Multiboronic acid-conjugated chitosan scaffolds with glucose selectivity to insulin release

The principal challenge for the use of boronic acids (BA) as glucose sensors is their lack of specificity for glucose. We examined the selectivity of and insulin release from two boronic acids- (2-formyl-3-thienylboronic acid (FTBA) and 4-formylphenylboronic acid (FPBA)) conjugated chitosan scaffold...

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Main Authors: Siddiqui, Nabil A., Billa, Nashiru, Roberts, Clive J.
Format: Article
Published: Taylor & Francis 2017
Subjects:
Online Access:https://eprints.nottingham.ac.uk/43003/
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author Siddiqui, Nabil A.
Billa, Nashiru
Roberts, Clive J.
author_facet Siddiqui, Nabil A.
Billa, Nashiru
Roberts, Clive J.
author_sort Siddiqui, Nabil A.
building Nottingham Research Data Repository
collection Online Access
description The principal challenge for the use of boronic acids (BA) as glucose sensors is their lack of specificity for glucose. We examined the selectivity of and insulin release from two boronic acids- (2-formyl-3-thienylboronic acid (FTBA) and 4-formylphenylboronic acid (FPBA)) conjugated chitosan scaffolds to glucose and fructose. Adsorption of glucose to BA: chitosan conjugates was dose-dependent up to 1:1 at 35 and 42% for FPBA and FTBA respectively but the FTBA conjugates adsorbed more glucose and fructose at respective FPBA ratios. The affinity of both BA conjugates to glucose decreased with increase in BA ratio. On the other hand, the affinity of both BA conjugates for fructose decreased from ratio 1:1 to 2:1 then rose again at 3:1. Insulin release from FPBA nanoparticles (FPBAINP) and FTBA nanoparticles (FTBAINP) were both concentration-dependent within glyceamically relevant values (1–3 mg/ml glucose and 0.002 mg/ml fructose). Furthermore, the total amounts of insulin released from FPBAINP in both the media were higher than from FTBAINP. Both FPBAINP and FTBAINP have the potential for development as a glucose-selective insulin delivery system in physiological settings.
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spelling nottingham-430032025-09-09T13:39:57Z https://eprints.nottingham.ac.uk/43003/ Multiboronic acid-conjugated chitosan scaffolds with glucose selectivity to insulin release Siddiqui, Nabil A. Billa, Nashiru Roberts, Clive J. The principal challenge for the use of boronic acids (BA) as glucose sensors is their lack of specificity for glucose. We examined the selectivity of and insulin release from two boronic acids- (2-formyl-3-thienylboronic acid (FTBA) and 4-formylphenylboronic acid (FPBA)) conjugated chitosan scaffolds to glucose and fructose. Adsorption of glucose to BA: chitosan conjugates was dose-dependent up to 1:1 at 35 and 42% for FPBA and FTBA respectively but the FTBA conjugates adsorbed more glucose and fructose at respective FPBA ratios. The affinity of both BA conjugates to glucose decreased with increase in BA ratio. On the other hand, the affinity of both BA conjugates for fructose decreased from ratio 1:1 to 2:1 then rose again at 3:1. Insulin release from FPBA nanoparticles (FPBAINP) and FTBA nanoparticles (FTBAINP) were both concentration-dependent within glyceamically relevant values (1–3 mg/ml glucose and 0.002 mg/ml fructose). Furthermore, the total amounts of insulin released from FPBAINP in both the media were higher than from FTBAINP. Both FPBAINP and FTBAINP have the potential for development as a glucose-selective insulin delivery system in physiological settings. Taylor & Francis 2017-03-09 Article PeerReviewed Siddiqui, Nabil A., Billa, Nashiru and Roberts, Clive J. (2017) Multiboronic acid-conjugated chitosan scaffolds with glucose selectivity to insulin release. Journal of Biomaterials Science, Polymer Edition, 28 (8). pp. 781-793. ISSN 1568-5624 Boronic acid chitosan glucose insulin nanoparticles http://www.tandfonline.com/doi/full/10.1080/09205063.2017.1301774 doi:10.1080/09205063.2017.1301774 doi:10.1080/09205063.2017.1301774
spellingShingle Boronic acid
chitosan
glucose
insulin
nanoparticles
Siddiqui, Nabil A.
Billa, Nashiru
Roberts, Clive J.
Multiboronic acid-conjugated chitosan scaffolds with glucose selectivity to insulin release
title Multiboronic acid-conjugated chitosan scaffolds with glucose selectivity to insulin release
title_full Multiboronic acid-conjugated chitosan scaffolds with glucose selectivity to insulin release
title_fullStr Multiboronic acid-conjugated chitosan scaffolds with glucose selectivity to insulin release
title_full_unstemmed Multiboronic acid-conjugated chitosan scaffolds with glucose selectivity to insulin release
title_short Multiboronic acid-conjugated chitosan scaffolds with glucose selectivity to insulin release
title_sort multiboronic acid-conjugated chitosan scaffolds with glucose selectivity to insulin release
topic Boronic acid
chitosan
glucose
insulin
nanoparticles
url https://eprints.nottingham.ac.uk/43003/
https://eprints.nottingham.ac.uk/43003/
https://eprints.nottingham.ac.uk/43003/