A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic

Under investigation are the structural properties of gum arabic, an industrially important biopolymer for use as a stabilizer or in drug delivery, using Analytical Ultracentrifugation—a well-established, matrix-free probe for macromolecular size and shape. These results are combined with chromatogra...

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Main Authors: Gillis, Richard B., Adams, Gary G., Alzahrani, Qushmua, Harding, Stephen E., Allen Bush, C.
Format: Article
Published: Wiley 2016
Subjects:
Online Access:https://eprints.nottingham.ac.uk/41700/
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author Gillis, Richard B.
Adams, Gary G.
Alzahrani, Qushmua
Harding, Stephen E.
Allen Bush, C.
author_facet Gillis, Richard B.
Adams, Gary G.
Alzahrani, Qushmua
Harding, Stephen E.
Allen Bush, C.
author_sort Gillis, Richard B.
building Nottingham Research Data Repository
collection Online Access
description Under investigation are the structural properties of gum arabic, an industrially important biopolymer for use as a stabilizer or in drug delivery, using Analytical Ultracentrifugation—a well-established, matrix-free probe for macromolecular size and shape. These results are combined with chromatographically-coupled methods (multi-angle light scattering, differential press imbalance viscometry) to provide a global analysis of its structure in varying ionic strength conditions. This analysis indicates that gum Arabic may have a compact, elliptical structure in solution, the significance of which for biotechnological use is indicated. This modelling method can be applied to other biopolymers and synthetic polymers.
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spelling nottingham-417002020-05-04T20:01:07Z https://eprints.nottingham.ac.uk/41700/ A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic Gillis, Richard B. Adams, Gary G. Alzahrani, Qushmua Harding, Stephen E. Allen Bush, C. Under investigation are the structural properties of gum arabic, an industrially important biopolymer for use as a stabilizer or in drug delivery, using Analytical Ultracentrifugation—a well-established, matrix-free probe for macromolecular size and shape. These results are combined with chromatographically-coupled methods (multi-angle light scattering, differential press imbalance viscometry) to provide a global analysis of its structure in varying ionic strength conditions. This analysis indicates that gum Arabic may have a compact, elliptical structure in solution, the significance of which for biotechnological use is indicated. This modelling method can be applied to other biopolymers and synthetic polymers. Wiley 2016-09 Article PeerReviewed Gillis, Richard B., Adams, Gary G., Alzahrani, Qushmua, Harding, Stephen E. and Allen Bush, C. (2016) A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic. Biopolymers, 105 (9). pp. 618-625. ISSN 1097-0282 acacia; AUC; characterization; conformation; gum arabic; hydrodynamic; sedimentation velocity; ultracentrifugation http://onlinelibrary.wiley.com/doi/10.1002/bip.22823/abstract doi:10.1002/bip.22823 doi:10.1002/bip.22823
spellingShingle acacia; AUC; characterization; conformation; gum arabic; hydrodynamic; sedimentation velocity; ultracentrifugation
Gillis, Richard B.
Adams, Gary G.
Alzahrani, Qushmua
Harding, Stephen E.
Allen Bush, C.
A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic
title A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic
title_full A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic
title_fullStr A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic
title_full_unstemmed A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic
title_short A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic
title_sort novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic
topic acacia; AUC; characterization; conformation; gum arabic; hydrodynamic; sedimentation velocity; ultracentrifugation
url https://eprints.nottingham.ac.uk/41700/
https://eprints.nottingham.ac.uk/41700/
https://eprints.nottingham.ac.uk/41700/