Subset-specific expression of toll-like receptors by bovine afferent lymph dendritic cells
Within the ruminant system, several possibilities exist to generate dendritic cells migrating out from the tissue into the regional draining lymph nodes as afferent lymph dendritic cells (ALDCs). Here, we analyzed toll-like receptor (TLR) 1–10 mRNA expression by using quantitative real-time PCR in h...
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| Format: | Article |
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Frontiers
2017
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| Online Access: | https://eprints.nottingham.ac.uk/41740/ |
| _version_ | 1848796342945579008 |
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| author | Werling, Dirk Hope, Jayne C. Siddiqui, Nazneen Widdison, Stephanie Russell, Chris Sopp, Paul Coffey, Tracey J. |
| author_facet | Werling, Dirk Hope, Jayne C. Siddiqui, Nazneen Widdison, Stephanie Russell, Chris Sopp, Paul Coffey, Tracey J. |
| author_sort | Werling, Dirk |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Within the ruminant system, several possibilities exist to generate dendritic cells migrating out from the tissue into the regional draining lymph nodes as afferent lymph dendritic cells (ALDCs). Here, we analyzed toll-like receptor (TLR) 1–10 mRNA expression by using quantitative real-time PCR in highly purified subsets of bovine ALDC. As TLR expression may be influenced by pathogens or vaccines and their adjuvant, it is necessary to understand what TLRs are expressed in a steady-state system to elucidate specific differences and to potentially optimize targeted vaccines. In this study, we have assessed the TLR expression profiles of the four main bovine ALDC subsets [cDC1 and cDC2 (subsets 2–4)]. We demonstrate differences in TLR expression between the four subsets that may reflect the ability of these cells to respond to different pathogens or to respond to adjuvants. |
| first_indexed | 2025-11-14T19:46:28Z |
| format | Article |
| id | nottingham-41740 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:46:28Z |
| publishDate | 2017 |
| publisher | Frontiers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-417402020-05-04T18:40:59Z https://eprints.nottingham.ac.uk/41740/ Subset-specific expression of toll-like receptors by bovine afferent lymph dendritic cells Werling, Dirk Hope, Jayne C. Siddiqui, Nazneen Widdison, Stephanie Russell, Chris Sopp, Paul Coffey, Tracey J. Within the ruminant system, several possibilities exist to generate dendritic cells migrating out from the tissue into the regional draining lymph nodes as afferent lymph dendritic cells (ALDCs). Here, we analyzed toll-like receptor (TLR) 1–10 mRNA expression by using quantitative real-time PCR in highly purified subsets of bovine ALDC. As TLR expression may be influenced by pathogens or vaccines and their adjuvant, it is necessary to understand what TLRs are expressed in a steady-state system to elucidate specific differences and to potentially optimize targeted vaccines. In this study, we have assessed the TLR expression profiles of the four main bovine ALDC subsets [cDC1 and cDC2 (subsets 2–4)]. We demonstrate differences in TLR expression between the four subsets that may reflect the ability of these cells to respond to different pathogens or to respond to adjuvants. Frontiers 2017-04-03 Article PeerReviewed Werling, Dirk, Hope, Jayne C., Siddiqui, Nazneen, Widdison, Stephanie, Russell, Chris, Sopp, Paul and Coffey, Tracey J. (2017) Subset-specific expression of toll-like receptors by bovine afferent lymph dendritic cells. Frontiers in Veterinary Science, 4 . 44/1-44/6. ISSN 2297-1769 Toll-like receptors Dendritic cell Afferent lymph Dendritic cell subsets Bovine http://journal.frontiersin.org/article/10.3389/fvets.2017.00044/full doi:10.3389/fvets.2017.00044 doi:10.3389/fvets.2017.00044 |
| spellingShingle | Toll-like receptors Dendritic cell Afferent lymph Dendritic cell subsets Bovine Werling, Dirk Hope, Jayne C. Siddiqui, Nazneen Widdison, Stephanie Russell, Chris Sopp, Paul Coffey, Tracey J. Subset-specific expression of toll-like receptors by bovine afferent lymph dendritic cells |
| title | Subset-specific expression of toll-like receptors by bovine afferent lymph dendritic cells |
| title_full | Subset-specific expression of toll-like receptors by bovine afferent lymph dendritic cells |
| title_fullStr | Subset-specific expression of toll-like receptors by bovine afferent lymph dendritic cells |
| title_full_unstemmed | Subset-specific expression of toll-like receptors by bovine afferent lymph dendritic cells |
| title_short | Subset-specific expression of toll-like receptors by bovine afferent lymph dendritic cells |
| title_sort | subset-specific expression of toll-like receptors by bovine afferent lymph dendritic cells |
| topic | Toll-like receptors Dendritic cell Afferent lymph Dendritic cell subsets Bovine |
| url | https://eprints.nottingham.ac.uk/41740/ https://eprints.nottingham.ac.uk/41740/ https://eprints.nottingham.ac.uk/41740/ |