Hes3 expression in the adult mouse brain is regulated during demyelination and remyelination
Hes3 is a component of the STAT3-Ser/Hes3 Signaling Axis controlling the growth and survival of neural stem cells and other plastic cells. Pharmacological activation of this pathway promotes neuronal rescue and behavioral recovery in models of ischemic stroke and Parkinson's disease. Here we pr...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
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Elsevier
2016
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| Online Access: | https://eprints.nottingham.ac.uk/42833/ |
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| author | Toutouna, Louiza Nikolakopoulou, Polyxeni Poser, Steven W. Masjkur, Jimmy Arps-Forker, Carina Troullinaki, Maria Grossklaus, Sylvia Bosak, Viktoria Friedrich, Ulrike Ziemssen, Tjalf Bornstein, Stefan R. Chavakis, Triantafyllos Androutsellis-Theotokis, Andreas |
| author_facet | Toutouna, Louiza Nikolakopoulou, Polyxeni Poser, Steven W. Masjkur, Jimmy Arps-Forker, Carina Troullinaki, Maria Grossklaus, Sylvia Bosak, Viktoria Friedrich, Ulrike Ziemssen, Tjalf Bornstein, Stefan R. Chavakis, Triantafyllos Androutsellis-Theotokis, Andreas |
| author_sort | Toutouna, Louiza |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Hes3 is a component of the STAT3-Ser/Hes3 Signaling Axis controlling the growth and survival of neural stem cells and other plastic cells. Pharmacological activation of this pathway promotes neuronal rescue and behavioral recovery in models of ischemic stroke and Parkinson's disease. Here we provide initial observations implicating Hes3 in the cuprizone model of demyelination and remyelination. We focus on the subpial motor cortex of mice because we detected high Hes3 expression. This area is of interest as it is impacted both in human demyelinating diseases and in the cuprizone model. We report that Hes3 expression is reduced at peak demyelination and is partially restored within 1 week after cuprizone withdrawal. This raises the possibility of Hes3 involvement in demyelination/remyelination that may warrant additional research. Supporting a possible role of Hes3 in the maintenance of oligodendrocyte markers, a Hes3 null mouse strain shows lower levels of myelin basic protein in undamaged adult mice, compared to wild-type controls. We also present a novel method for culturing the established oligodendrocyte progenitor cell line oli-neu in a manner that maintains Hes3 expression as well as its self-renewal and differentiation potential, offering an experimental tool to study Hes3. Based upon this approach, we identify a Janus kinase inhibitor and dbcAMP as powerful inducers of Hes3 gene expression. We provide a new biomarker and cell culture method that may be of interest in demyelination/remyelination research. |
| first_indexed | 2025-11-14T19:50:14Z |
| format | Article |
| id | nottingham-42833 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:50:14Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-428332020-05-04T17:54:15Z https://eprints.nottingham.ac.uk/42833/ Hes3 expression in the adult mouse brain is regulated during demyelination and remyelination Toutouna, Louiza Nikolakopoulou, Polyxeni Poser, Steven W. Masjkur, Jimmy Arps-Forker, Carina Troullinaki, Maria Grossklaus, Sylvia Bosak, Viktoria Friedrich, Ulrike Ziemssen, Tjalf Bornstein, Stefan R. Chavakis, Triantafyllos Androutsellis-Theotokis, Andreas Hes3 is a component of the STAT3-Ser/Hes3 Signaling Axis controlling the growth and survival of neural stem cells and other plastic cells. Pharmacological activation of this pathway promotes neuronal rescue and behavioral recovery in models of ischemic stroke and Parkinson's disease. Here we provide initial observations implicating Hes3 in the cuprizone model of demyelination and remyelination. We focus on the subpial motor cortex of mice because we detected high Hes3 expression. This area is of interest as it is impacted both in human demyelinating diseases and in the cuprizone model. We report that Hes3 expression is reduced at peak demyelination and is partially restored within 1 week after cuprizone withdrawal. This raises the possibility of Hes3 involvement in demyelination/remyelination that may warrant additional research. Supporting a possible role of Hes3 in the maintenance of oligodendrocyte markers, a Hes3 null mouse strain shows lower levels of myelin basic protein in undamaged adult mice, compared to wild-type controls. We also present a novel method for culturing the established oligodendrocyte progenitor cell line oli-neu in a manner that maintains Hes3 expression as well as its self-renewal and differentiation potential, offering an experimental tool to study Hes3. Based upon this approach, we identify a Janus kinase inhibitor and dbcAMP as powerful inducers of Hes3 gene expression. We provide a new biomarker and cell culture method that may be of interest in demyelination/remyelination research. Elsevier 2016-07-01 Article PeerReviewed Toutouna, Louiza, Nikolakopoulou, Polyxeni, Poser, Steven W., Masjkur, Jimmy, Arps-Forker, Carina, Troullinaki, Maria, Grossklaus, Sylvia, Bosak, Viktoria, Friedrich, Ulrike, Ziemssen, Tjalf, Bornstein, Stefan R., Chavakis, Triantafyllos and Androutsellis-Theotokis, Andreas (2016) Hes3 expression in the adult mouse brain is regulated during demyelination and remyelination. Brain Research, 1642 . pp. 124-130. ISSN 1872-6240 Hes3; Myelin; Oligodendrocyte precursor; Brain; Regeneration http://www.sciencedirect.com/science/article/pii/S0006899316301536 doi:10.1016/j.brainres.2016.03.014 doi:10.1016/j.brainres.2016.03.014 |
| spellingShingle | Hes3; Myelin; Oligodendrocyte precursor; Brain; Regeneration Toutouna, Louiza Nikolakopoulou, Polyxeni Poser, Steven W. Masjkur, Jimmy Arps-Forker, Carina Troullinaki, Maria Grossklaus, Sylvia Bosak, Viktoria Friedrich, Ulrike Ziemssen, Tjalf Bornstein, Stefan R. Chavakis, Triantafyllos Androutsellis-Theotokis, Andreas Hes3 expression in the adult mouse brain is regulated during demyelination and remyelination |
| title | Hes3 expression in the adult mouse brain is regulated during demyelination and remyelination |
| title_full | Hes3 expression in the adult mouse brain is regulated during demyelination and remyelination |
| title_fullStr | Hes3 expression in the adult mouse brain is regulated during demyelination and remyelination |
| title_full_unstemmed | Hes3 expression in the adult mouse brain is regulated during demyelination and remyelination |
| title_short | Hes3 expression in the adult mouse brain is regulated during demyelination and remyelination |
| title_sort | hes3 expression in the adult mouse brain is regulated during demyelination and remyelination |
| topic | Hes3; Myelin; Oligodendrocyte precursor; Brain; Regeneration |
| url | https://eprints.nottingham.ac.uk/42833/ https://eprints.nottingham.ac.uk/42833/ https://eprints.nottingham.ac.uk/42833/ |