Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins
Although like proteins, polysaccharides are synthesized by enzymes, unlike proteins there is no template. This means that they are polydisperse, do not generally have compact folded structures, and are often very large with greater nonideality behavior in solution. This chapter considers the relevan...
| Main Authors: | , , , , , , |
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| Format: | Book Section |
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Academic Press
2015
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| Online Access: | https://eprints.nottingham.ac.uk/41733/ |
| _version_ | 1848796340758249472 |
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| author | Harding, Stephen E. Adams, Gary G. Almutairi, Fahad Alzahrani, Qushmua Erten, Tayyibe Samil Kök, M. Gillis, Richard B. |
| author_facet | Harding, Stephen E. Adams, Gary G. Almutairi, Fahad Alzahrani, Qushmua Erten, Tayyibe Samil Kök, M. Gillis, Richard B. |
| author_sort | Harding, Stephen E. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Although like proteins, polysaccharides are synthesized by enzymes, unlike proteins there is no template. This means that they are polydisperse, do not generally have compact folded structures, and are often very large with greater nonideality behavior in solution. This chapter considers the relevant analytical ultracentrifuge methodology available for characterizing these and related carbohydrate-based systems and information this methodology supplies, in terms of sizes, shapes, and interactions using a comprehensive range of examples, including glycoconjugates and lignins. The relevance and potential of recent software developments such as SEDFIT-MSTAR, the Extended Fujita algorithm, and HYDFIT are considered. |
| first_indexed | 2025-11-14T19:46:26Z |
| format | Book Section |
| id | nottingham-41733 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:46:26Z |
| publishDate | 2015 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-417332020-05-04T17:16:27Z https://eprints.nottingham.ac.uk/41733/ Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins Harding, Stephen E. Adams, Gary G. Almutairi, Fahad Alzahrani, Qushmua Erten, Tayyibe Samil Kök, M. Gillis, Richard B. Although like proteins, polysaccharides are synthesized by enzymes, unlike proteins there is no template. This means that they are polydisperse, do not generally have compact folded structures, and are often very large with greater nonideality behavior in solution. This chapter considers the relevant analytical ultracentrifuge methodology available for characterizing these and related carbohydrate-based systems and information this methodology supplies, in terms of sizes, shapes, and interactions using a comprehensive range of examples, including glycoconjugates and lignins. The relevance and potential of recent software developments such as SEDFIT-MSTAR, the Extended Fujita algorithm, and HYDFIT are considered. Academic Press 2015-09-24 Book Section PeerReviewed Harding, Stephen E., Adams, Gary G., Almutairi, Fahad, Alzahrani, Qushmua, Erten, Tayyibe, Samil Kök, M. and Gillis, Richard B. (2015) Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins. In: Analytical Ultracentrifugation. Methods in Enzymology (562). Academic Press, pp. 391-439. ISBN 9780128029084 Size; Shape; Interactions; SEDFIT-MSTAR; Extended Fujita; Flexibility http://www.sciencedirect.com/science/article/pii/S0076687915004036 doi:10.1016/bs.mie.2015.06.043 doi:10.1016/bs.mie.2015.06.043 |
| spellingShingle | Size; Shape; Interactions; SEDFIT-MSTAR; Extended Fujita; Flexibility Harding, Stephen E. Adams, Gary G. Almutairi, Fahad Alzahrani, Qushmua Erten, Tayyibe Samil Kök, M. Gillis, Richard B. Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins |
| title | Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins |
| title_full | Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins |
| title_fullStr | Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins |
| title_full_unstemmed | Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins |
| title_short | Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins |
| title_sort | ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins |
| topic | Size; Shape; Interactions; SEDFIT-MSTAR; Extended Fujita; Flexibility |
| url | https://eprints.nottingham.ac.uk/41733/ https://eprints.nottingham.ac.uk/41733/ https://eprints.nottingham.ac.uk/41733/ |