Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content

Introduction: The bacterial exopolysaccharide xanthan is a well-known hydrocolloid, with a high viscosity deriving from its large molecular weight and volume. Materials and methods: Four different xanthans deriving from Xanthomanas campestris -two with similar pyruvate and acetate contents and two...

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Main Authors: Erten, Tayyibe, Adams, Gary G., Foster, Timothy J., Harding, Stephen E.
Format: Article
Published: Elsevier 2014
Subjects:
Online Access:https://eprints.nottingham.ac.uk/42758/
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author Erten, Tayyibe
Adams, Gary G.
Foster, Timothy J.
Harding, Stephen E.
author_facet Erten, Tayyibe
Adams, Gary G.
Foster, Timothy J.
Harding, Stephen E.
author_sort Erten, Tayyibe
building Nottingham Research Data Repository
collection Online Access
description Introduction: The bacterial exopolysaccharide xanthan is a well-known hydrocolloid, with a high viscosity deriving from its large molecular weight and volume. Materials and methods: Four different xanthans deriving from Xanthomanas campestris -two with similar pyruvate and acetate contents and two with different contents e were characterized in dilute aqueous buffered solution (pH 7.0, ionic strength 0.3 M) using sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge, supplemented by dynamic light scattering. Results: This facilitated a comparison with regards heterogeneity (sedimentation coefficient distribution) and molecular weight, despite their large size and low critical overlap concentration. The xanthans were also compared with regard to reduced specific and intrinsic viscosity behaviour. The xanthans generally show strong similarity in properties with the exception of the lowest pyruvate xanthan, a finding which should be useful for future applications of these materials.
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spelling nottingham-427582020-05-04T16:58:38Z https://eprints.nottingham.ac.uk/42758/ Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content Erten, Tayyibe Adams, Gary G. Foster, Timothy J. Harding, Stephen E. Introduction: The bacterial exopolysaccharide xanthan is a well-known hydrocolloid, with a high viscosity deriving from its large molecular weight and volume. Materials and methods: Four different xanthans deriving from Xanthomanas campestris -two with similar pyruvate and acetate contents and two with different contents e were characterized in dilute aqueous buffered solution (pH 7.0, ionic strength 0.3 M) using sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge, supplemented by dynamic light scattering. Results: This facilitated a comparison with regards heterogeneity (sedimentation coefficient distribution) and molecular weight, despite their large size and low critical overlap concentration. The xanthans were also compared with regard to reduced specific and intrinsic viscosity behaviour. The xanthans generally show strong similarity in properties with the exception of the lowest pyruvate xanthan, a finding which should be useful for future applications of these materials. Elsevier 2014-12-15 Article PeerReviewed Erten, Tayyibe, Adams, Gary G., Foster, Timothy J. and Harding, Stephen E. (2014) Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content. Food Hydrocolloids, 42 . pp. 335-341. ISSN 0268-005X Xanthan ; Pyruvate ; Molecular weight ; Ultracentrifugation http://www.sciencedirect.com/science/article/pii/S0268005X14001428 doi:10.1016/j.foodhyd.2014.04.012 doi:10.1016/j.foodhyd.2014.04.012
spellingShingle Xanthan ; Pyruvate ; Molecular weight ; Ultracentrifugation
Erten, Tayyibe
Adams, Gary G.
Foster, Timothy J.
Harding, Stephen E.
Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content
title Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content
title_full Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content
title_fullStr Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content
title_full_unstemmed Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content
title_short Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content
title_sort comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content
topic Xanthan ; Pyruvate ; Molecular weight ; Ultracentrifugation
url https://eprints.nottingham.ac.uk/42758/
https://eprints.nottingham.ac.uk/42758/
https://eprints.nottingham.ac.uk/42758/