Expression of the transcription factor Hes3 in the mouse and human ocular surface, and in pterygium
Purpose: In this work we examined the presence of the neural stem cell biomarker Hairy and Enhancer of Split 3 (Hes3) in the anterior eye segment and in the aberrant growth condition of the conjunctiva pterygium. Further, we studied the response of Hes3 to irradiation. Materials and methods: Adul...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Taylor & Francis
2014
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/42825/ |
| _version_ | 1848796577570750464 |
|---|---|
| author | Economopoulou, Matina Masjkur, Jimmy Raiskup, Frederik Ebermann, Doreen Saha, Sarama Karl, Mike O. Funk, Richard Jaszai, Jozsef Chavakis, Triantafyllos Ehrhart-Bornstein, Monika Pillunat, Lutz E. Kunz-Schughart, Leoni Kurth, Ina Dubrovska, Anna Androutsellis-Theotokis, Andreas |
| author_facet | Economopoulou, Matina Masjkur, Jimmy Raiskup, Frederik Ebermann, Doreen Saha, Sarama Karl, Mike O. Funk, Richard Jaszai, Jozsef Chavakis, Triantafyllos Ehrhart-Bornstein, Monika Pillunat, Lutz E. Kunz-Schughart, Leoni Kurth, Ina Dubrovska, Anna Androutsellis-Theotokis, Andreas |
| author_sort | Economopoulou, Matina |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Purpose: In this work we examined the presence of the neural stem cell biomarker Hairy and Enhancer of Split 3 (Hes3) in the anterior eye segment and in the aberrant growth condition of the conjunctiva pterygium. Further, we studied the response of Hes3 to irradiation.
Materials and methods: Adult mouse and human corneoscleral junction and conjunctiva, as well as human pterygium were prepared for immunohistochemical detection of Hes3 and other markers. Total body irradiation was used to study the changes in the pattern of Hes3 expression.
Results: The adult rodent and human eye as well as pterygium, contain a population of cells expressing Hes3. In the human eye, Hes3-expressing (Hes3+) cells are found predominantly in the subconjunctival space spanning over the limbus where they physically associate with blood vessels. The cytoarchitecture of Hes3 + cells is similar to those previously observed in the adult central nervous system. Furthermore, irradiation reduces the number of Hes3 + cells in the subconjunctival space. In contrast, irradiation strongly promotes the nuclear localization of Hes3 in the ciliary body epithelium.
Conclusions: Our results suggest that a recently identified signal transduction pathway that regulates neural stem cells and glioblastoma cancer stem cells also operates in the ocular surface, ciliary body, and in pterygium. |
| first_indexed | 2025-11-14T19:50:12Z |
| format | Article |
| id | nottingham-42825 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:50:12Z |
| publishDate | 2014 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-428252020-05-04T16:44:19Z https://eprints.nottingham.ac.uk/42825/ Expression of the transcription factor Hes3 in the mouse and human ocular surface, and in pterygium Economopoulou, Matina Masjkur, Jimmy Raiskup, Frederik Ebermann, Doreen Saha, Sarama Karl, Mike O. Funk, Richard Jaszai, Jozsef Chavakis, Triantafyllos Ehrhart-Bornstein, Monika Pillunat, Lutz E. Kunz-Schughart, Leoni Kurth, Ina Dubrovska, Anna Androutsellis-Theotokis, Andreas Purpose: In this work we examined the presence of the neural stem cell biomarker Hairy and Enhancer of Split 3 (Hes3) in the anterior eye segment and in the aberrant growth condition of the conjunctiva pterygium. Further, we studied the response of Hes3 to irradiation. Materials and methods: Adult mouse and human corneoscleral junction and conjunctiva, as well as human pterygium were prepared for immunohistochemical detection of Hes3 and other markers. Total body irradiation was used to study the changes in the pattern of Hes3 expression. Results: The adult rodent and human eye as well as pterygium, contain a population of cells expressing Hes3. In the human eye, Hes3-expressing (Hes3+) cells are found predominantly in the subconjunctival space spanning over the limbus where they physically associate with blood vessels. The cytoarchitecture of Hes3 + cells is similar to those previously observed in the adult central nervous system. Furthermore, irradiation reduces the number of Hes3 + cells in the subconjunctival space. In contrast, irradiation strongly promotes the nuclear localization of Hes3 in the ciliary body epithelium. Conclusions: Our results suggest that a recently identified signal transduction pathway that regulates neural stem cells and glioblastoma cancer stem cells also operates in the ocular surface, ciliary body, and in pterygium. Taylor & Francis 2014-03-25 Article PeerReviewed Economopoulou, Matina, Masjkur, Jimmy, Raiskup, Frederik, Ebermann, Doreen, Saha, Sarama, Karl, Mike O., Funk, Richard, Jaszai, Jozsef, Chavakis, Triantafyllos, Ehrhart-Bornstein, Monika, Pillunat, Lutz E., Kunz-Schughart, Leoni, Kurth, Ina, Dubrovska, Anna and Androutsellis-Theotokis, Andreas (2014) Expression of the transcription factor Hes3 in the mouse and human ocular surface, and in pterygium. International Journal of Radiation Biology, 90 (8). pp. 700-709. ISSN 1362-3095 Hes3 neural stem cells eye pterygium conjunctiva irradiation http://www.tandfonline.com/doi/full/10.3109/09553002.2014.892228 doi:10.3109/09553002.2014.892228 doi:10.3109/09553002.2014.892228 |
| spellingShingle | Hes3 neural stem cells eye pterygium conjunctiva irradiation Economopoulou, Matina Masjkur, Jimmy Raiskup, Frederik Ebermann, Doreen Saha, Sarama Karl, Mike O. Funk, Richard Jaszai, Jozsef Chavakis, Triantafyllos Ehrhart-Bornstein, Monika Pillunat, Lutz E. Kunz-Schughart, Leoni Kurth, Ina Dubrovska, Anna Androutsellis-Theotokis, Andreas Expression of the transcription factor Hes3 in the mouse and human ocular surface, and in pterygium |
| title | Expression of the transcription factor Hes3 in the mouse and human ocular surface, and in pterygium |
| title_full | Expression of the transcription factor Hes3 in the mouse and human ocular surface, and in pterygium |
| title_fullStr | Expression of the transcription factor Hes3 in the mouse and human ocular surface, and in pterygium |
| title_full_unstemmed | Expression of the transcription factor Hes3 in the mouse and human ocular surface, and in pterygium |
| title_short | Expression of the transcription factor Hes3 in the mouse and human ocular surface, and in pterygium |
| title_sort | expression of the transcription factor hes3 in the mouse and human ocular surface, and in pterygium |
| topic | Hes3 neural stem cells eye pterygium conjunctiva irradiation |
| url | https://eprints.nottingham.ac.uk/42825/ https://eprints.nottingham.ac.uk/42825/ https://eprints.nottingham.ac.uk/42825/ |