Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge
Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macrom...
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| Format: | Article |
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MDPI
2015
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| Online Access: | https://eprints.nottingham.ac.uk/41717/ |
| _version_ | 1848796338895978496 |
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| author | Harding, Stephen Gillis, Richard Almutairi, Fahad Erten, Tayyibe Kök, M. Adams, Gary |
| author_facet | Harding, Stephen Gillis, Richard Almutairi, Fahad Erten, Tayyibe Kök, M. Adams, Gary |
| author_sort | Harding, Stephen |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10−12 M to 10−1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interactions. |
| first_indexed | 2025-11-14T19:46:24Z |
| format | Article |
| id | nottingham-41717 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:46:24Z |
| publishDate | 2015 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-417172020-05-04T17:04:59Z https://eprints.nottingham.ac.uk/41717/ Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge Harding, Stephen Gillis, Richard Almutairi, Fahad Erten, Tayyibe Kök, M. Adams, Gary Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10−12 M to 10−1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interactions. MDPI 2015-03-06 Article PeerReviewed Harding, Stephen, Gillis, Richard, Almutairi, Fahad, Erten, Tayyibe, Kök, M. and Adams, Gary (2015) Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge. Biology, 4 (1). pp. 237-250. ISSN 2079-7737 protein; carbohydrate; nucleic acid; interaction; hydrodynamics http://www.mdpi.com/2079-7737/4/1/237 doi:10.3390/biology4010237 doi:10.3390/biology4010237 |
| spellingShingle | protein; carbohydrate; nucleic acid; interaction; hydrodynamics Harding, Stephen Gillis, Richard Almutairi, Fahad Erten, Tayyibe Kök, M. Adams, Gary Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge |
| title | Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge |
| title_full | Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge |
| title_fullStr | Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge |
| title_full_unstemmed | Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge |
| title_short | Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge |
| title_sort | recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge |
| topic | protein; carbohydrate; nucleic acid; interaction; hydrodynamics |
| url | https://eprints.nottingham.ac.uk/41717/ https://eprints.nottingham.ac.uk/41717/ https://eprints.nottingham.ac.uk/41717/ |