On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review
Background: Polysaccharides and their derivatives are increasingly being used by the food, cosmetic and pharmaceutical industries: physical properties like size and conformation are important contributors to their performance. Discussion: Here the use of hydrodynamic tools such as sedimentation vel...
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| Format: | Article |
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Elsevier
2014
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| Online Access: | https://eprints.nottingham.ac.uk/42756/ |
| _version_ | 1848796560062676992 |
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| author | Morris, Gordon A. Adams, Gary G. Harding, Stephen E. |
| author_facet | Morris, Gordon A. Adams, Gary G. Harding, Stephen E. |
| author_sort | Morris, Gordon A. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Background: Polysaccharides and their derivatives are increasingly being used by the food, cosmetic and pharmaceutical industries: physical properties like size and conformation are important contributors to their performance.
Discussion: Here the use of hydrodynamic tools such as sedimentation velocity, sedimentation equilibrium, size exclusion chromatography e multi-angle light scattering (SEC-MALS), and viscometry are considered highlighting some recent developments in methodology and the application of these to help better understand polysaccharide structureefunction relationships.
Conclusions: The size and shape of polysaccharides in solution can be estimated in a variety of ways. Molar masses and heterogeneities can be estimated to a good precision by sedimentation velocity, sedimentation equilibrium and SEC-MALS. An approximate idea of conformation and flexibility can be obtained from power-law coefficients and the Wales van Holde parameter. More sophisticated estimates can be obtained by combining methods together to yield the persistence length. |
| first_indexed | 2025-11-14T19:49:55Z |
| format | Article |
| id | nottingham-42756 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:49:55Z |
| publishDate | 2014 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-427562020-05-04T16:58:40Z https://eprints.nottingham.ac.uk/42756/ On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review Morris, Gordon A. Adams, Gary G. Harding, Stephen E. Background: Polysaccharides and their derivatives are increasingly being used by the food, cosmetic and pharmaceutical industries: physical properties like size and conformation are important contributors to their performance. Discussion: Here the use of hydrodynamic tools such as sedimentation velocity, sedimentation equilibrium, size exclusion chromatography e multi-angle light scattering (SEC-MALS), and viscometry are considered highlighting some recent developments in methodology and the application of these to help better understand polysaccharide structureefunction relationships. Conclusions: The size and shape of polysaccharides in solution can be estimated in a variety of ways. Molar masses and heterogeneities can be estimated to a good precision by sedimentation velocity, sedimentation equilibrium and SEC-MALS. An approximate idea of conformation and flexibility can be obtained from power-law coefficients and the Wales van Holde parameter. More sophisticated estimates can be obtained by combining methods together to yield the persistence length. Elsevier 2014-12-15 Article PeerReviewed Morris, Gordon A., Adams, Gary G. and Harding, Stephen E. (2014) On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review. Food Hydrocolloids, 42 . pp. 318-334. ISSN 0268-005X Polysaccharides ; Size ; Conformation ; Structure-function relationships http://www.sciencedirect.com/science/article/pii/S0268005X14001441 doi:10.1016/j.foodhyd.2014.04.014 doi:10.1016/j.foodhyd.2014.04.014 |
| spellingShingle | Polysaccharides ; Size ; Conformation ; Structure-function relationships Morris, Gordon A. Adams, Gary G. Harding, Stephen E. On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review |
| title | On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review |
| title_full | On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review |
| title_fullStr | On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review |
| title_full_unstemmed | On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review |
| title_short | On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review |
| title_sort | on hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review |
| topic | Polysaccharides ; Size ; Conformation ; Structure-function relationships |
| url | https://eprints.nottingham.ac.uk/42756/ https://eprints.nottingham.ac.uk/42756/ https://eprints.nottingham.ac.uk/42756/ |