Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans
Polysaccharides, the most abundant biopolymers, are required for a host of activities in lower organisms, animals, and plants. Their solution characterization is challenging due to their complex shape, heterogeneity, and size. Here, recently developed data analysis approaches were applied for tradit...
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Springer
2017
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| Online Access: | https://eprints.nottingham.ac.uk/41686/ |
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| author | Gillis, Richard B. Adams, Gary G. Besong, David T.M. Machová, Eva Ebringerová, Anna Rowe, Arthur J. Harding, Stephen E. Patel, Trushar R. |
| author_facet | Gillis, Richard B. Adams, Gary G. Besong, David T.M. Machová, Eva Ebringerová, Anna Rowe, Arthur J. Harding, Stephen E. Patel, Trushar R. |
| author_sort | Gillis, Richard B. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Polysaccharides, the most abundant biopolymers, are required for a host of activities in lower organisms, animals, and plants. Their solution characterization is challenging due to their complex shape, heterogeneity, and size. Here, recently developed data analysis approaches were applied for traditional sedimentation equilibrium and velocity methods in order to investigate the molar mass distribution(s) of a subtype of polysaccharide, namely, mannans from four Candida spp. The molecular weight distributions of these mannans were studied using two recently developed equilibrium approaches: SEDFIT-MSTAR and MULTISIG, resulting in corroboratory distribution profiles. Additionally, sedimentation velocity data for all four mannans, analyzed using ls-g*(s) and Extended Fujita approaches, suggest that two of the fungal mannans (FM-1 and FM-3) have a unimodal distribution of molecular species whereas two others (FM-2 and FM-4) displayed bi-modal and broad distributions, respectively: this demonstrates considerable molecular heterogeneity in these polysaccharides, consistent with previous observations of mannans and polysaccharides in general. These methods not only have applications for the characterization of mannans but for other biopolymers such as polysaccharides, DNA, and proteins (including intrinsically disordered proteins). |
| first_indexed | 2025-11-14T19:46:16Z |
| format | Article |
| id | nottingham-41686 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:46:16Z |
| publishDate | 2017 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-416862020-05-04T19:57:57Z https://eprints.nottingham.ac.uk/41686/ Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans Gillis, Richard B. Adams, Gary G. Besong, David T.M. Machová, Eva Ebringerová, Anna Rowe, Arthur J. Harding, Stephen E. Patel, Trushar R. Polysaccharides, the most abundant biopolymers, are required for a host of activities in lower organisms, animals, and plants. Their solution characterization is challenging due to their complex shape, heterogeneity, and size. Here, recently developed data analysis approaches were applied for traditional sedimentation equilibrium and velocity methods in order to investigate the molar mass distribution(s) of a subtype of polysaccharide, namely, mannans from four Candida spp. The molecular weight distributions of these mannans were studied using two recently developed equilibrium approaches: SEDFIT-MSTAR and MULTISIG, resulting in corroboratory distribution profiles. Additionally, sedimentation velocity data for all four mannans, analyzed using ls-g*(s) and Extended Fujita approaches, suggest that two of the fungal mannans (FM-1 and FM-3) have a unimodal distribution of molecular species whereas two others (FM-2 and FM-4) displayed bi-modal and broad distributions, respectively: this demonstrates considerable molecular heterogeneity in these polysaccharides, consistent with previous observations of mannans and polysaccharides in general. These methods not only have applications for the characterization of mannans but for other biopolymers such as polysaccharides, DNA, and proteins (including intrinsically disordered proteins). Springer 2017-04 Article PeerReviewed Gillis, Richard B., Adams, Gary G., Besong, David T.M., Machová, Eva, Ebringerová, Anna, Rowe, Arthur J., Harding, Stephen E. and Patel, Trushar R. (2017) Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans. European Biophysics Journal, 46 (3). pp. 235-245. ISSN 0175-7571 AUC; Extended Fujita approach; MULTISIG; SEDFIT-MSTAR; Sedimentation http://link.springer.com/article/10.1007%2Fs00249-016-1159-5 doi:10.1007/s00249-016-1159-5 doi:10.1007/s00249-016-1159-5 |
| spellingShingle | AUC; Extended Fujita approach; MULTISIG; SEDFIT-MSTAR; Sedimentation Gillis, Richard B. Adams, Gary G. Besong, David T.M. Machová, Eva Ebringerová, Anna Rowe, Arthur J. Harding, Stephen E. Patel, Trushar R. Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans |
| title | Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans |
| title_full | Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans |
| title_fullStr | Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans |
| title_full_unstemmed | Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans |
| title_short | Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans |
| title_sort | application of novel analytical ultracentrifuge analysis to solutions of fungal mannans |
| topic | AUC; Extended Fujita approach; MULTISIG; SEDFIT-MSTAR; Sedimentation |
| url | https://eprints.nottingham.ac.uk/41686/ https://eprints.nottingham.ac.uk/41686/ https://eprints.nottingham.ac.uk/41686/ |