Protein–like fully reversible tetramerisation and super-association of an aminocellulose

Unusual protein-like, partially reversible associative behaviour has recently been observed in solutions of the water soluble carbohydrates known as 6-deoxy-6-(v-aminoalkyl)aminocelluloses, which produce controllable self-assembling films for enzyme immobilisation and other biotechnological applicat...

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Main Authors: Nikolajski, Melanie, Adams, Gary G., Gillis, Richard B., Besong, David Tabot, Rowe, Arthur J., Heinze, Thomas, Harding, Stephen E.
Format: Article
Published: Nature Publishing Group 2014
Online Access:https://eprints.nottingham.ac.uk/41748/
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author Nikolajski, Melanie
Adams, Gary G.
Gillis, Richard B.
Besong, David Tabot
Rowe, Arthur J.
Heinze, Thomas
Harding, Stephen E.
author_facet Nikolajski, Melanie
Adams, Gary G.
Gillis, Richard B.
Besong, David Tabot
Rowe, Arthur J.
Heinze, Thomas
Harding, Stephen E.
author_sort Nikolajski, Melanie
building Nottingham Research Data Repository
collection Online Access
description Unusual protein-like, partially reversible associative behaviour has recently been observed in solutions of the water soluble carbohydrates known as 6-deoxy-6-(v-aminoalkyl)aminocelluloses, which produce controllable self-assembling films for enzyme immobilisation and other biotechnological applications. Now, for the first time, we have found a fully reversible self-association (tetramerisation) within this family of polysaccharides. Remarkably these carbohydrate tetramers are then seen to associate further in a regular way into supra-molecular complexes. Fully reversible oligomerisation has been hitherto completely unknown for carbohydrates and instead resembles in some respects the assembly of polypeptides and proteins like haemoglobin and its sickle cell mutation. Our traditional perceptions as to what might be considered ‘‘protein-like’’ and what might be considered as ‘‘carbohydrate-like’’ behaviour may need to be rendered more flexible, at least as far as interaction phenomena are concerned.
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spelling nottingham-417482020-05-04T16:41:19Z https://eprints.nottingham.ac.uk/41748/ Protein–like fully reversible tetramerisation and super-association of an aminocellulose Nikolajski, Melanie Adams, Gary G. Gillis, Richard B. Besong, David Tabot Rowe, Arthur J. Heinze, Thomas Harding, Stephen E. Unusual protein-like, partially reversible associative behaviour has recently been observed in solutions of the water soluble carbohydrates known as 6-deoxy-6-(v-aminoalkyl)aminocelluloses, which produce controllable self-assembling films for enzyme immobilisation and other biotechnological applications. Now, for the first time, we have found a fully reversible self-association (tetramerisation) within this family of polysaccharides. Remarkably these carbohydrate tetramers are then seen to associate further in a regular way into supra-molecular complexes. Fully reversible oligomerisation has been hitherto completely unknown for carbohydrates and instead resembles in some respects the assembly of polypeptides and proteins like haemoglobin and its sickle cell mutation. Our traditional perceptions as to what might be considered ‘‘protein-like’’ and what might be considered as ‘‘carbohydrate-like’’ behaviour may need to be rendered more flexible, at least as far as interaction phenomena are concerned. Nature Publishing Group 2014-01-24 Article PeerReviewed Nikolajski, Melanie, Adams, Gary G., Gillis, Richard B., Besong, David Tabot, Rowe, Arthur J., Heinze, Thomas and Harding, Stephen E. (2014) Protein–like fully reversible tetramerisation and super-association of an aminocellulose. Scientific Reports, 4 . 3861/1-3861/5. ISSN 2045-2322 https://doi.org/10.1038/srep03861 doi:10.1038/srep03861 doi:10.1038/srep03861
spellingShingle Nikolajski, Melanie
Adams, Gary G.
Gillis, Richard B.
Besong, David Tabot
Rowe, Arthur J.
Heinze, Thomas
Harding, Stephen E.
Protein–like fully reversible tetramerisation and super-association of an aminocellulose
title Protein–like fully reversible tetramerisation and super-association of an aminocellulose
title_full Protein–like fully reversible tetramerisation and super-association of an aminocellulose
title_fullStr Protein–like fully reversible tetramerisation and super-association of an aminocellulose
title_full_unstemmed Protein–like fully reversible tetramerisation and super-association of an aminocellulose
title_short Protein–like fully reversible tetramerisation and super-association of an aminocellulose
title_sort protein–like fully reversible tetramerisation and super-association of an aminocellulose
url https://eprints.nottingham.ac.uk/41748/
https://eprints.nottingham.ac.uk/41748/
https://eprints.nottingham.ac.uk/41748/