Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation
According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidas...
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Elsevier
2014
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| Online Access: | https://eprints.nottingham.ac.uk/41765/ |
| _version_ | 1848796349716234240 |
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| author | Karlyshev, A.V. Thacker, G. Jones, M.A. Clements, M.O. Wren, Brendan W. |
| author_facet | Karlyshev, A.V. Thacker, G. Jones, M.A. Clements, M.O. Wren, Brendan W. |
| author_sort | Karlyshev, A.V. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection. |
| first_indexed | 2025-11-14T19:46:34Z |
| format | Article |
| id | nottingham-41765 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:46:34Z |
| publishDate | 2014 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-417652020-05-04T16:48:11Z https://eprints.nottingham.ac.uk/41765/ Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation Karlyshev, A.V. Thacker, G. Jones, M.A. Clements, M.O. Wren, Brendan W. According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection. Elsevier 2014-05-09 Article PeerReviewed Karlyshev, A.V., Thacker, G., Jones, M.A., Clements, M.O. and Wren, Brendan W. (2014) Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation. FEBS Open Bio, 4 (1). pp. 468-472. ISSN 2211-5463 Campylobacter Protease Colonisation Chicken http://onlinelibrary.wiley.com/doi/10.1016/j.fob.2014.04.012/epdf doi:10.1016/j.fob.2014.04.012 doi:10.1016/j.fob.2014.04.012 |
| spellingShingle | Campylobacter Protease Colonisation Chicken Karlyshev, A.V. Thacker, G. Jones, M.A. Clements, M.O. Wren, Brendan W. Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation |
| title | Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation |
| title_full | Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation |
| title_fullStr | Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation |
| title_full_unstemmed | Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation |
| title_short | Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation |
| title_sort | campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation |
| topic | Campylobacter Protease Colonisation Chicken |
| url | https://eprints.nottingham.ac.uk/41765/ https://eprints.nottingham.ac.uk/41765/ https://eprints.nottingham.ac.uk/41765/ |