Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan
Chitosan, a soluble polycationic derivative of insoluble chitin, has been widely considered for use in the food, cosmetic and pharmaceutical industries. Commercial (“C”) and in-house laboratory (“L”) prepared chitosan samples extracted from crustaceous shells with different molecular weight and degr...
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| Format: | Article |
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Elsevier
2015
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| Online Access: | https://eprints.nottingham.ac.uk/42762/ |
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| author | Almutairi, Fahad M. Erten, Tayyibe Adams, Gary G. Hayes, Maria McLoughlin, Pádraig Kök, M. Şamil Mackie, Alan R. Rowe, Arthur J. Harding, Stephen E. |
| author_facet | Almutairi, Fahad M. Erten, Tayyibe Adams, Gary G. Hayes, Maria McLoughlin, Pádraig Kök, M. Şamil Mackie, Alan R. Rowe, Arthur J. Harding, Stephen E. |
| author_sort | Almutairi, Fahad M. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Chitosan, a soluble polycationic derivative of insoluble chitin, has been widely considered for use in the food, cosmetic and pharmaceutical industries. Commercial (“C”) and in-house laboratory (“L”) prepared chitosan samples extracted from crustaceous shells with different molecular weight and degrees of acetylation (25% and 15%) were compared with regards to (i) weight–average molecular weight (Mw); (ii) sedimentation coefficient (so20,w) distribution, and (iii) intrinsic viscosity ([η]). These parameters were estimated using a combination of analytical ultracentrifugation (AUC), size exclusion chromatography coupled to multi-angle laser light scattering (SEC–MALS) and differential pressure viscometry. Polydisperse distributions were seen from sedimentation coefficient distributions and elution profiles from SEC–MALS. Mw values obtained for each sample by sedimentation equilibrium measurements were in excellent agreement with those obtained from SEC–MALS. Mark–Houwink–Kuhn–Sakurada (MHKS) and Wales van Holde analyses of the data all suggest a semi-flexible conformation.
The principle of co-sedimentation was then used to monitor the interactions of the two different molecular weights of L chitosans with two polyanions, DNA and xanthan (another double helical high molecular weight molecule). Interactions were clearly observed and then quantified from the changes in the sedimentation coefficient distribution of the mixture compared to unmixed controls using sedimentation velocity. The interactions appeared to show a strong dependence on molecular weight. The relevance of this for DNA condensation applications is indicated. |
| first_indexed | 2025-11-14T19:49:56Z |
| format | Article |
| id | nottingham-42762 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:49:56Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-427622020-05-04T17:08:18Z https://eprints.nottingham.ac.uk/42762/ Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan Almutairi, Fahad M. Erten, Tayyibe Adams, Gary G. Hayes, Maria McLoughlin, Pádraig Kök, M. Şamil Mackie, Alan R. Rowe, Arthur J. Harding, Stephen E. Chitosan, a soluble polycationic derivative of insoluble chitin, has been widely considered for use in the food, cosmetic and pharmaceutical industries. Commercial (“C”) and in-house laboratory (“L”) prepared chitosan samples extracted from crustaceous shells with different molecular weight and degrees of acetylation (25% and 15%) were compared with regards to (i) weight–average molecular weight (Mw); (ii) sedimentation coefficient (so20,w) distribution, and (iii) intrinsic viscosity ([η]). These parameters were estimated using a combination of analytical ultracentrifugation (AUC), size exclusion chromatography coupled to multi-angle laser light scattering (SEC–MALS) and differential pressure viscometry. Polydisperse distributions were seen from sedimentation coefficient distributions and elution profiles from SEC–MALS. Mw values obtained for each sample by sedimentation equilibrium measurements were in excellent agreement with those obtained from SEC–MALS. Mark–Houwink–Kuhn–Sakurada (MHKS) and Wales van Holde analyses of the data all suggest a semi-flexible conformation. The principle of co-sedimentation was then used to monitor the interactions of the two different molecular weights of L chitosans with two polyanions, DNA and xanthan (another double helical high molecular weight molecule). Interactions were clearly observed and then quantified from the changes in the sedimentation coefficient distribution of the mixture compared to unmixed controls using sedimentation velocity. The interactions appeared to show a strong dependence on molecular weight. The relevance of this for DNA condensation applications is indicated. Elsevier 2015-05-20 Article PeerReviewed Almutairi, Fahad M., Erten, Tayyibe, Adams, Gary G., Hayes, Maria, McLoughlin, Pádraig, Kök, M. Şamil, Mackie, Alan R., Rowe, Arthur J. and Harding, Stephen E. (2015) Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan. Carbohydrate Polymers, 122 . pp. 359-366. ISSN 0144-8617 Sedimentation distribution; flexibility; co-sedimentation double helix multi-Gaussian fit http://www.sciencedirect.com/science/article/pii/S0144861714010030 doi:10.1016/j.carbpol.2014.09.090 doi:10.1016/j.carbpol.2014.09.090 |
| spellingShingle | Sedimentation distribution; flexibility; co-sedimentation double helix multi-Gaussian fit Almutairi, Fahad M. Erten, Tayyibe Adams, Gary G. Hayes, Maria McLoughlin, Pádraig Kök, M. Şamil Mackie, Alan R. Rowe, Arthur J. Harding, Stephen E. Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan |
| title | Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan |
| title_full | Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan |
| title_fullStr | Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan |
| title_full_unstemmed | Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan |
| title_short | Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan |
| title_sort | hydrodynamic characterisation of chitosan and its interaction with two polyanions: dna and xanthan |
| topic | Sedimentation distribution; flexibility; co-sedimentation double helix multi-Gaussian fit |
| url | https://eprints.nottingham.ac.uk/42762/ https://eprints.nottingham.ac.uk/42762/ https://eprints.nottingham.ac.uk/42762/ |