and sporulated oocyst (O) stages of the cyst-forming T. gondii Prugniuad (Pru) strain. A total of 6285 proteins were identified in the three developmental stages of T. gondii. Our analysis also revealed 875, 656, and 538 DEPs in O vs. T, T vs. C, and C vs. O, respectively. The up- and down-regulated proteins were analyzed by Gene Ontology enrichment, KEGG pathway and STRING analyses. Some virulence-related factors and ribosomal proteins exhibited distinct expression patterns across the life-cycle stages. The virulence factors expressed in sporulated oocysts and the number of up-regulated virulence factors in the cyst stage were about twice as many as in tachyzoites. Of the 79 ribosomal proteins identified in T. gondii, the number of up-regulated ribosomal proteins was 33 and 46 in sporulated oocysts and cysts, respectively, compared with tachyzoites. These results support the hypothesis that oocyst and cystic stages are able to adapt to adverse environmental conditions and selection pressures induced by the host’s immune response, respectively. These findings have important implications for understanding of the developmental biology of T. gondii, which may facilitate the discovery of novel therapeutic targets to better control toxoplasmosis. Frontiers 2017-06-02 Article PeerReviewed Wang, Ze-Xiang, Zhou, Chun-Xue, Elsheikha, Hany M., He, Shuai, Zhou, Dong-Hui and Zhu, Xing-Quan (2017) Proteomic differences between developmental stages of Toxoplasma gondii revealed by iTRAQ-based quantitative proteomics. Frontiers in Microbiology, 8 . ISSN 1664-302X http://journal.frontiersin.org/article/10.3389/fmicb.2017.00985/full doi:10.3389/fmicb.2017.00985 doi:10.3389/fmicb.2017.00985 |
| spellingShingle | Wang, Ze-Xiang Zhou, Chun-Xue Elsheikha, Hany M. He, Shuai Zhou, Dong-Hui Zhu, Xing-Quan Proteomic differences between developmental stages of Toxoplasma gondii revealed by iTRAQ-based quantitative proteomics |
| title | Proteomic differences between developmental stages of Toxoplasma gondii revealed by iTRAQ-based quantitative proteomics |
| title_full | Proteomic differences between developmental stages of Toxoplasma gondii revealed by iTRAQ-based quantitative proteomics |
| title_fullStr | Proteomic differences between developmental stages of Toxoplasma gondii revealed by iTRAQ-based quantitative proteomics |
| title_full_unstemmed | Proteomic differences between developmental stages of Toxoplasma gondii revealed by iTRAQ-based quantitative proteomics |
| title_short | Proteomic differences between developmental stages of Toxoplasma gondii revealed by iTRAQ-based quantitative proteomics |
| title_sort | proteomic differences between developmental stages of toxoplasma gondii revealed by itraq-based quantitative proteomics |
| url | https://eprints.nottingham.ac.uk/43365/ https://eprints.nottingham.ac.uk/43365/ https://eprints.nottingham.ac.uk/43365/ |