IL-36α induces maturation of Th1-inducing human MDDC and synergises with IFN-γ to induce high surface expression of CD14 and CD11c
We show that IL-36α induced maturation of human MDDCs and stimulated differentiation of IFN-γ producing (Type 1) CD3+ lymphocytes but was not as effective as IL-36β in doing so. For the first time, we also show that IL-36α induced expression of CD14 by MDDCs and this was highly potentiated by co-cul...
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| Format: | Article |
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Elsevier
2015
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| Online Access: | https://eprints.nottingham.ac.uk/41588/ |
| _version_ | 1848796309615542272 |
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| author | Higgins, John Mutamba, Shilla Mahida, Yashwant R. Barrow, Paul Foster, Neil |
| author_facet | Higgins, John Mutamba, Shilla Mahida, Yashwant R. Barrow, Paul Foster, Neil |
| author_sort | Higgins, John |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | We show that IL-36α induced maturation of human MDDCs and stimulated differentiation of IFN-γ producing (Type 1) CD3+ lymphocytes but was not as effective as IL-36β in doing so. For the first time, we also show that IL-36α induced expression of CD14 by MDDCs and this was highly potentiated by co-cultured with IFN-γ. In contrast, lipopolysaccharide (LPS) did not increase CD14 expression by MDDCs, suggesting that if MDDCs represent a physiologically relevant population in vivo, they need to be stimulated by relevant inflammatory cytokines prior to CD14 expression and detection of LPS, expressed by Gram negative bacteria. IFN-γ synergised with IL-36α to restore the high levels of CD11c expression by MDDCs, which was reduced by culture with these cytokines in isolation. IL-36α/IFN-γ synergy also correlated with increased binding of the opsonic complement protein (iC3b) to MDDCs. However although IL-36α increased the phagocytic capacity of MDDCs for Salmonella Typhimurium 4/74 this was not synergistically increased by IFN-γ (P > 0.05).
In conclusion we report the hitherto unknown effects of IL-36α on the innate cell function of human MDDCs. |
| first_indexed | 2025-11-14T19:45:56Z |
| format | Article |
| id | nottingham-41588 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:45:56Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-415882020-05-04T17:05:42Z https://eprints.nottingham.ac.uk/41588/ IL-36α induces maturation of Th1-inducing human MDDC and synergises with IFN-γ to induce high surface expression of CD14 and CD11c Higgins, John Mutamba, Shilla Mahida, Yashwant R. Barrow, Paul Foster, Neil We show that IL-36α induced maturation of human MDDCs and stimulated differentiation of IFN-γ producing (Type 1) CD3+ lymphocytes but was not as effective as IL-36β in doing so. For the first time, we also show that IL-36α induced expression of CD14 by MDDCs and this was highly potentiated by co-cultured with IFN-γ. In contrast, lipopolysaccharide (LPS) did not increase CD14 expression by MDDCs, suggesting that if MDDCs represent a physiologically relevant population in vivo, they need to be stimulated by relevant inflammatory cytokines prior to CD14 expression and detection of LPS, expressed by Gram negative bacteria. IFN-γ synergised with IL-36α to restore the high levels of CD11c expression by MDDCs, which was reduced by culture with these cytokines in isolation. IL-36α/IFN-γ synergy also correlated with increased binding of the opsonic complement protein (iC3b) to MDDCs. However although IL-36α increased the phagocytic capacity of MDDCs for Salmonella Typhimurium 4/74 this was not synergistically increased by IFN-γ (P > 0.05). In conclusion we report the hitherto unknown effects of IL-36α on the innate cell function of human MDDCs. Elsevier 2015-04-30 Article PeerReviewed Higgins, John, Mutamba, Shilla, Mahida, Yashwant R., Barrow, Paul and Foster, Neil (2015) IL-36α induces maturation of Th1-inducing human MDDC and synergises with IFN-γ to induce high surface expression of CD14 and CD11c. Human Immunology, 76 (4). pp. 245-253. ISSN 1879-1166 Dendritic cell; IL-36R; IL-36α; IFN-γ; CD209 https://doi.org/10.1016/j.humimm.2015.01.012 doi:10.1016/j.humimm.2015.01.012 doi:10.1016/j.humimm.2015.01.012 |
| spellingShingle | Dendritic cell; IL-36R; IL-36α; IFN-γ; CD209 Higgins, John Mutamba, Shilla Mahida, Yashwant R. Barrow, Paul Foster, Neil IL-36α induces maturation of Th1-inducing human MDDC and synergises with IFN-γ to induce high surface expression of CD14 and CD11c |
| title | IL-36α induces maturation of Th1-inducing human MDDC and synergises with IFN-γ to induce high surface expression of CD14 and CD11c |
| title_full | IL-36α induces maturation of Th1-inducing human MDDC and synergises with IFN-γ to induce high surface expression of CD14 and CD11c |
| title_fullStr | IL-36α induces maturation of Th1-inducing human MDDC and synergises with IFN-γ to induce high surface expression of CD14 and CD11c |
| title_full_unstemmed | IL-36α induces maturation of Th1-inducing human MDDC and synergises with IFN-γ to induce high surface expression of CD14 and CD11c |
| title_short | IL-36α induces maturation of Th1-inducing human MDDC and synergises with IFN-γ to induce high surface expression of CD14 and CD11c |
| title_sort | il-36α induces maturation of th1-inducing human mddc and synergises with ifn-γ to induce high surface expression of cd14 and cd11c |
| topic | Dendritic cell; IL-36R; IL-36α; IFN-γ; CD209 |
| url | https://eprints.nottingham.ac.uk/41588/ https://eprints.nottingham.ac.uk/41588/ https://eprints.nottingham.ac.uk/41588/ |