Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology
A miniaturized, high-throughput assay was optimized to screen polymer-drug solid dispersions using a 2-D Ink-jet printer. By simply printing nanoliter amounts of polymer and drug solutions onto an inert surface, drug:polymer micro-dots of tunable composition were produced in an easily-addressable mi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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American Chemical Society
2017
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| Online Access: | https://eprints.nottingham.ac.uk/42908/ |
| _version_ | 1848796599112695808 |
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| author | Taresco, Vincenzo Louzao, Iria Scurr, David J. Turpin, Eleanor R. Laughton, Charles A. Alexander, Cameron Burley, Jonathan C. Garnett, Martin |
| author_facet | Taresco, Vincenzo Louzao, Iria Scurr, David J. Turpin, Eleanor R. Laughton, Charles A. Alexander, Cameron Burley, Jonathan C. Garnett, Martin |
| author_sort | Taresco, Vincenzo |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | A miniaturized, high-throughput assay was optimized to screen polymer-drug solid dispersions using a 2-D Ink-jet printer. By simply printing nanoliter amounts of polymer and drug solutions onto an inert surface, drug:polymer micro-dots of tunable composition were produced in an easily-addressable micro-array format. The amount of material printed for each dried spot ranged from 25 ng to 650 ng. These arrays were used to assess the stability of drug:polymer dispersions with respect to recrystallization, using polarized light microscopy. One array with a panel of 6 drugs formulated at different ratios with Poly (vinylpyrrolidone-vinyl acetate) copolymer (PVPVA) was developed to estimate a possible bulk (gram-scale) approximation threshold from the final printed nano amount of formulation. Another array was printed at a fixed final amount of material to establish a literature comparison of one drug formulated with different commercial polymers for validation. This new approach may offer significant efficiency in pharmaceutical formulation screening, with each experiment in the nano-micro-array format requiring from 3 up to 6 orders of magnitude lower amounts of sample than conventional screening methods. |
| first_indexed | 2025-11-14T19:50:32Z |
| format | Article |
| id | nottingham-42908 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:50:32Z |
| publishDate | 2017 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-429082020-05-04T18:45:37Z https://eprints.nottingham.ac.uk/42908/ Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology Taresco, Vincenzo Louzao, Iria Scurr, David J. Turpin, Eleanor R. Laughton, Charles A. Alexander, Cameron Burley, Jonathan C. Garnett, Martin A miniaturized, high-throughput assay was optimized to screen polymer-drug solid dispersions using a 2-D Ink-jet printer. By simply printing nanoliter amounts of polymer and drug solutions onto an inert surface, drug:polymer micro-dots of tunable composition were produced in an easily-addressable micro-array format. The amount of material printed for each dried spot ranged from 25 ng to 650 ng. These arrays were used to assess the stability of drug:polymer dispersions with respect to recrystallization, using polarized light microscopy. One array with a panel of 6 drugs formulated at different ratios with Poly (vinylpyrrolidone-vinyl acetate) copolymer (PVPVA) was developed to estimate a possible bulk (gram-scale) approximation threshold from the final printed nano amount of formulation. Another array was printed at a fixed final amount of material to establish a literature comparison of one drug formulated with different commercial polymers for validation. This new approach may offer significant efficiency in pharmaceutical formulation screening, with each experiment in the nano-micro-array format requiring from 3 up to 6 orders of magnitude lower amounts of sample than conventional screening methods. American Chemical Society 2017-05-14 Article PeerReviewed Taresco, Vincenzo, Louzao, Iria, Scurr, David J., Turpin, Eleanor R., Laughton, Charles A., Alexander, Cameron, Burley, Jonathan C. and Garnett, Martin (2017) Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology. Molecular Pharmaceutics, 14 (6). pp. 2079-2087. ISSN 1543-8384 High throughput array Ink-jet printing Amorphous dispersion Recrystallisation Polarizing microscopy http://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.7b00182 doi:10.1021/acs.molpharmaceut.7b00182 doi:10.1021/acs.molpharmaceut.7b00182 |
| spellingShingle | High throughput array Ink-jet printing Amorphous dispersion Recrystallisation Polarizing microscopy Taresco, Vincenzo Louzao, Iria Scurr, David J. Turpin, Eleanor R. Laughton, Charles A. Alexander, Cameron Burley, Jonathan C. Garnett, Martin Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology |
| title | Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology |
| title_full | Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology |
| title_fullStr | Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology |
| title_full_unstemmed | Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology |
| title_short | Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology |
| title_sort | rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology |
| topic | High throughput array Ink-jet printing Amorphous dispersion Recrystallisation Polarizing microscopy |
| url | https://eprints.nottingham.ac.uk/42908/ https://eprints.nottingham.ac.uk/42908/ https://eprints.nottingham.ac.uk/42908/ |