RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator
Homologous recombination plays a central role in the repair of double-strand DNA breaks, the restart of stalled replication forks and the generation of genetic diversity. Regulation of recombination is essential since defects can lead to genome instability and chromosomal rearrangements. Strand exch...
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| Format: | Article |
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Elsevier
2017
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| Online Access: | https://eprints.nottingham.ac.uk/42849/ |
| _version_ | 1848796584087650304 |
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| author | Wardell, Kayleigh Haldenby, Sam Jones, Nathan Liddell, Susan Ngo, Greg H.P. Allers, Thorsten |
| author_facet | Wardell, Kayleigh Haldenby, Sam Jones, Nathan Liddell, Susan Ngo, Greg H.P. Allers, Thorsten |
| author_sort | Wardell, Kayleigh |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Homologous recombination plays a central role in the repair of double-strand DNA breaks, the restart of stalled replication forks and the generation of genetic diversity. Regulation of recombination is essential since defects can lead to genome instability and chromosomal rearrangements. Strand exchange is a key step of recombination – it is catalysed by RecA in bacteria, Rad51/Dmc1 in eukaryotes and RadA in archaea. RadB, a paralogue of RadA, is present in many archaeal species. RadB has previously been proposed to function as a recombination mediator, assisting in RadA-mediated strand exchange. In this study, we use the archaeon Haloferax volcanii to provide evidence to support this hypothesis. We show that RadB is required for efficient recombination and survival following treatment with DNA-damaging agents, and we identify two point mutations in radA that suppress the ΔradB phenotype. Analysis of these point mutations leads us to propose that the role of RadB is to act as a recombination mediator, which it does by inducing a conformational change in RadA and thereby promoting its polymerisation on DNA. |
| first_indexed | 2025-11-14T19:50:18Z |
| format | Article |
| id | nottingham-42849 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:50:18Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-428492020-05-04T19:56:45Z https://eprints.nottingham.ac.uk/42849/ RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator Wardell, Kayleigh Haldenby, Sam Jones, Nathan Liddell, Susan Ngo, Greg H.P. Allers, Thorsten Homologous recombination plays a central role in the repair of double-strand DNA breaks, the restart of stalled replication forks and the generation of genetic diversity. Regulation of recombination is essential since defects can lead to genome instability and chromosomal rearrangements. Strand exchange is a key step of recombination – it is catalysed by RecA in bacteria, Rad51/Dmc1 in eukaryotes and RadA in archaea. RadB, a paralogue of RadA, is present in many archaeal species. RadB has previously been proposed to function as a recombination mediator, assisting in RadA-mediated strand exchange. In this study, we use the archaeon Haloferax volcanii to provide evidence to support this hypothesis. We show that RadB is required for efficient recombination and survival following treatment with DNA-damaging agents, and we identify two point mutations in radA that suppress the ΔradB phenotype. Analysis of these point mutations leads us to propose that the role of RadB is to act as a recombination mediator, which it does by inducing a conformational change in RadA and thereby promoting its polymerisation on DNA. Elsevier 2017-07 Article PeerReviewed Wardell, Kayleigh, Haldenby, Sam, Jones, Nathan, Liddell, Susan, Ngo, Greg H.P. and Allers, Thorsten (2017) RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator. DNA Repair, 55 . pp. 7-16. ISSN 1568-7856 Homologous recombination; Archaea; RecA-family recombinase; Strand exchange; Recombination mediator http://www.sciencedirect.com/science/article/pii/S1568786415301117 doi:10.1016/j.dnarep.2017.04.005 doi:10.1016/j.dnarep.2017.04.005 |
| spellingShingle | Homologous recombination; Archaea; RecA-family recombinase; Strand exchange; Recombination mediator Wardell, Kayleigh Haldenby, Sam Jones, Nathan Liddell, Susan Ngo, Greg H.P. Allers, Thorsten RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator |
| title | RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator |
| title_full | RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator |
| title_fullStr | RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator |
| title_full_unstemmed | RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator |
| title_short | RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator |
| title_sort | radb acts in homologous recombination in the archaeon haloferax volcanii, consistent with a role as recombination mediator |
| topic | Homologous recombination; Archaea; RecA-family recombinase; Strand exchange; Recombination mediator |
| url | https://eprints.nottingham.ac.uk/42849/ https://eprints.nottingham.ac.uk/42849/ https://eprints.nottingham.ac.uk/42849/ |