Immunostaining for DNA modifications: computational analysis of confocal images

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Immunostaining for DNA modifications: computational analysis of confocal images

For several decades, 5-methylcytosine (5mC) has been thought to be the only DNA modification with a functional significance in metazoans. The discovery of enzymatic oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) as well as detection of N6-met...

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Bibliographic Details
Main Authors:	Ramsawhook, Ashley, Lewis, Lara C., Eleftheriou, Maria, Abakir, Abdulkadir, Durczak, Paulina M., Markus, Robert, Rajini, Seema, Hannan, Nicholas R.F., Coyle, Beth, Ruzov, Alexey
Format:	Article
Published:	Journal of Visualized Experiments 2017
Subjects:	Epigenetics DNA oxi-mCs N6-methyladenine Immunohistochemistry Zen Confocal Microscopy Signal Quantitation Spatial Distribution
Online Access:	https://eprints.nottingham.ac.uk/42816/

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