Identification of factors required for m6A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI
N6-adenosine methylation (m6A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m6A writer p...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
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Wiley
2017
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| Online Access: | https://eprints.nottingham.ac.uk/42964/ |
| _version_ | 1848796612925587456 |
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| author | Růžička, Kamil Zhang, Mi Campilho, Ana Bodi, Zsuzsanna Kashif, Muhammad Saleh, Mária Eeckhout, Dominique El-Showk, Sedeer Li, Hongying Zhong, Silin Jaeger, Geert De Mongan, Nigel P. Hejátko, Jan Helariutta, Ykä Fray, Rupert G. |
| author_facet | Růžička, Kamil Zhang, Mi Campilho, Ana Bodi, Zsuzsanna Kashif, Muhammad Saleh, Mária Eeckhout, Dominique El-Showk, Sedeer Li, Hongying Zhong, Silin Jaeger, Geert De Mongan, Nigel P. Hejátko, Jan Helariutta, Ykä Fray, Rupert G. |
| author_sort | Růžička, Kamil |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | N6-adenosine methylation (m6A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood.
Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m6A writer proteins in Arabidopsis thaliana.
The components required for m6A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m6A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m6A methylation plays a role in developmental decisions during pattern formation.
The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m6A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences. |
| first_indexed | 2025-11-14T19:50:45Z |
| format | Article |
| id | nottingham-42964 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:50:45Z |
| publishDate | 2017 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-429642020-05-04T18:45:55Z https://eprints.nottingham.ac.uk/42964/ Identification of factors required for m6A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI Růžička, Kamil Zhang, Mi Campilho, Ana Bodi, Zsuzsanna Kashif, Muhammad Saleh, Mária Eeckhout, Dominique El-Showk, Sedeer Li, Hongying Zhong, Silin Jaeger, Geert De Mongan, Nigel P. Hejátko, Jan Helariutta, Ykä Fray, Rupert G. N6-adenosine methylation (m6A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m6A writer proteins in Arabidopsis thaliana. The components required for m6A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m6A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m6A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m6A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences. Wiley 2017-05-15 Article PeerReviewed Růžička, Kamil, Zhang, Mi, Campilho, Ana, Bodi, Zsuzsanna, Kashif, Muhammad, Saleh, Mária, Eeckhout, Dominique, El-Showk, Sedeer, Li, Hongying, Zhong, Silin, Jaeger, Geert De, Mongan, Nigel P., Hejátko, Jan, Helariutta, Ykä and Fray, Rupert G. (2017) Identification of factors required for m6A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI. New Phytologist . ISSN 1469-8137 Arabidopsis HAKAI mRNA methylation N6-adenosine methylation (m6A) Protoxylem VIRILIZER https://doi.org/10.1111/nph.14586 doi:10.1111/nph.14586 doi:10.1111/nph.14586 |
| spellingShingle | Arabidopsis HAKAI mRNA methylation N6-adenosine methylation (m6A) Protoxylem VIRILIZER Růžička, Kamil Zhang, Mi Campilho, Ana Bodi, Zsuzsanna Kashif, Muhammad Saleh, Mária Eeckhout, Dominique El-Showk, Sedeer Li, Hongying Zhong, Silin Jaeger, Geert De Mongan, Nigel P. Hejátko, Jan Helariutta, Ykä Fray, Rupert G. Identification of factors required for m6A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI |
| title | Identification of factors required for m6A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI |
| title_full | Identification of factors required for m6A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI |
| title_fullStr | Identification of factors required for m6A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI |
| title_full_unstemmed | Identification of factors required for m6A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI |
| title_short | Identification of factors required for m6A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI |
| title_sort | identification of factors required for m6a mrna methylation in arabidopsis reveals a role for the conserved e3 ubiquitin ligase hakai |
| topic | Arabidopsis HAKAI mRNA methylation N6-adenosine methylation (m6A) Protoxylem VIRILIZER |
| url | https://eprints.nottingham.ac.uk/42964/ https://eprints.nottingham.ac.uk/42964/ https://eprints.nottingham.ac.uk/42964/ |