Activation induced changes in GABA: functional MRS at 7 T with MEGA-sLASER

Functional magnetic resonance spectroscopy (fMRS) has been used to assess the dynamic metabolic responses of the brain to a physiological stimulus non-invasively. However, only limited information on the dynamic functional response of γ-aminobutyric acid (GABA), the primary inhibitory neurotransmitt...

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Main Authors: Chen, Chen, Sigurdsson, Hilmar P., Pépés, Sophia E., Auer, Dorothee P., Morris, Peter G., Morgan, Paul S., Gowland, Penny A., Jackson, Stephen R.
Format: Article
Published: Elsevier 2017
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Online Access:https://eprints.nottingham.ac.uk/43021/
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author Chen, Chen
Sigurdsson, Hilmar P.
Pépés, Sophia E.
Auer, Dorothee P.
Morris, Peter G.
Morgan, Paul S.
Gowland, Penny A.
Jackson, Stephen R.
author_facet Chen, Chen
Sigurdsson, Hilmar P.
Pépés, Sophia E.
Auer, Dorothee P.
Morris, Peter G.
Morgan, Paul S.
Gowland, Penny A.
Jackson, Stephen R.
author_sort Chen, Chen
building Nottingham Research Data Repository
collection Online Access
description Functional magnetic resonance spectroscopy (fMRS) has been used to assess the dynamic metabolic responses of the brain to a physiological stimulus non-invasively. However, only limited information on the dynamic functional response of γ-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the brain, is available. We aimed to measure the activation-induced changes in GABA unambiguously using a spectral editing method, instead of the conventional direct detection techniques used in previous fMRS studies. The Mescher-Garwood-semi-localised by adiabatic selective refocusing (MEGA-sLASER) sequence was developed at 7 T to obtain the time course of GABA concentration without macromolecular contamination. A significant decrease (−12±5%) in the GABA to total creatine ratio (GABA/tCr) was observed in the motor cortex during a period of 10 minutes of hand-clenching, compared to an initial baseline level (GABA/tCr = 0.11±0.02) at rest. An increase in the Glx (glutamate and glutamine) to tCr ratio was also found, which is in agreement with previous findings. In contrast, no significant changes in NAA/tCr and tCr were detected. With consistent and highly efficient editing performance for GABA detection and the advantage of visually identifying GABA resonances in the spectra, MEGA-sLASER is demonstrated to be an effective method for studying of dynamic changes in GABA at 7 T.
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spelling nottingham-430212020-05-04T18:58:26Z https://eprints.nottingham.ac.uk/43021/ Activation induced changes in GABA: functional MRS at 7 T with MEGA-sLASER Chen, Chen Sigurdsson, Hilmar P. Pépés, Sophia E. Auer, Dorothee P. Morris, Peter G. Morgan, Paul S. Gowland, Penny A. Jackson, Stephen R. Functional magnetic resonance spectroscopy (fMRS) has been used to assess the dynamic metabolic responses of the brain to a physiological stimulus non-invasively. However, only limited information on the dynamic functional response of γ-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the brain, is available. We aimed to measure the activation-induced changes in GABA unambiguously using a spectral editing method, instead of the conventional direct detection techniques used in previous fMRS studies. The Mescher-Garwood-semi-localised by adiabatic selective refocusing (MEGA-sLASER) sequence was developed at 7 T to obtain the time course of GABA concentration without macromolecular contamination. A significant decrease (−12±5%) in the GABA to total creatine ratio (GABA/tCr) was observed in the motor cortex during a period of 10 minutes of hand-clenching, compared to an initial baseline level (GABA/tCr = 0.11±0.02) at rest. An increase in the Glx (glutamate and glutamine) to tCr ratio was also found, which is in agreement with previous findings. In contrast, no significant changes in NAA/tCr and tCr were detected. With consistent and highly efficient editing performance for GABA detection and the advantage of visually identifying GABA resonances in the spectra, MEGA-sLASER is demonstrated to be an effective method for studying of dynamic changes in GABA at 7 T. Elsevier 2017-08-01 Article PeerReviewed Chen, Chen, Sigurdsson, Hilmar P., Pépés, Sophia E., Auer, Dorothee P., Morris, Peter G., Morgan, Paul S., Gowland, Penny A. and Jackson, Stephen R. (2017) Activation induced changes in GABA: functional MRS at 7 T with MEGA-sLASER. NeuroImage, 156 . pp. 207-213. ISSN 1095-9572 GABA; MEGA editing; functional MRS; motor cortex; 7T http://www.sciencedirect.com/science/article/pii/S1053811917304433 doi:10.1016/j.neuroimage.2017.05.044 doi:10.1016/j.neuroimage.2017.05.044
spellingShingle GABA; MEGA editing; functional MRS; motor cortex; 7T
Chen, Chen
Sigurdsson, Hilmar P.
Pépés, Sophia E.
Auer, Dorothee P.
Morris, Peter G.
Morgan, Paul S.
Gowland, Penny A.
Jackson, Stephen R.
Activation induced changes in GABA: functional MRS at 7 T with MEGA-sLASER
title Activation induced changes in GABA: functional MRS at 7 T with MEGA-sLASER
title_full Activation induced changes in GABA: functional MRS at 7 T with MEGA-sLASER
title_fullStr Activation induced changes in GABA: functional MRS at 7 T with MEGA-sLASER
title_full_unstemmed Activation induced changes in GABA: functional MRS at 7 T with MEGA-sLASER
title_short Activation induced changes in GABA: functional MRS at 7 T with MEGA-sLASER
title_sort activation induced changes in gaba: functional mrs at 7 t with mega-slaser
topic GABA; MEGA editing; functional MRS; motor cortex; 7T
url https://eprints.nottingham.ac.uk/43021/
https://eprints.nottingham.ac.uk/43021/
https://eprints.nottingham.ac.uk/43021/