The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health

Scope Iron is an essential nutrient. However, in animal models, excess unabsorbed dietary iron residing within the colonic lumen has been shown to exacerbate inflammatory bowel disease and intestinal cancer. Therefore, the aims of this study were to screen a panel of alginates to identify a thera...

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Main Authors: Horniblow, Richard D., Latunde-Dada, Gladys O., Harding, Stephen E., Schneider, Melanie, Almutairi, Fahad M., Sahni, Manroy, Bhatti, Ahsan, Ludwig, Christian, Norton, Ian T., Iqbal, Tariq H., Tselepis, Chris
Format: Article
Published: Wiley 2016
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Online Access:https://eprints.nottingham.ac.uk/41693/
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author Horniblow, Richard D.
Latunde-Dada, Gladys O.
Harding, Stephen E.
Schneider, Melanie
Almutairi, Fahad M.
Sahni, Manroy
Bhatti, Ahsan
Ludwig, Christian
Norton, Ian T.
Iqbal, Tariq H.
Tselepis, Chris
author_facet Horniblow, Richard D.
Latunde-Dada, Gladys O.
Harding, Stephen E.
Schneider, Melanie
Almutairi, Fahad M.
Sahni, Manroy
Bhatti, Ahsan
Ludwig, Christian
Norton, Ian T.
Iqbal, Tariq H.
Tselepis, Chris
author_sort Horniblow, Richard D.
building Nottingham Research Data Repository
collection Online Access
description Scope Iron is an essential nutrient. However, in animal models, excess unabsorbed dietary iron residing within the colonic lumen has been shown to exacerbate inflammatory bowel disease and intestinal cancer. Therefore, the aims of this study were to screen a panel of alginates to identify a therapeutic that can chelate this pool of iron and thus be beneficial for intestinal health. Methods and results Using several in vitro intestinal models, it is evident that only one alginate (Manucol LD) of the panel tested was able to inhibit intracellular iron accumulation as assessed by iron-mediated ferritin induction, transferrin receptor expression, intracellular 59Fe concentrations, and iron flux across a Caco-2 monolayer. Additionally, Manucol LD suppressed iron absorption in mice, which was associated with increased fecal iron levels indicating iron chelation within the gastrointestinal tract. Furthermore, the bioactivity of Manucol LD was found to be highly dependent on both its molecular weight and its unique compositional sequence. Conclusion Manucol LD could be useful for the chelation of this detrimental pool of unabsorbed iron and it could be fortified in foods to enhance intestinal health.
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spelling nottingham-416932020-05-04T20:01:12Z https://eprints.nottingham.ac.uk/41693/ The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health Horniblow, Richard D. Latunde-Dada, Gladys O. Harding, Stephen E. Schneider, Melanie Almutairi, Fahad M. Sahni, Manroy Bhatti, Ahsan Ludwig, Christian Norton, Ian T. Iqbal, Tariq H. Tselepis, Chris Scope Iron is an essential nutrient. However, in animal models, excess unabsorbed dietary iron residing within the colonic lumen has been shown to exacerbate inflammatory bowel disease and intestinal cancer. Therefore, the aims of this study were to screen a panel of alginates to identify a therapeutic that can chelate this pool of iron and thus be beneficial for intestinal health. Methods and results Using several in vitro intestinal models, it is evident that only one alginate (Manucol LD) of the panel tested was able to inhibit intracellular iron accumulation as assessed by iron-mediated ferritin induction, transferrin receptor expression, intracellular 59Fe concentrations, and iron flux across a Caco-2 monolayer. Additionally, Manucol LD suppressed iron absorption in mice, which was associated with increased fecal iron levels indicating iron chelation within the gastrointestinal tract. Furthermore, the bioactivity of Manucol LD was found to be highly dependent on both its molecular weight and its unique compositional sequence. Conclusion Manucol LD could be useful for the chelation of this detrimental pool of unabsorbed iron and it could be fortified in foods to enhance intestinal health. Wiley 2016-09 Article PeerReviewed Horniblow, Richard D., Latunde-Dada, Gladys O., Harding, Stephen E., Schneider, Melanie, Almutairi, Fahad M., Sahni, Manroy, Bhatti, Ahsan, Ludwig, Christian, Norton, Ian T., Iqbal, Tariq H. and Tselepis, Chris (2016) The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health. Molecular Nutrition & Food Research, 60 (9). pp. 2098-2108. ISSN 1613-4133 Absorption; Alginate; Chelation; Intestinal; Iron http://onlinelibrary.wiley.com/doi/10.1002/mnfr.201500882/abstract doi:10.1002/mnfr.201500882 doi:10.1002/mnfr.201500882
spellingShingle Absorption; Alginate; Chelation; Intestinal; Iron
Horniblow, Richard D.
Latunde-Dada, Gladys O.
Harding, Stephen E.
Schneider, Melanie
Almutairi, Fahad M.
Sahni, Manroy
Bhatti, Ahsan
Ludwig, Christian
Norton, Ian T.
Iqbal, Tariq H.
Tselepis, Chris
The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health
title The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health
title_full The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health
title_fullStr The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health
title_full_unstemmed The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health
title_short The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health
title_sort chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health
topic Absorption; Alginate; Chelation; Intestinal; Iron
url https://eprints.nottingham.ac.uk/41693/
https://eprints.nottingham.ac.uk/41693/
https://eprints.nottingham.ac.uk/41693/