Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions

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Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions

This article reviews current methods for the reliable heterologous overexpression in Escherichia coli and purification of milligram quantities of bacterial membrane sensor kinase (MSK) proteins belonging to the two-component signal transduction family of integral membrane proteins. Many of these met...

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Bibliographic Details
Main Authors:	Hussain, Rohanah, Harding, Stephen E., Hughes, Charlotte S., Ma, Pikyee, Patching, Simon G., Edara, Shalini, Siligardi, Giuliano, Henderson, Peter J.F., Phillips-Jones, Mary K.
Format:	Article
Published:	Portland Press 2016
Online Access:	https://eprints.nottingham.ac.uk/41690/

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